The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Sequence requirement for peptide recognition by rat brain p21ras protein farnesyltransferase.
Open accessAbstract
We tested 42 tetrapeptides for their ability to bind to the rat brain p21ras protein farnesyltransferase as estimated by their ability to compete with p21Ha-ras in a farnesyltransfer assay. Peptides with the highest affinity had the structure Cys-A1-A2-X, where positions A1 and A2 are occupied by aliphatic amino acids and position X is occupied by a COOH-terminal methionine, serine, or phenylalanine. Charged residues reduced affinity slightly at the A1 position and much more drastically at the A2 and X positions. Effective inhibitors included tetrapeptides corresponding to the COOH termini of all animal cell proteins known to be farnesylated. In contrast, the tetrapeptide Cys-Ala-Ile-Leu (CAIL), which corresponds to the COOH termini of several neural guanine nucleotide binding (G) protein gamma subunits, did not compete in the farnesyl-transfer assay. Inasmuch as several of these proteins are geranylgeranylated, the data suggest that the two isoprenes (farnesyl and geranylgeranyl) are transferred by different enzymes. A biotinylated heptapeptide corresponding to the COOH terminus of p21Ki-rasB was farnesylated, suggesting that at least some of the peptides serve as substrates for the transferase. The data are consistent with a model in which a hydrophobic pocket in the protein farnesyltransferase recognizes tetrapeptides through interactions with the cysteine and the last two amino acids.
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Cites
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K- and N-Ras Are Geranylgeranylated in Cells Treated with Farnesyl Protein Transferase Inhibitors
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A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles
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PROTEIN PRENYLATION: GENES, ENZYMES, TARGETS, AND FUNCTIONS
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Direct Demonstration of Geranylgeranylation and Farnesylation of Ki-Ras in Vivo
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Metadata
- Authors
- Reiss, Y., Stradley, S. J., Gierasch, L. M., Brown, M. S., & Goldstein, J. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1991-02
- Volume
- 88
- Issue
- 3
- Pages
- 732-736
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2060264934
- Cited by
- 313
- References
- 23
- FWCI
- 12.30
- Type
- journal article
- Language
- en
- Open access
- green
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- Reiss, Y., Stradley, S. J., Gierasch, L. M., Brown, M. S., & Goldstein, J. L. (1991). Sequence requirement for peptide recognition by rat brain p21ras protein farnesyltransferase. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.88.3.732
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