Chlamydomonas reinhardtii chloroplasts as protein factories

Chloroplast Transformation in Chlamydomonas with High Velocity Microprojectiles

Boynton, J. E., Gillham, N. W., Harris, E. H., Hosler, J. P., Johnson, A. M., Jones, A. R., Randolph-Anderson, B. L., Robertson, D., Klein, T. M., Shark, K. B., et al. (1988) · Science · doi:10.1126/science.2897716 · Cited by 963 · see citing works

VITAMIN SYNTHESIS IN PLANTS: Tocopherols and Carotenoids

DellaPenna, D., & Pogson, B. J. (2006) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.56.032604.144301 · Cited by 838 · see citing works

Stable transformation of plastids in higher plants.

Sváb, Z., Hajdukiewicz, P. T. J., & Maliga, P. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.21.8526 · Cited by 675 · see citing works

Hydrogen Production. Green Algae as a Source of Energy

Melis, A., & Happe, T. (2001) · Plant Physiology · doi:10.1104/pp.010498 · Cited by 655 · see citing works

High-yield production of a human therapeutic protein in tobacco chloroplasts

Staub, J. M., Garcia, B. H., Graves, J., Hajdukiewicz, P. T. J., Hunter, P., Nehra, N. S., Paradkar, V. M., Schlittler, M. R., Carroll, J. A., Spatola, L. A., et al. (2000) · Nature Biotechnology · doi:10.1038/73796 · Cited by 486 · see citing works

Transgenic expression of aminoglycoside adenine transferase in the chloroplast: a selectable marker for site-directed transformation of chlamydomonas

Goldschmidt‐Clermont, M. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.15.4083 · Cited by 473 · see citing works

Mutations that Allow Disulfide Bond Formation in the Cytoplasm of Escherichia coli

Derman, A. I., Prinz, W. A., Belin, D., & Beckwith, J. (1993) · Science · doi:10.1126/science.8259521 · Cited by 431 · see citing works

Expression and assembly of a fully active antibody in algae

Mayfield, S. P., Franklin, S. E., & Lerner, R. A. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0237108100 · Cited by 345 · see citing works

Light-Regulated Translation of Chloroplast Messenger RNAs Through Redox Potential

Danon, A., & Mayfield, S. P. (1994) · Science · doi:10.1126/science.7992056 · Cited by 330 · see citing works

Effect of glycosylation on antibody function: implications for genetic engineering

Wright, A. M., & Morrison, S. L. (1997) · Trends in biotechnology · doi:10.1016/s0167-7799(96)10062-7 · Cited by 315 · see citing works

Foreign protein degradation and instability in plants and plant tissue cultures

Doran, P. M. (2006) · Trends in biotechnology · doi:10.1016/j.tibtech.2006.06.012 · Cited by 260 · see citing works

Condon usage tabulated from the international DNA sequence databases

Nakamura, Y. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.1.214 · Cited by 254 · see citing works

Codon Usage in Higher Plants, Green Algae, and Cyanobacteria

Campbell, W. H., & Gowri, G. (1990) · PLANT PHYSIOLOGY · doi:10.1104/pp.92.1.1 · Cited by 249 · see citing works

Plastid biotechnology: prospects for herbicide and insect resistance, metabolic engineering and molecular farming

Bock, R. (2006) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2006.12.001 · Cited by 248 · see citing works

Protein Disulfide Isomerase as a Regulator of Chloroplast Translational Activation

Kim, J., & Mayfield, S. P. (1997) · Science · doi:10.1126/science.278.5345.1954 · Cited by 238 · see citing works

Searching limiting steps in the expression of chloroplast‐encoded proteins: relations between gene copy number, transcription, transcript abundance and translation rate in the chloroplast of Chlamydomonas reinhardtii

Eberhard, S., Drapier, D., & Wollman, F.-A. (2002) · The Plant Journal · doi:10.1046/j.1365-313x.2002.01340.x · Cited by 227 · see citing works

Light regulated translational activators: identification of chloroplast gene specific mRNA binding proteins.

Danon, A., & Mayfield, S. P. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb04974.x · Cited by 214 · see citing works

When Transgenes Wander, Should We Worry?

Ellstrand, N. C. (2001) · Plant Physiology · doi:10.1104/pp.125.4.1543 · Cited by 205 · see citing works

Development of a GFP reporter gene for Chlamydomonas reinhardtii chloroplast

Franklin, S. E., Ngo, B. X., Efuet, E. T., & Mayfield, S. P. (2002) · The Plant Journal · doi:10.1046/j.1365-313x.2002.01319.x · Cited by 194 · see citing works

Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast

Sun, M., Qian, K., Su, N., Chang, H., Liu, J., & Shen, G. (2003) · Biotechnology Letters · doi:10.1023/a:1024140114505 · Cited by 185 · see citing works

Studies on Chlamydomonas chloroplast transformation: foreign DNA can be stably maintained in the chromosome.

Blowers, A. D., Bogorad, L., Shark, K. B., & Sanford, J. C. (1989) · The Plant Cell · doi:10.1105/tpc.1.1.123 · Cited by 160 · see citing works

Robust expression of a bioactive mammalian protein in <i>Chlamydomonas</i> chloroplast

Manuell, A. L., Beligni, M. V., Elder, J. H., Siefker, D. T., Tran, M., Weber, A., McDonald, T. L., & Mayfield, S. P. (2007) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2007.00249.x · Cited by 154 · see citing works

Chloroplast-derived vaccine antigens and other therapeutic proteins

Daniell, H., Chebolu, S., Kumar, S. B., et al. (2004) · Vaccine · doi:10.1016/j.vaccine.2004.11.004 · Cited by 152 · see citing works

ADP‐dependent phosphorylation regulates RNA‐binding in vitro: implications in light‐modulated translation.

Danon, A., & Mayfield, S. P. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06500.x · Cited by 150 · see citing works

Determinants for stability of the chloroplast psbD RNA are located within its short leader region in Chlamydomonas reinhardtii.

Nickelsen, J., Dillewijn, J. V., Rahire, M., et al. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06617.x · Cited by 149 · see citing works

Chloroplast Biogenesis of Photosystem II Cores Involves a Series of Assembly-Controlled Steps That Regulate Translation

Minai, L., Wostrikoff, K., Wollman, F.-A., & Choquet, Y. (2005) · The Plant Cell · doi:10.1105/tpc.105.037705 · Cited by 141 · see citing works

Tools for chloroplast transformation in Chlamydomonas: expression vectors and a new dominant selectable marker

Bateman, J. E., & Purton, S. (2000) · Molecular and General Genetics MGG · doi:10.1007/s004380051184 · Cited by 123 · see citing works

Inducible Trans-activation of Plastid Transgenes: Expression of the R. eutrophaphb Operon in Transplastomic Tobacco

Lössl, A. G., Bohmert, K., Harloff, H., Eibl, C., Mühlbauer, S. K., & Koop, H. (2005) · Plant and Cell Physiology · doi:10.1093/pcp/pci157 · Cited by 123 · see citing works

Comparative analysis of the biogenesis of photosystem II in the wild-type and Y-1 mutant of Chlamydomonas reinhardtii.

Malnoë, P., Mayfield, S. P., & Rochaix, J. (1988) · The Journal of Cell Biology · doi:10.1083/jcb.106.3.609 · Cited by 122 · see citing works

Stability determinants in the chloroplast psbB/T/H mRNAs of Chlamydomonas reinhardtii

Vaistij, F. E., Goldschmidt‐Clermont, M., Wostrikoff, K., et al. (2000) · The Plant Journal · doi:10.1046/j.1365-313x.2000.00700.x · Cited by 116 · see citing works

Expression of human antibodies in eukaryotic micro-algae

Mayfield, S. P., & Franklin, S. E. (2004) · Vaccine · doi:10.1016/j.vaccine.2004.11.013 · Cited by 115 · see citing works

In vivo evidence for 5′→3′ exoribonuclease degradation of an unstable chloroplast mRNA

Drager, R. G., Girard‐Bascou, J., Choquet, Y., et al. (1998) · The Plant Journal · doi:10.1046/j.1365-313x.1998.00016.x · Cited by 114 · see citing works

Translation of chloroplast psbA mRNA is regulated by signals initiated by both photosystems II and I

Trebitsh, T., & Danon, A. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.211440698 · Cited by 108 · see citing works

Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes

Barnes, D., Franklin, S. E., Schultz, J., Henry, R. E., Brown, E. C., Coragliotti, A., & Mayfield, S. P. (2005) · Molecular Genetics and Genomics · doi:10.1007/s00438-005-0055-y · Cited by 105 · see citing works

Transcriptional analysis of endogenous and foreign genes in chloroplast transformants of Chlamydomonas.

Blowers, A. D., Ellmore, G. S., Klein, U. K. A., & Bogorad, L. (1990) · The Plant Cell · doi:10.1105/tpc.2.11.1059 · Cited by 102 · see citing works

Codon usage tabulated from the international DNA sequence databases; its status 1999

Nakamura, Y., Gojobori, T., & Ikemura, T. (1999) · Nucleic Acids Research · doi:10.1093/nar/27.1.292 · Cited by 101 · see citing works

Uncorking the biomanufacturing bottleneck

Dove, A. (2002) · Nature Biotechnology · doi:10.1038/nbt0802-777 · Cited by 96 · see citing works

Development of a luciferase reporter gene, luxCt, for Chlamydomonas reinhardtii chloroplast

Mayfield, S. P., & Schultz, J. (2004) · The Plant Journal · doi:10.1046/j.1365-313x.2003.01965.x · Cited by 95 · see citing works

Two types of chloroplast gene promoters in Chlamydomonas reinhardtii.

Klein, U. K. A., Camp, J. D. D., & Bogorad, L. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.8.3453 · Cited by 83 · see citing works

Chloroplast RNA-binding proteins

Nickelsen, J. (2003) · Current Genetics · doi:10.1007/s00294-003-0425-0 · Cited by 79 · see citing works

Real-Time Monitoring of Chloroplast Gene Expression by a Luciferase Reporter: Evidence for Nuclear Regulation of Chloroplast Circadian Period

Matsuo, T., Onai, K., Okamoto, K., Minagawa, J., & Ishiura, M. (2006) · Molecular and Cellular Biology · doi:10.1128/mcb.26.3.863-870.2006 · Cited by 68 · see citing works

Expression of a foreign gene in Chlamydomonas reinhardtii chloroplast

Ishikura, K., Takaoka, Y., Kato, K., Sekine, M., Yoshida, K., & Shinmyō, A. (1999) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(99)80037-1 · Cited by 67 · see citing works

External control of transgene expression in tobacco plastids using the bacterial lac repressor

Mühlbauer, S. K., & Koop, H. (2005) · The Plant Journal · doi:10.1111/j.1365-313x.2005.02495.x · Cited by 66 · see citing works

Renilla luciferase as a vital reporter for chloroplast gene expression in Chlamydomonas

Minko, I. G., Holloway, S. P., Nikaido, S. S., Carter, M., ODOM, O. W., Johnson, C. H., & Herrin, D. L. (1999) · Molecular and General Genetics MGG · doi:10.1007/s004380051101 · Cited by 64 · see citing works

Chloroplast RNA processing and stability

Herrin, D. L., & Nickelsen, J. (2004) · Photosynthesis Research · doi:10.1007/s11120-004-2741-8 · Cited by 62 · see citing works

T7 RNA Polymerase-Directed Expression of an Antibody Fragment Transgene in Plastids Causes a Semi-Lethal Pale-Green Seedling Phenotype

Magee, A. M., Coyne, S., Murphy, D., et al. (2004) · Transgenic Research · doi:10.1023/b:trag.0000040019.35147.a4 · Cited by 61 · see citing works

Activity of the Chlamydomonas chloroplast rbcL gene promoter is enhanced by a remote sequence element.

Klein, U. K. A., Salvador, M. L., & Bogorad, L. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.23.10819 · Cited by 56 · see citing works

Specific roles of 5' RNA secondary structures in stabilizing transcripts in chloroplasts

Suay, L. S., Salvador, M. L., Abesha, E., et al. (2005) · Nucleic Acids Research · doi:10.1093/nar/gki760 · Cited by 54 · see citing works

Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast

Yang, Z., Li, Y., Chen, F., Li, D., Zhang, Z., Liu, Y., Zheng, D., Wang, Y., & Shen, G. (2006) · Chinese Science Bulletin · doi:10.1007/s11434-006-2041-0 · Cited by 53 · see citing works

Changes in the 5′-untranslated region of the rbcL gene accelerate transcript degradation more than 50-fold in the chloroplast of Chlamydomonas reinhardtii

Salvador, M. L., Suay, L. S., Anthonisen, I. L., et al. (2003) · Current Genetics · doi:10.1007/s00294-003-0470-8 · Cited by 41 · see citing works

Recombination and Heterologous Expression of Allophycocyanin Gene in the Chloroplast of Chlamydomonas reinhardtii

Su, Z., Qian, K., Tan, C.-P., Meng, C., & Qin, S. (2005) · Acta Biochimica et Biophysica Sinica · doi:10.1111/j.1745-7270.2005.00092.x · Cited by 36 · see citing works

Hybrid transcription system for controlled plastid transgene expression

Tomlinson, L., Horváth, É., Medgyesy, P., et al. (2006) · The Plant Journal · doi:10.1111/j.1365-313x.2006.02718.x · Cited by 35 · see citing works

Survival of Human Metallothionein-2 Transplastomic Chlamydomonas reinhardtii to Ultraviolet B Exposure

Zhang, Y., Shen, G., & Ru, B. (2006) · Acta Biochimica et Biophysica Sinica · doi:10.1111/j.1745-7270.2006.00148.x · Cited by 34 · see citing works

Biofuels from algae: Challenges and potential

Hannon, M., Gimpel, J., Tran, M., Rasala, B., & Mayfield, S. (2010) · Biofuels · doi:10.4155/bfs.10.44 · Cited by 813 · see citing works

Microalgae as a future food source

Torres-Tiji, Y., Fields, F. J., & Mayfield, S. P. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107536 · Cited by 559 · see citing works

The microalga<i>Chlamydomonas reinhardtii</i>as a platform for the production of human protein therapeutics

Rasala, B. A., & Mayfield, S. P. (2011) · Bioengineered Bugs · doi:10.4161/bbug.2.1.13423 · Cited by 122 · see citing works

Genetic engineering of algal chloroplasts: progress and prospects

Purton, S., Szaub, J. B., Wannathong, T., Young, R., & Economou, C. K. (2013) · Russian Journal of Plant Physiology · doi:10.1134/s1021443713040146 · Cited by 74 · see citing works

System and method for research-scale outdoor production of microalgae and cyanobacteria

Schoepp, N. G., Stewart, R. L., Sun, V., Quigley, A. J., Mendola, D., Mayfield, S. P., & Burkart, M. D. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.05.046 · Cited by 68 · see citing works

Chlamydomonas reinhardtii: Biological Rationale for Genomics1

Dutcher, S. K. (2000) · Journal of Eukaryotic Microbiology · doi:10.1111/j.1550-7408.2000.tb00059.x · Cited by 18 · see citing works

Μελέτη της βιοαποικοδόμησης φαινολικών ενώσεων από μικροοργανισμούς

Νάζος, Θ. (2018) · doi:10.12681/eadd/44174

A marineChlamydomonassp. emerging as an algal model

Flores, D. C., Fricke, M., Wesp, V., Desirò, D., Kniewasser, A., Hölzer, M., Marz, M., & Mittag, M. (2020) · Journal of Phycology · doi:10.1111/jpy.13083 · Cited by 11 · see citing works

Draft Genome Sequences of Four Species of Chlamydomonas Containing Phosphatidylcholine

Hirashima, T., Tajima, N., & Sato, N. (2016) · Genome Announcements · doi:10.1128/genomea.01070-16 · Cited by 19 · see citing works

Proteomic Analysis of Hydrogen Photoproduction in Sulfur-Deprived Chlamydomonas Cells

Chen, M., Zhao, L., Sun, Y.-L., Cui, S., Zhang, L., Yang, B., Wang, J., Kuang, T., & Huang, F. L. (2010) · Journal of Proteome Research · doi:10.1021/pr100076c · Cited by 57 · see citing works