The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cytosolic phospholipase A2 is coupled to hormonally regulated release of arachidonic acid.
Open accessAbstract
Cytosolic phospholipase A2 (cPLA2) binds to natural membrane vesicles in a Ca(2+)-dependent fashion, resulting in the selective release of arachidonic acid, thus implicating cPLA2 in the hormonally regulated production of eicosanoids. Here we report that the treatment of Chinese hamster ovary (CHO) cells overexpressing cPLA2 with ATP or thrombin resulted in an increased release of arachidonic acid as compared with parental CHO cells, demonstrating the hormonal coupling of cPLA2. In contrast, CHO cells overexpressing a secreted form of mammalian PLA2 (sPLA2-II) failed to show any increased hormonal responsiveness. Interestingly, we have noted that the activation of cPLA2 with a wide variety of agents stimulates the phosphorylation of cPLA2 on serine residues. Pretreatment of cells with staurosporin blocked the ATP-mediated phosphorylation of cPLA2 and strongly inhibited the activation of the enzyme. Increased cPLA2 activity was also observed in lysates prepared from ATP-treated cells and was sensitive to phosphatase treatment. These results suggest that in addition to Ca2+, the phosphorylation of cPLA2 plays an important role in the agonist-induced activation of cPLA2.
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Cites
Clark, J. D., Lin, L.-L., Kriz, R. W., Ramesha, C. S., Sultzman, L. A., Lin, A. Y., Milona, N., & Knopf, J. L. (1991) · Cell · doi:10.1016/0092-8674(91)90556-e · Cited by 1,688
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Cited by
Nemenoff, R. A., Winitz, S., Qian, N. X., Van Putten, V., Johnson, G. L., & Heasley, L. E. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53948-x · Cited by 522
Interleukin-1 Enhances the ATP-Evoked Release of Arachidonic Acid from Mouse Astrocytes
Stella, N., Estellés, A., Siciliano, J., Tencé, M., Desagher, S., Piomelli, D., Glowinski, J., & Prémont, J. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-09-02939.1997 · Cited by 85
LRP1 Controls cPLA2 Phosphorylation, ABCA1 Expression and Cellular Cholesterol Export
Zhou, L., Choi, H. Y., Li, W.-P., Xu, F., & Herz, J. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006853 · Cited by 45
Wood, M. W., Segal, J. A., Mark, R. J., Ogden, A. M., & Felder, C. C. (2000) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2000.0742033.x · Cited by 11
Metadata
- Authors
- Lin, L. L., Lin, A. Y., & Knopf, J. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1992-07
- Volume
- 89
- Issue
- 13
- Pages
- 6147-6151
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2022037486
- Cited by
- 539
- References
- 1
- FWCI
- 33.67
- Type
- journal article
- Language
- en
- Open access
- green
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- Lin, L. L., Lin, A. Y., & Knopf, J. L. (1992). Cytosolic phospholipase A2 is coupled to hormonally regulated release of arachidonic acid. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.89.13.6147
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
