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Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor.
Open accessAbstract
Cytotoxic lymphocyte maturation factor (CLMF) is a disulfide-bonded heterodimeric lymphokine that (i) acts as a growth factor for activated T cells independent of interleukin 2 and (ii) synergizes with suboptimal concentrations of interleukin 2 to induce lymphokine-activated killer cells. We now report the cloning and expression of both human CLMF subunit cDNAs from a lymphoblastoid B-cell line, NC-37. The two subunits represent two distinct and unrelated gene products whose mRNAs are coordinately induced upon activation of NC-37 cells. Coexpression of the two subunit cDNAs in COS cells is necessary for the secretion of biologically active CLMF; COS cells transfected with either subunit cDNA alone do not secrete bioactive CLMF. Recombinant CLMF expressed in mammalian cells displays biologic activities essentially identical to natural CLMF, and its activities can be neutralized by monoclonal antibodies prepared against natural CLMF. Since this heterodimeric protein displays the properties of an interleukin, we propose that CLMF be given the designation interleukin 12.
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Citations
Cited by
Interleukin-12: A Cytokine at the Interface of Inflammation and Immunity
Trinchieri, G. (1998) · Advances in Immunology · doi:10.1016/s0065-2776(08)60387-9 · Cited by 760
Xanthoudakis, S., & Curran, T. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05097.x · Cited by 708
Trembleau, S., Penna, G., Bosi, E., Mortara, A., Gately, M. K., & Adorini, L. (1995) · The Journal of experimental medicine · doi:10.1084/jem.181.2.817 · Cited by 423
D'Andrea, A., Ma, X., Aste-Amezaga, M., Paganin, C., & Trinchieri, G. (1995) · The Journal of experimental medicine · doi:10.1084/jem.181.2.537 · Cited by 365
Interferon-γ and interleukin-12 pathway defects and human disease
Dorman, S. E., & Holland, S. M. (2000) · Cytokine & Growth Factor Reviews · doi:10.1016/s1359-6101(00)00010-1 · Cited by 336
Metadata
- Authors
- Gubler, U., Chua, A. O., Schoenhaut, D. S., Dwyer, C. M., McComas, W., Motyka, R., Nabavi, N., Wolitzky, A. G., Quinn, P. M., & Familletti, P. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1991-05-15
- Volume
- 88
- Issue
- 10
- Pages
- 4143-4147
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1977817262
- Cited by
- 576
- References
- 0
- FWCI
- 8.23
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor. https://app.cochat.ai/papers/10.1073/pnas.88.10.4143
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- Gubler, U., Chua, A. O., Schoenhaut, D. S., Dwyer, C. M., McComas, W., Motyka, R., Nabavi, N., Wolitzky, A. G., Quinn, P. M., & Familletti, P. C. (1991). Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.88.10.4143
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
