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Molecular cloning and sequencing of cDNAs encoding the entire rat fatty acid synthase.
Open accessAbstract
Overlapping cloned cDNAs representing the entire sequence of the rat fatty acid synthase mRNA have been isolated from a cDNA library and sequenced. Authenticity of the cDNA clones was supported by hybridization to fatty acid synthase mRNA and by amino-terminal sequencing of 39 fatty acid synthase CNBr fragments. The full-length fatty acid synthase mRNA is 9156 nucleotides long and includes an 84-nucleotide 5' noncoding region, a 7515-nucleotide coding sequence, and a 1537-nucleotide 3' noncoding region; a second mRNA species containing a shortened 3' noncoding sequence is also transcribed in the rat. The encoded fatty acid synthase subunit contains 2505 amino acids and has a molecular weight of 272,340. Active sites and substrate binding sites were located within the sequence, thus establishing the order of domains on the multifunctional animal fatty acid synthase as condensing enzyme-transferase-dehydrase-enoyl reductase-ketoreductase-acyl carrier protein-thioesterase.
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Cited by
An analysis of vertebrate mRNA sequences: intimations of translational control.
Kozak, M. (1991) · The Journal of cell biology · doi:10.1083/jcb.115.4.887 · Cited by 1,635
Fatty acid synthesis: a potential selective target for antineoplastic therapy
Kuhajda, F. P., Jenner, K., Wood, F. D., Hennigar, R. A., Jacobs, L. B., Dick, J. D., & Pasternack, G. R. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.14.6379 · Cited by 705
Genetic Contributions to Understanding Polyketide Synthases
Hopwood, D. A. (1997) · Chemical Reviews · doi:10.1021/cr960034i · Cited by 710
BECK, J., RIPKA, S., SIEGNER, A., SCHILTZ, E., & SCHWEIZER, E. (1990) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1990.tb19252.x · Cited by 302
Tsay, J. T., Oh, W., Larson, T. J., Jackowski, S., & Rock, C. O. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50498-7 · Cited by 280
Metadata
- Authors
- Amy, C. M., Witkowski, A., Naggert, J., Williams, B., Randhawa, Z., & Smith, S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-05
- Volume
- 86
- Issue
- 9
- Pages
- 3114-3118
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2020587158
- Cited by
- 161
- References
- 0
- FWCI
- 6.21
- Type
- journal article
- Language
- en
- Open access
- green
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- Amy, C. M., Witkowski, A., Naggert, J., Williams, B., Randhawa, Z., & Smith, S. (1989). Molecular cloning and sequencing of cDNAs encoding the entire rat fatty acid synthase. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.9.3114
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