Cited by 17,719Molecular Biology Techniques and ApplicationsDNA and Nucleic Acid ChemistryGenomics and Phylogenetic Studies

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

Saiki, Randall K., Gelfand, David H., Stoffel, Susanne, Scharf, Stephen J., Higuchi, Russell, Horn, Glenn T., Mullis, Kary B., Erlich, Henry A.·1988·Science

Abstract

A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus , greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified. In addition, the method was used to amplify and detect a target DNA molecule present only once in a sample of 10 5 cells.

Integrity signals

Registry

Crossref · 10.1126/science.2448875

Cites

19 of 19 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Cites

Flagged first, then by citations

Research works printed but not linked: 1

  1. Tuan, D., Proceedings of the National Academy of Sciences of the United States of America 80: 6937 (1983).

Cited by

Sorted by citations

Authors
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A.
Affiliations
  • Cetus Corporation, Department of Human Genetics, Emeryville, CA 94608.
  • Cetus Corporation, Department of Microbial Genetics, Emeryville, CA 94608.
Venue
Science
Published
1988-01-29
Volume
239
Issue
4839
Pages
487-491
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2032118018
Cited by
17,719
References
19
Citations by year
2026: 85 · 2025: 101 · 2024: 109 · 2023: 181 · 2022: 113 · 2021: 150 · 2020: 161 · 2019: 152 · 2018: 161 · 2017: 165 · 2016: 210 · 2015: 199 · 2014: 258 · 2013: 238 · 2012: 236
FWCI
504.53
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords
PCR, thermostable DNA polymerase, Thermus aquaticus, DNA amplification
MeSH terms
Cloning, Molecular, DNA, DNA-Directed DNA Polymerase, DNA, Recombinant, Electrophoresis, Agar Gel, Globins, Hot Temperature, Humans, Nucleic Acid Denaturation, Nucleic Acid Hybridization, RNA, Thermus, Nucleic Acid Amplification Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review