The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells.
Open accessAbstract
Two forms of erythropoietin, EPO-bi and EPO-tetra, with different biological activities were isolated from the culture medium of a recombinant Chinese hamster ovary cell line, B8-300, into which the human erythropoietin gene had been introduced. EPO-bi, an unusual form, showed only one-seventh the in vivo activity and 3 times higher in vitro activity of the previously described recombinant human EPO (standard EPO). In contrast, EPO-tetra showed both in vivo and in vitro activities comparable to those of the standard EPO. EPO-bi, EPO-tetra, and the standard EPO had the same amino acid composition and immunoreactivity. However, structural analyses of their N-linked sugar chains revealed that EPO-bi contains the biantennary complex type as the major sugar chain, while EPO-tetra and the standard EPO contain the tetraantennary complex type as the major sugar chain. From examination of various preparations of recombinant human EPO, we found a positive correlation between the in vivo activity of EPO and the ratio of tetraantennary to biantennary oligosaccharides. These results suggest that higher branching of the N-linked sugar chains is essential for effective expression of in vivo biological activity of EPO.
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Cites
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Self-citation
Cited by
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“The size, number, branching and degree of sialylation of N-glycans can generate numerous glycoforms of a single polypeptide such as erythropoietin (629) (630) (631) (632) (633) (634) (635) (636) (637) (638) (639) (640) or GM-CSF (641) (642) (643) (644) .”
BackgroundProtein glycosylation: Structural and functional aspects
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Comparison of Yeasts as Hosts for Recombinant Protein Production
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Metadata
- Authors
- Takeuchi, M., Inoue, N., Strickland, T. W., Kubota, M., Wada, M., Shimizu, R., Hoshi, S., Kozutsumi, H., Takasaki, S., & Kobata, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-10
- Volume
- 86
- Issue
- 20
- Pages
- 7819-7822
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2021482099
- Cited by
- 275
- References
- 22
- FWCI
- 2.98
- Type
- journal article
- Language
- en
- Open access
- green
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- Takeuchi, M., Inoue, N., Strickland, T. W., Kubota, M., Wada, M., Shimizu, R., Hoshi, S., Kozutsumi, H., Takasaki, S., & Kobata, A. (1989). Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.20.7819
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