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Stable transformation of plastids in higher plants.
Open accessAbstract
Stable genetic transformation of the plastid genome is reported in a higher plant, Nicotiana tabacum. Plastid transformation was obtained after bombardment of leaves with tungsten particles coated with pZS148 plasmid DNA. Plasmid pZS148 (9.6 kilobases) contains a 3.7-kilobase plastid DNA fragment encoding the 16S rRNA. In the 16S rRNA-encoding DNA (rDNA) a spectinomycin resistance mutation is flanked on the 5' side by a streptomycin resistance mutation and on the 3' side by a Pst I site generated by ligating an oligonucleotide in the intergenic region. Transgenic lines were selected by spectinomycin resistance and distinguished from spontaneous mutants by the flanking, cotransformed streptomycin resistance and Pst I markers. Regenerated plants are homoplasmic for the spectinomycin resistance and the Pst I markers and heteroplasmic for the unselected streptomycin resistance trait. Transgenic plastid traits are transmitted to the seed progeny. The transgenic plastid genomes are products of a multistep process, involving DNA recombination, copy correction, and sorting out of plastid DNA copies.
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Citations
Cited by
Recombination and the maintenance of plant organelle genome stability
Maréchal, A., & Brisson, N. (2010) · New Phytologist · doi:10.1111/j.1469-8137.2010.03195.x · Cited by 533
PLASTID TRANSFORMATION IN HIGHER PLANTS
Maliga, P. (2004) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.55.031903.141633 · Cited by 486
Self-citationParticle bombardment and the genetic enhancement of crops: myths and realities
Altpeter, F., Baisakh, N., Beachy, R., Bock, R., Capell, T., Christou, P., Daniell, H., Datta, K., Datta, S., Dix, P. J., et al. (2005) · Molecular Breeding · doi:10.1007/s11032-004-8001-y · Cited by 371
Elimination of deleterious mutations in plastid genomes by gene conversion
Khakhlova, O., & Bock, R. (2006) · The Plant Journal · doi:10.1111/j.1365-313x.2006.02673.x · Cited by 369
Chlamydomonas reinhardtii chloroplasts as protein factories
Mayfield, S. P., Manuell, A. L., Chen, S., Wu, J., Tran, M., Siefker, D., Muto, M., & Marin-Navarro, J. (2007) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2007.02.001 · Cited by 201
Metadata
- Authors
- Svab, Z., Hajdukiewicz, P., & Maliga, P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1990-11
- Volume
- 87
- Issue
- 21
- Pages
- 8526-8530
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2032379550
- Cited by
- 673
- References
- 0
- FWCI
- 22.16
- Type
- journal article
- Language
- en
- Open access
- green
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- Svab, Z., Hajdukiewicz, P., & Maliga, P. (1990). Stable transformation of plastids in higher plants. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.87.21.8526
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