Direction of microtubule movement is an intrinsic property of the motor domains of kinesin heavy chain and Drosophila ncd protein.

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Smith, D. B., & Johnson, K. S. (1988) · Gene · doi:10.1016/0378-1119(88)90005-4 · Cited by 5,844

Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase.

Guan, K., & Dixon, J. (1991) · Analytical Biochemistry · doi:10.1016/0003-2697(91)90534-z · Cited by 1,735

Mechanochemical coupling in actomyosin energy transduction studied by in vitro movement assay

Harada, Y., Sakurada, K., Aoki, T., Thomas, D. D., & Yanagida, T. (1990) · Journal of Molecular Biology · doi:10.1016/s0022-2836(05)80060-9 · Cited by 522

A three-domain structure of kinesin heavy chain revealed by DNA sequence and microtubule binding analyses

Yang, J. T., Laymon, R. A., & Goldstein, L. S. B. (1989) · Cell · doi:10.1016/0092-8674(89)90692-2 · Cited by 395

The Drosophila claret segregation protein is a minus-end directed motor molecule

Walker, R. A., Salmon, E. D., & Endow, S. A. (1990) · Nature · doi:10.1038/347780a0 · Cited by 347

The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motor

McDonald, H. B., Stewart, R. J., & Goldstein, L. S. B. (1990) · Cell · doi:10.1016/0092-8674(90)90412-8 · Cited by 342

The Initial Phosphate Burst in ATP Hydrolysis by Myosin and Subfragment-1 as Studied by a Modified Malachite Green Method for Determination of Inorganic Phosphate

Kodama, T., Fukui, K., & Kometani, K. (1986) · The Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a135616 · Cited by 299

Mediation of meiotic and early mitotic chromosome segregation in Drosophila by a protein related to kinesin

Endow, S. A., Henikoff, S., & Soler-Niedziela, L. (1990) · Nature · doi:10.1038/345081a0 · Cited by 227

Evidence that the Head of Kinesin Is Sufficient for Force Generation and Motility in Vitro

Yang, J. T., Saxton, W. M., Stewart, R. J., Raff, E. C., & Goldstein, L. S. B. (1990) · Science · doi:10.1126/science.2142332 · Cited by 225

Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.

Ingold, A. L., Cohn, S. A., & Scholey, J. M. (1988) · The Journal of Cell Biology · doi:10.1083/jcb.107.6.2657 · Cited by 175

Contributions of molecular motor enzymes to vesicle-based protein transport in gastrointestinal epithelial cells

McNiven, M. A., & Marlowe, K. J. (1999) · Gastroenterology · doi:10.1016/s0016-5085(99)70142-3 · Cited by 29

Isopolar microtubule arrays as a tool to determine motor protein directionality

Prots, I., Stracke, R., Unger, E., & Böhm, K. J. (2003) · Cell Biology International · doi:10.1016/s1065-6995(02)00313-x · Cited by 27

Directional motility of kinesin motor proteins

Woehlke, G., & Schliwa, M. (2000) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/s0167-4889(00)00013-6 · Cited by 25

Motoring along the hyphae: molecular motors and the fungal cytoskeleton

Yamashita, R. A., & May, G. S. (1998) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(98)80088-4 · Cited by 22

A stop or go switch: glycogen synthase kinase 3β phosphorylation of the kinesin 1 motor domain at Ser314 halts motility without detaching from microtubules

Banerjee, R., Chakraborty, P., Yu, M. C., & Gunawardena, S. (2021) · Development · doi:10.1242/dev.199866 · Cited by 23

The ATPase Cross-bridge Cycle of the Kar3 Motor Domain

Mackey, A. T., & Gilbert, S. P. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m206219200 · Cited by 21

The mechanics of force generation by kinesin.

Howard, J. (1995) · Europe PMC (PubMed Central) · Cited by 21

A Developmentally Regulated Kinesin-related Motor Protein fromDictyostelium discoideum

de Hostos, E. L., McCaffrey, G., Sucgang, R., Pierce, D. W., & Vale, R. D. (1998) · Molecular Biology of the Cell · doi:10.1091/mbc.9.8.2093 · Cited by 17

Nucleotide-Dependent Interaction of the N-Terminal Domain of MukB with Microtubules

Lockhart, A., & Kendrick‐Jones, J. (1998) · Journal of Structural Biology · doi:10.1006/jsbi.1998.4056 · Cited by 17

Structural and functional features of one- and two-headed biotinated kinesin derivatives.

Gelles, J., Berliner, E., Young, E. C., Mahtani, H. K., Pérez-Ramírez, B., & Anderson, K. (1995) · Europe PMC (PubMed Central) · Cited by 15

Engineering a Lever into the Kinesin Neck

Mazumdar, M., & Cross, R. A. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.45.29352 · Cited by 15

Ratiometric ATP detection on gliding microtubules based on bioorthogonal fluorescence conjugation

Feng, G., Zhang, B., Wang, S., Ding, Z., Li, G., & Zhang, X. (2019) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2019.127090 · Cited by 13

Two heads are better than one

Schnapp, B. J. (1995) · Nature · doi:10.1038/373655a0 · Cited by 13

The shapes of the motor domains of two oppositely directed microtubule motors, ncd and kinesin: a neutron scattering study

Fujiwara, S., Kull, F. J., Sablin, E. P., Stone, D. B., & Mendelson, R. A. (1995) · Biophysical Journal · doi:10.1016/s0006-3495(95)80028-7 · Cited by 13

Automated Multi-Peak Tracking Kymography (AMTraK): A Tool to Quantify Sub-Cellular Dynamics with Sub-Pixel Accuracy

Chaphalkar, A. R., Jain, K., Gangan, M. S., & Athale, C. A. (2016) · PLoS ONE · doi:10.1371/journal.pone.0167620 · Cited by 11

4.7 Force Generation by Dynamic Microtubule Polymers

Grishchuk, E. L., McIntosh, J. R., Molodtsov, M. I., & Иноятович, А. Ф. (2012) · Elsevier eBooks · doi:10.1016/b978-0-12-374920-8.00409-4 · Cited by 9

N‐terminal β‐strand of single‐headed kinesin‐1 can modulate the off‐axis force‐generation and resultant rotation pitch

Yamagishi, M., Fujimura, S., Sugawa, M., Nishizaka, T., & Yajima, J. (2020) · Cytoskeleton · doi:10.1002/cm.21630 · Cited by 8

Structural features involved in force generation in the kinesin superfamily.

Goldstein, L. S. B. (1995) · Europe PMC (PubMed Central) · Cited by 8

In Vitro Assays to Study the Tracking of Shortening Microtubule Ends and to Measure Associated Forces

Grishchuk, E. L., & Ataullakhanov, F. I. (2010) · Methods in cell biology · doi:10.1016/s0091-679x(10)95033-4 · Cited by 7

Retroactive Motors

Schnapp, B. J. (1997) · Neuron · doi:10.1016/s0896-6273(00)80293-7 · Cited by 7

Microscopic approaches to dynamics and structure of biological motors

Gittes, F., & Schmidt, C. F. (1996) · Current Opinion in Solid State and Materials Science · doi:10.1016/s1359-0286(96)80034-9 · Cited by 6

Force generation by cellular motors.

Wanka, F., & van Zoelen, E. J. J. (2003) · PubMed · Cited by 6

Direction and speed of microtubule movements driven by kinesin motors arranged on catchin thick filaments

Taba, T., Edamatsu, M., Toba, S., Shibata, K., Imafuku, Y., Toyoshima, Y. Y., Tawada, K., & Yamada, A. (2008) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.20303 · Cited by 5

Kinesin heads fused to hinge‐free myosin tails drive efficient motility

Eickel, V., Drummond, D. R., Carter, N., Lockhart, A., Jones, J. K., & Cross, R. A. (2004) · FEBS Letters · doi:10.1016/j.febslet.2004.05.050 · Cited by 5

Kinesin and NCD, two structural cousins of myosin

Sellers, J. R. (1996) · Journal of Muscle Research and Cell Motility · doi:10.1007/bf00124239 · Cited by 5

Structural transitions in kinesin minus-end directed microtubule motilityPreprint

Shibata, S., Wang, M. Y., Imasaki, T., Shigematsu, H., Wei, Y., Jobichen, C., Hagio, H., Sivaraman, J., Endow, S. A., & Nitta, R. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.07.29.605428 · Cited by 6

Tether-scanning the kinesin motor domain reveals a core mechanical action

Sumiyoshi, R., Yamagishi, M., Furuta, A., Nishizaka, T., Furuta, K., Cross, R. A., & Yajima, J. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2403739121 · Cited by 4

Determinants of motor polarity in the kinesin proteins.

Endow, S. A. (1995) · Europe PMC (PubMed Central) · Cited by 4

The muscle in kinesin

Sakowicz, R., & Goldstein, L. S. B. (1996) · Nature Structural Biology · doi:10.1038/nsb0596-404 · Cited by 4

Assaying Spatial Organization of Microtubules by Kinesin Motors

Nédélec, F., & Surrey, T. (2001) · Humana Press eBooks · doi:10.1385/1-59259-069-1:213 · Cited by 3

The Kinesin-14 tail: Dual microtubule binding domains drive spindle morphogenesis through tight microtubule cross-linking and robust sliding

Ems-McClung, S. C., Cassity, M., Prasannajith, A., & Walczak, C. (2025) · Molecular Biology of the Cell · doi:10.1091/mbc.e25-02-0083 · Cited by 2

Structural analysis of a motor with increased mechanical output reveals new transitions in kinesin microtubule motility

Shibata, S., Wang, M. Y., Imasaki, T., Shigematsu, H., Torre, D. U. L., Wei, Y., Jobichen, C., Hagio, H., Sivaraman, J., Sugita, Y., et al. (2026) · Scientific Reports · doi:10.1038/s41598-025-28573-7 · Cited by 2

Local direction change of surface gliding microtubules

Li, F., Pan, J., & Choi, J. H. (2019) · Biotechnology and Bioengineering · doi:10.1002/bit.26933 · Cited by 1

Evidence for Motility Determinants in the Kinesin‐1 Minimal Motor Core Domain From Tether Variations

Sumiyoshi, R., Yamagishi, M., & Yajima, J. (2025) · Cytoskeleton · doi:10.1002/cm.70029 · Cited by 1

Engineering a Molecular Railroad

Stewart, R. J., & Limberis, L. (2008) · Humana Press eBooks · doi:10.1007/978-1-59745-218-2_18 · Cited by 1

Motor proteins in mitosis and meiosis

Yen, T. J. (1995) · The Cytoskeleton · doi:10.1016/s1874-6020(06)80006-6 · Cited by 1

Classification of single molecular motor events

Butterfield, A. E., & Skliar, M. (2004) · Proceedings of the ... American Control Conference/Proceedings of the American Control Conference · doi:10.1109/acc.2003.1239862 · Cited by 1

Polyene Cyclization to Adociasulfate 1

Lindel, T., & Hopmann, C. (2003) · Wiley eBooks · doi:10.1002/9783527619986.ch31 · Cited by 1

Crosslinking studies on the conventional kinesin of Neurospora crassa

Hahlen, K. (2004) · Elektronische Hochschulschriften der LMU München (Ludwig-Maximilians-Universität München) · doi:10.5282/edoc.2619

Polyene Cyclization to Adociasulfate 1

L'Mdel, T., & Hopmann, C. (2003) · Organic Synthesis Set · doi:10.1002/9783527620784.ch31d

High Resolution Two-Dimensional Detection of Ncd Motility with Optical Tweezers

Allersma, M. W., Gittes, F., deCastro, M. J., Stewart, R. J., & Schmidt, C. F. (1997) · MRS Proceedings · doi:10.1557/proc-489-85

Characterizing the cargo binding and regulatory function of the tail domain in Ncd motor protein

Lonergan, N. E. (2009) · VTechWorks (Virginia Tech)

In vitro motility-based tether-scanning of the kinesin motor domain

Sumiyoshi, R., Yamagishi, M., & Yajima, J. (2025) · MethodsX · doi:10.1016/j.mex.2025.103719

Mechanisms of Kinesin Motility Revealed by Tether-Scanning of the Motor Core

Yamagishi, M., SUMIYOSHI, R., & Yajima, J. (2026) · Seibutsu Butsuri · doi:10.2142/biophys.66.149

Shaping animal body plans in development and evolution by modulation of Hox expression patterns

(1998) · doi:10.1002/(sici)1521-1878(199802)20:2

An N-terminal truncation of the ncd motor protein supports diffusional movement of microtubules in motility assays

Chandra, R., Endow, S. A., & Salmon, E. D. (1993) · Journal of Cell Science · doi:10.1242/jcs.104.3.899 · Cited by 37

The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motor

McDonald, H. B., Stewart, R. J., & Goldstein, L. S. B. (1990) · Cell · doi:10.1016/0092-8674(90)90412-8 · Cited by 342

Structural and functional domains of the Drosophila ncd microtubule motor protein.

Chandra, R., Salmon, E. D., Erickson, H., Lockhart, A., & Endow, S. A. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)52971-9 · Cited by 157

Motor domains of kinesin and ncd interact with microtubule protofilaments with the same binding geometry

Hoenger, A., & Milligan, R. A. (1997) · Journal of Molecular Biology · doi:10.1006/jmbi.1996.0757 · Cited by 60

Reversal in the direction of movement of a molecular motor

Henningsen, U., & Schliwa, M. (1997) · Nature · doi:10.1038/38022 · Cited by 204