Two different chromatin structures coexist in ribosomal RNA genes throughout the cell cycle
Open accessAbstract
The structure of ribosomal chromatin in exponentially growing Friend cells, in stationary cells, and in metaphase chromosomes was studied by psoralen photocrosslinking. It is shown that in intact cells, two distinct types of ribosomal chromatin coexist in Friend cells, one that contains nucleosomes and represents the inactive copies and one that lacks a repeating structure and corresponds to the transcribed genes. A single gene copy is either in one or the other chromatin state. The relative amounts of the two types of structures are similar in interphase and metaphase, however, their run-on activities differ significantly. This suggests that the two states of chromatin are maintained independently of the transcriptional process and that they are stably propagated through the cell cycle.
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Only a part of rDNA units is transcriptionally active at any time, while the rest, or even the majority of rDNA repeats, are in a repressed state.
“Numerous genetic, microscopic and biochemical studies have demonstrated that only a part of rDNA units is transcriptionally active at any time, while the rest, or even the majority of rDNA repeats, are in a repressed state (6–14).”
From Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon (2022) · cites this paper as [8]
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- Authors
- Conconi, A., Widmer, R. M., Koller, T., & Sogo, J.
- Affiliations
- ETH Zurich (CH)
- ORCID
- Conconi, Antonio
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1989-06
- Volume
- 57
- Issue
- 5
- Pages
- 753-761
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2020805433
- Cited by
- 390
- References
- 0
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 10 · 2023: 11 · 2022: 13 · 2021: 13 · 2020: 11 · 2019: 19 · 2018: 9 · 2017: 11 · 2016: 13 · 2015: 9 · 2014: 6 · 2013: 13 · 2012: 15
- FWCI
- 3.48
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- ribosomal DNA, chromatin structure, nucleosome, cell cycle, metaphase chromosomes, transcription
- MeSH terms
- Animals, Cell Cycle, Cell Line, Cell Nucleus, Chromatin, Chromosomes, Cross-Linking Reagents, DNA, Ribosomal, Leukemia, Erythroblastic, Acute, Metaphase, RNA, Ribosomal, Transcription, Genetic, Trioxsalen, Mice
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