Cited by 66Glycosylation and Glycoproteins ResearchCytomegalovirus and herpesvirus researchViral Infectious Diseases and Gene Expression in Insects

Virion basic phosphoprotein from human cytomegalovirus contains O-linked N-acetylglucosamine.

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Benko, D M, Haltiwanger, R S, Hart, G W, Gibson, W·1988·Proceedings of the National Academy of Sciences

Abstract

A 149-kDa virion protein of human strains of cytomegalovirus is the principal acceptor for galactose added in vitro by bovine milk galactosyltransferase. Peptide comparisons with other biochemical characteristics of the galactosylated protein identified it as the virus-encoded basic phosphoprotein. This protein is an abundant constituent of the virion and is located in the tegument region, between the capsid and the envelope, rather than in the envelope layer with the recognized virion glycoproteins. The galactosylated carbohydrate was resistant to a commercial preparation of endoglycosidase F but was sensitive to removal by alkali-induced beta-elimination, indicating an O-linkage to the protein. Chromatographic and electrophoretic determinations identified the beta-eliminated material as the alditol of Gal beta 1-4GlcNAc, establishing that the human cytomegalovirus virion basic phosphoprotein contains single O-linked residues of N-acetylglucosamine.

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Crossref · 10.1073/pnas.85.8.2573

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Authors
Benko, D. M., Haltiwanger, R. S., Hart, G. W., & Gibson, W.
Affiliations
Johns Hopkins University (US)
Venue
Proceedings of the National Academy of Sciences
Published
1988-04
Volume
85
Issue
8
Pages
2573-2577
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2057977009
Cited by
66
References
48
Citations by year
2025: 1 · 2024: 1 · 2022: 2 · 2021: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 1
FWCI
2.24
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Cancer Institute (CA-42486)
  • National Institute of Allergy and Infectious Diseases (AI22711, AI 13718)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
  • Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Keywords
human cytomegalovirus, O-glycosylation, N-acetylglucosamine, galactosylation, galactosyltransferase, tegument proteins, β-elimination, viral glycoproteins
MeSH terms
Acetylglucosamine, Cytomegalovirus, Glucosamine, Isoelectric Point, Molecular Weight, Phosphoproteins, Virion

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