The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Construction of a uniform-abundance (normalized) cDNA library.
Open accessAbstract
We have used a kinetic approach to construct cDNA libraries containing approximately equal representations of all sequences in a preparation of poly(A)+ RNA. Randomly primed cDNA fragments of a selected size range were cloned in lambda phage vector. Inserts were amplified by the polymerase chain reaction (PCR), denatured, and self-annealed under optimized conditions. After extensive but incomplete reannealing, the single-stranded fraction was relatively depleted of more abundant species of cDNA. Libraries of these fragments are suitable for cDNA subtraction, screening, or selection by hybridization and make it possible to detect and analyze cDNA corresponding to species of mRNA present at a low level in a small fraction of the cells in a complex tissue.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Cited by
Counting absolute numbers of molecules using unique molecular identifiers
Kivioja, T., Vähärautio, A., Karlsson, K., Bonke, M., Enge, M., Linnarsson, S., & Taipale, J. (2012) · Nature Methods · doi:10.1038/nmeth.1778 · Cited by 1,010
Cajigas, I. J., Tushev, G., Will, T. J., tom Dieck, S., Fuerst, N., & Schuman, E. M. (2012) · Neuron · doi:10.1016/j.neuron.2012.02.036 · Cited by 709
Newman, T., de Bruijn, F. J., Green, P., Keegstra, K., Kende, H., McIntosh, L., Ohlrogge, J., Raikhel, N., Somerville, S., Thomashow, M., et al. (1994) · Plant Physiology · doi:10.1104/pp.106.4.1241 · Cited by 632
Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression
Okubo, K., Hori, N., Matoba, R., Niiyama, T., Fukushima, A., Kojima, Y., & Matsubara, K. (1992) · Nature Genetics · doi:10.1038/ng1192-173 · Cited by 522
Lo, J., Lee, S., Xu, M., Liu, F., Ruan, H., Eun, A., He, Y., Ma, W., Wang, W., Wen, Z., et al. (2003) · Genome Research · doi:10.1101/gr.885403 · Cited by 265
Metadata
- Authors
- Patanjali, S. R., Parimoo, S., & Weissman, S. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1991-03
- Volume
- 88
- Issue
- 5
- Pages
- 1943-1947
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2026710989
- Cited by
- 167
- References
- 1
- FWCI
- 4.44
- Type
- journal article
- Language
- en
- Open access
- green
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- Patanjali, S. R., Parimoo, S., & Weissman, S. M. (1991). Construction of a uniform-abundance (normalized) cDNA library. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.88.5.1943
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