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A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions.
Open accessAbstract
We describe the construction of six strains of Escherichia coli with different mutations at the same coding position in the lacZ gene, which specifies the active site glutamic acid residue at position 461 in beta'-galactosidase. Each strain is Lac- and reverts to Lac+ only by restoring the glutamic acid codon. The strains have been designed so that each reverts via one of the six base substitutions. The set of strains allows detection of each transition and transversion simply by monitoring the Lac- to Lac+ frequency, as demonstrated here with characterized mutagens and mutator alleles. These strains are useful for rapidly determining the mutagenic specificity of mutagens at a single site, for detecting low levels of stimulation of certain base substitutions, for monitoring specific base changes in response to various experimental conditions or strain backgrounds, and for isolating new mutator strains.
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Cited by
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Domingo, E., & Holland, J. J. (1997) · Annual Review of Microbiology · doi:10.1146/annurev.micro.51.1.151 · Cited by 1,514
Domingo, E., Sheldon, J., & Perales, C. (2012) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.05023-11 · Cited by 1,009
Lee, H., Popodi, E., Tang, H., & Foster, P. L. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1210309109 · Cited by 681
Mechanisms of Stationary Phase Mutation: A Decade of Adaptive Mutation
Foster, P. L. (1999) · Annual Review of Genetics · doi:10.1146/annurev.genet.33.1.57 · Cited by 182
Bryant, J., Chewapreecha, C., & Bentley, S. D. (2012) · Future Microbiology · doi:10.2217/fmb.12.108 · Cited by 98
Metadata
- Authors
- Cupples, C. G., & Miller, J. H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-07
- Volume
- 86
- Issue
- 14
- Pages
- 5345-5349
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1997125979
- Cited by
- 448
- References
- 0
- FWCI
- 3.35
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions. https://app.cochat.ai/papers/10.1073/pnas.86.14.5345
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- Cupples, C. G., & Miller, J. H. (1989). A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.14.5345
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