Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Abstract
A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus , greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified. In addition, the method was used to amplify and detect a target DNA molecule present only once in a sample of 10 5 cells.
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- Authors
- Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A.
- Affiliations
- Cetus Corporation, Department of Human Genetics, Emeryville, CA 94608.
- Cetus Corporation, Department of Microbial Genetics, Emeryville, CA 94608.
- Venue
- Science
- Published
- 1988-01-29
- Volume
- 239
- Issue
- 4839
- Pages
- 487-491
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2032118018
- Cited by
- 17,719
- References
- 19
- Citations by year
- 2026: 85 · 2025: 101 · 2024: 109 · 2023: 181 · 2022: 113 · 2021: 150 · 2020: 161 · 2019: 152 · 2018: 161 · 2017: 165 · 2016: 210 · 2015: 199 · 2014: 258 · 2013: 238 · 2012: 236
- FWCI
- 504.53
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Keywords
- PCR, thermostable DNA polymerase, Thermus aquaticus, DNA amplification
- MeSH terms
- Cloning, Molecular, DNA, DNA-Directed DNA Polymerase, DNA, Recombinant, Electrophoresis, Agar Gel, Globins, Hot Temperature, Humans, Nucleic Acid Denaturation, Nucleic Acid Hybridization, RNA, Thermus, Nucleic Acid Amplification Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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