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Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis.
Open accessAbstract
Catabolite repression of the Bacillus subtilis alpha-amylase gene (amyE) involves an operator sequence located just downstream of the promoter (amyR), overlapping the transcription start site. Oligonucleotide site-directed mutagenesis of this sequence identified bases required for catabolite repression. Two mutations increased both the 2-fold symmetry of the operator and the repression ratio. Although many mutations reduced the repression ratio 3- to 11-fold, some also caused a 2-fold or greater increase in amylase production. Others caused hyperproduction without affecting catabolite repression. Homologous sequences in other catabolite-repressed B. subtilis promoters suggest a common regulatory site may be involved in catabolite repression.
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Citations
Cited by
Deutscher, J., Francke, C., & Postma, P. W. (2006) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00024-06 · Cited by 1,424
Wolfe, A. J. (2005) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.69.1.12-50.2005 · Cited by 1,233
Acetoin Metabolism in Bacteria
Xiao, Z., & Xu, P. (2007) · Critical Reviews in Microbiology · doi:10.1080/10408410701364604 · Cited by 375
Regulation of Carbon Catabolism in Bacillus Species
Stülke, J., & Hillen, W. (2000) · Annual Review of Microbiology · doi:10.1146/annurev.micro.54.1.849 · Cited by 370
A family of bacterial regulators homologous to Gal and Lac repressors.
Weickert, M. J., & Adhya, S. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)49615-4 · Cited by 364
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Metadata
- Authors
- Weickert, M. J., & Chambliss, G. H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1990-08
- Volume
- 87
- Issue
- 16
- Pages
- 6238-6242
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2049104376
- Cited by
- 293
- References
- 0
- FWCI
- 7.56
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis. https://app.cochat.ai/papers/10.1073/pnas.87.16.6238
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- Weickert, M. J., & Chambliss, G. H. (1990). Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.87.16.6238
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