The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources.
Open accessAbstract
Current efforts to map the human genome are focused on individual chromosomes or smaller regions and frequently rely on the use of somatic cell hybrids. We report the application of the polymerase chain reaction to direct amplification of human DNA from hybrid cells containing regions of the human genome in rodent cell backgrounds using primers directed to the human Alu repeat element. We demonstrate Alu-directed amplification of a fragment of the human HPRT gene from both hybrid cell and cloned DNA and identify through sequence analysis the Alu repeats involved in this amplification. We also demonstrate the application of this technique to identify the chromosomal locations of large fragments of the human X chromosome cloned in a yeast artificial chromosome and the general applicability of the method to the preparation of DNA probes from cloned human sequences. The technique allows rapid gene mapping and provides a simple method for the isolation and analysis of specific chromosomal regions.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
SINEs and LINEs: Highly repeated short and long interspersed sequences in mammalian genomes
Singer, M. F. (1982) · Cell · doi:10.1016/0092-8674(82)90194-5 · Cited by 431
Cited by
Legouis, R., Hardelin, J.-P., Levilliers, J., Claverie, J.-M., Compain, S., Wunderle, V., Millasseau, P., Le Paslier, D., Cohen, D., & Caterina, D. (1991) · Cell · doi:10.1016/0092-8674(91)90193-3 · Cited by 678
Rawson, R. B., Zelenski, N. G., Nijhawan, D., Ye, J., Sakai, J., Hasan, M. T., Chang, T. Y., Brown, M. S., & Goldstein, J. L. (1997) · Molecular Cell · doi:10.1016/s1097-2765(00)80006-4 · Cited by 429
Pentao, L., Wise, C. A., Chinault, A. C., Patel, P. I., & Lupski, J. R. (1992) · Nature Genetics · doi:10.1038/ng1292-292 · Cited by 386
Welsh, J., Petersen, C., & McClelland, M. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.2.303 · Cited by 355
Prototypic sequences for human repetitive DNA
Jurka, J., Walichiewicz, J., & Milosavljevic, A. (1992) · Journal of Molecular Evolution · doi:10.1007/bf00161166 · Cited by 108
Metadata
- Authors
- Nelson, D. L., Ledbetter, S. A., Corbo, L., Victoria, M. F., Ramírez-Solis, R., Webster, T. D., Ledbetter, D. H., & Caskey, C. T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-09
- Volume
- 86
- Issue
- 17
- Pages
- 6686-6690
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2072180899
- Cited by
- 577
- References
- 1
- FWCI
- 34.56
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources. https://app.cochat.ai/papers/10.1073/pnas.86.17.6686
- The paper
- Nelson, D. L., Ledbetter, S. A., Corbo, L., Victoria, M. F., Ramírez-Solis, R., Webster, T. D., Ledbetter, D. H., & Caskey, C. T. (1989). Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.17.6686
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
