The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.
Open accessAbstract
The highly thermostable DNA polymerase from Thermus aquaticus (Taq) is ideal for both manual and automated DNA sequencing because it is fast, highly processive, has little or no 3'-exonuclease activity, and is active over a broad range of temperatures. Sequencing protocols are presented that produce readable extension products greater than 1000 bases having uniform band intensities. A combination of high reaction temperatures and the base analog 7-deaza-2'-deoxyguanosine was used to sequence through G + C-rich DNA and to resolve gel compressions. We modified the polymerase chain reaction (PCR) conditions for direct DNA sequencing of asymmetric PCR products without intermediate purification by using Taq DNA polymerase. The coupling of template preparation by asymmetric PCR and direct sequencing should facilitate automation for large-scale sequencing projects.
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Citations
Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Self-citationSaiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Cited by
Molecular cloning and expression of brain-derived neurotrophic factor
Leibrock, J., Lottspeich, F., Hohn, A., Hofer, M., Hengerer, B., Masiakowski, P., Thoenen, H., & Barde, Y.-A. (1989) · Nature · doi:10.1038/341149a0 · Cited by 1,457
Hohn, A., Leibrock, J., Bailey, K., & Barde, Y.-A. (1990) · Nature · doi:10.1038/344339a0 · Cited by 1,145
Tannin, G. M., Agarwal, A. K., Monder, C., New, M. I., & White, P. C. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55351-5 · Cited by 436
Effects of Amplification Facilitators on Diagnostic PCR in the Presence of Blood, Feces, and Meat
Abu Al-Soud, W., & Rådström, P. (2000) · Journal of Clinical Microbiology · doi:10.1128/jcm.38.12.4463-4470.2000 · Cited by 398
The Use and Implications of Ribosomal DNA Sequencing for the Discrimination of Digenean Species
Nolan, M. J., & Cribb, T. H. (2005) · Advances in Parasitology · doi:10.1016/s0065-308x(05)60002-4 · Cited by 273
Metadata
- Authors
- Innis, M. A., Myambo, K. B., Gelfand, D. H., & Brow, M. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1988-12
- Volume
- 85
- Issue
- 24
- Pages
- 9436-9440
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2139767135
- Cited by
- 775
- References
- 2
- FWCI
- 19.69
- Type
- journal article
- Language
- en
- Open access
- green
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- Innis, M. A., Myambo, K. B., Gelfand, D. H., & Brow, M. A. (1988). DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.85.24.9436
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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