The DOI matches its Crossref record.
Epstein-Barr virus nuclear protein 2 is a key determinant of lymphocyte transformation.
Open accessAbstract
Epstein-Barr virus (EBV) efficiently transforms B lymphocytes to perpetual proliferation. The EBV laboratory strain P3HR-1 is transformation-incompetent and lacks a DNA segment that includes the EBV nuclear antigen 2 (EBNA-2) gene and a portion of the EBNA leader protein (EBNA-LP) gene. These two genes are expressed in transformed B lymphocytes. Recombinant transformation-competent EBVs were produced by transfecting P3HR-1-infected cells with a cosmid containing the DNA deleted in P3HR-1. Deletion of 105 nucleotides from the middle of the EBNA-2 gene had no discernible affect on transformation. Two larger EBNA-2 deletions abolished transformation but did not affect EBNA-2 nuclear localization. Two naturally occurring EBV variants (EBV types 1 and 2) differ extensively in their growth-transformation phenotype and in their EBNA-LP, EBNA-2, and EBNA-3A, -3B, and -3C genes. Recombinant P3HR-1 carrying EBV-1 EBNA-2 has many of the EBV-1 in vitro growth-transforming effects; recombinant P3HR-1, isogenic except for EBV-2 EBNA-2, has many of the EBV-2 growth-transforming effects including slow emergence of transformants, growth in tight clumps with few surrounding viable cells, and early sensitivity to dilution with fresh medium. Thus, EBNA-2 is an essential molecule in lymphocyte growth transformation by EBV and a major determinant of the differences between EBV-1 and EBV-2 in lymphocyte growth transformation.
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Citations
Cited by
Mediation of Epstein-Barr Virus EBNA2 Transactivation by Recombination Signal-Binding Protein J κ
Henkel, T., Ling, P. D., Hayward, S. D., & Peterson, M. G. (1994) · Science · doi:10.1126/science.8016657 · Cited by 455
Hsieh, J. J.-D., Henkel, T., Salmon, P., Robey, E., Peterson, M. G., & Hayward, S. D. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.3.952 · Cited by 451
Epstein–Barr virus latent genes
Kang, M.-S., & Kieff, E. (2015) · Experimental & Molecular Medicine · doi:10.1038/emm.2014.84 · Cited by 361
Self-citationThe Proto-Oncogene c- myc Is a Direct Target Gene of Epstein-Barr Virus Nuclear Antigen 2
Kaiser, C., Laux, G., Eick, D., Jochner, N., Bornkamm, G. W., & Kempkes, B. (1999) · Journal of Virology · doi:10.1128/jvi.73.5.4481-4484.1999 · Cited by 273
Kaymaz, Y., Oduor, C. I., Yu, H., Otieno, J. A., Ong'echa, J. M., Moormann, A. M., & Bailey, J. A. (2017) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-16-0305 · Cited by 120
Metadata
- Authors
- Cohen, J. I., Wang, F., Mannick, J., & Kieff, E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-12
- Volume
- 86
- Issue
- 23
- Pages
- 9558-9562
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1985082049
- Cited by
- 483
- References
- 0
- FWCI
- 8.16
- Type
- journal article
- Language
- en
- Open access
- green
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- Cohen, J. I., Wang, F., Mannick, J., & Kieff, E. (1989). Epstein-Barr virus nuclear protein 2 is a key determinant of lymphocyte transformation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.23.9558
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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