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Saccharide orientation at the cell surface affects glycolipid receptor function.
Open accessAbstract
Three allelic variants of P-pilus-associated G-adhesins (lectins) with different cell-binding properties were recently described. Here we have analyzed Escherichia coli HB101 strains expressing the recombinant G-adhesin variants for their ability to agglutinate erythrocytes from various species as this relates to the glycosphingolipid (GSL) composition in the erythrocyte membranes. All three variants exhibit similar specificities for the globo-series GSLs affixed to artificial surfaces. However, only the PapGJ96 adhesin induces agglutination of erythrocytes having globotriaosylceramide (GbO3) [Gal(alpha 1-4)LacCer] as the major GSL. Furthermore, only PapGAD110 induces strong agglutination of erythrocytes having globotetraosylceramide (GbO4) [GalNAc(beta 1-3)Gal(alpha 1-4)LacCer] as the major GSL, while PrsGJ96 alone agglutinates those containing globopentaosylceramide (GbO5) [GalNAc(alpha 1-3)GalNAc(beta 1-3)Gal(alpha 1-4)LacCer]. Molecular modeling of these globo-GSLs demonstrates different saccharide orientations to the membrane surface for these isoreceptors. We suggest that the differential binding of the three G-adhesin variants results from differences in epitope presentation at the membrane among these globo-GSLs.
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Cited by
Pilus and nonpilus bacterial adhesins: Assembly and function in cell recognition
Hultgren, S. J., Abraham, S., Caparon, M., Falk, P., Geme, J. W. S., & Normark, S. (1993) · Cell · doi:10.1016/0092-8674(93)90269-v · Cited by 409
Self-citationWu, X. R., Sun, T. T., & Medina, J. J. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.18.9630 · Cited by 342
Kiarash, A., Boyd, B., & Lingwood, C. A. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)78102-2 · Cited by 206
Metadata
- Authors
- Strömberg, N., Nyholm, P. G., Pascher, I., & Normark, S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1991-10-15
- Volume
- 88
- Issue
- 20
- Pages
- 9340-9344
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2024152313
- Cited by
- 197
- References
- 0
- FWCI
- 8.08
- Type
- journal article
- Language
- en
- Open access
- green
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- Strömberg, N., Nyholm, P. G., Pascher, I., & Normark, S. (1991). Saccharide orientation at the cell surface affects glycolipid receptor function. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.88.20.9340
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