Virion basic phosphoprotein from human cytomegalovirus contains O-linked N-acetylglucosamine.
Open accessAbstract
A 149-kDa virion protein of human strains of cytomegalovirus is the principal acceptor for galactose added in vitro by bovine milk galactosyltransferase. Peptide comparisons with other biochemical characteristics of the galactosylated protein identified it as the virus-encoded basic phosphoprotein. This protein is an abundant constituent of the virion and is located in the tegument region, between the capsid and the envelope, rather than in the envelope layer with the recognized virion glycoproteins. The galactosylated carbohydrate was resistant to a commercial preparation of endoglycosidase F but was sensitive to removal by alkali-induced beta-elimination, indicating an O-linkage to the protein. Chromatographic and electrophoretic determinations identified the beta-eliminated material as the alditol of Gal beta 1-4GlcNAc, establishing that the human cytomegalovirus virion basic phosphoprotein contains single O-linked residues of N-acetylglucosamine.
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- Authors
- Benko, D. M., Haltiwanger, R. S., Hart, G. W., & Gibson, W.
- Affiliations
- Johns Hopkins University (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1988-04
- Volume
- 85
- Issue
- 8
- Pages
- 2573-2577
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC280039
- OpenAlex
- W2057977009
- Cited by
- 66
- References
- 48
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 2 · 2021: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 1
- FWCI
- 2.24
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (CA-42486)
- National Institute of Allergy and Infectious Diseases (AI22711, AI 13718)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Keywords
- human cytomegalovirus, O-glycosylation, N-acetylglucosamine, galactosylation, galactosyltransferase, tegument proteins, β-elimination, viral glycoproteins
- MeSH terms
- Acetylglucosamine, Cytomegalovirus, Glucosamine, Isoelectric Point, Molecular Weight, Phosphoproteins, Virion
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