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Cited by 52Genomics and Chromatin DynamicsNuclear Structure and FunctionPlant Molecular Biology Research

Ribosomal gene clusters are uniquely proportioned between open and closed chromatin structures in both tomato leaf cells and exponentially growing suspension cultures

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Conconi, A, Sogo, J M, Ryan, C A·1992·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.89.12.5256PMC PMC49270
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Abstract

The accessibility of regulatory molecules to specific DNA sequences and chromatin regions in the nucleus is crucial to gene expression. In this study, we examined the chromatin structure in tomato leaf cells and in exponentially growing tomato cell suspension cultures. The structure of ribosomal chromatin was investigated by micrococcal nuclease and psoralen photocrosslinking. We showed that ribosomal genes in tomato are folded into two distinct types of chromatin: an open chromatin conformation and a closed nucleosomecontaining chromatin. In contrast to previous findings in Friend cells, where half of the ribosomal genes were found to be complexed within an inactive chromatin structure, we demonstrated that the canonical nucleosome-containing chromatin is present in the majority (approximately 80%) of the tomato rRNA-encoding DNA clusters. The minor open chromatin population (approximately 20% of the ribosomal genes) could be detected only after analysis following psoralen crosslinking. The relative amounts of the two ribosomal chromatin structures are similar in stationary and exponentially growing cells. This suggests that the proportions of open and closed chromatin structures present in either stationary or exponentially growing tomato cells are not dependent on the transcriptional process.

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  • Only a part of rDNA units is transcriptionally active at any time, while the rest, or even the majority of rDNA repeats, are in a repressed state.

    “Numerous genetic, microscopic and biochemical studies have demonstrated that only a part of rDNA units is transcriptionally active at any time, while the rest, or even the majority of rDNA repeats, are in a repressed state (6–14).”

    From Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon (2022) · cites this paper as [9]

    The indexed copy provides an abstract and references, but no article-body passage addressing how many rDNA units are transcriptionally active or repressed. I searched for the majority of tomato rDNA clusters being in closed chromatin; the matching statement appears only in the abstract, which cannot serve as evidence under this rubric. (LLM, conf=0.72)

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Research works printed but not linked: 28

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Cited by

Sorted by citations

Authors
Conconi, A., Sogo, J. M., & Ryan, C. A.
Affiliations
Washington State University (US)
ORCID
Conconi, A
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1992-06-15
Volume
89
Issue
12
Pages
5256-5260
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.89.12.5256
PMC
PMC49270
OpenAlex
W2071069279
Cited by
52
References
0
Citations by year
2025: 1 · 2023: 2 · 2021: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 2 · 2013: 1 · 2012: 3
FWCI
1.06
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords
chromatin structure, nucleosome, open chromatin, closed chromatin, ribosomal DNA, tomato, cell suspension culture, leaf cells, psoralen crosslinking, staphylococcal nuclease, gene expression regulation, cell growth phase

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