The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cysteine residues 110 and 187 are essential for the formation of correct structure in bovine rhodopsin.
Open accessAbstract
To investigate the role of different cysteine residues in bovine rhodopsin, a series of mutants were prepared in which the cysteine residues were systematically replaced by serines. The mutant genes were expressed in monkey kidney cells (COS-1) and the mutant opsins were evaluated for their levels of expression, glycosylation patterns, and ability to form the chromophore characteristic of rhodopsin and to activate transducin. Substitution of the three cytoplasmic cysteines (Cys-316, Cys-322, and Cys-323) and the four membrane-embedded cysteines (Cys-140, Cys-167, Cys-222, and Cys-264) produced proteins with wild-type phenotype. Also, single substitutions of Cys-185 gave rise to a wild-type phenotype. In contrast, substitution of the three intradiscal cysteines (Cys-110, Cys-185, and Cys-187) or single substitution of Cys-110 or Cys-187 gave proteins that were expressed at reduced levels, glycosylated abnormally, and unable to bind 11-cis-retinal. Thus, of the 10 cysteines in bovine rhodopsin, only intradiscal Cys-110 and Cys-187 are essential for the correct tertiary structure of the protein.
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Cites
The Molecular Basis of Visual Excitation
WALD, G. (1968) · Nature · doi:10.1038/219800a0 · Cited by 824
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The cloning of a family of genes that encode the melanocortin receptors
Mountjoy, K. G., Robbins, L. S., Mortrud, M. T., & Cone, R. D. (1992) · Science · doi:10.1126/science.1325670 · Cited by 1,635
Melanopsin: An opsin in melanophores, brain, and eye
Provencio, I., Jiang, G., De Grip, W. J., Hayes, W. P., & Rollag, M. D. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.1.340 · Cited by 975
McBee, J. K., Palczewski, K., Baehr, W., & Pepperberg, D. R. (2001) · Progress in Retinal and Eye Research · doi:10.1016/s1350-9462(01)00002-7 · Cited by 366
Nathans, J. (1990) · Biochemistry · doi:10.1021/bi00493a034 · Cited by 323
Metadata
- Authors
- Karnik, S. S., Sakmar, T. P., Chen, H. B., & Khorana, H. G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1988-11
- Volume
- 85
- Issue
- 22
- Pages
- 8459-8463
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1993620919
- Cited by
- 414
- References
- 1
- FWCI
- 5.64
- Type
- journal article
- Language
- en
- Open access
- green
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