Association of integrase, matrix, and reverse transcriptase antigens of human immunodeficiency virus type 1 with viral nucleic acids following acute infection.
Open accessAbstract
We have examined components of the preintegration complex of human immunodeficiency virus type 1 (HIV-1) and have analyzed features which govern the association of these components. HIV-1 nucleoprotein complexes, isolated from nuclear and cytoplasmic extracts of CD4+ cells after acute virus infection, contained viral RNA and DNA in association with viral matrix (MA), integrase (IN), and reverse transcriptase (RT) antigens but not capsid (CA) antigens and possessed integration activity in vitro. Association of IN but not RT or MA antigens with viral DNA was detergent-stable. Analysis of viral DNA synthesis and nuclear import of viral nucleoprotein complexes in the presence of a reversible RT inhibitor demonstrated that reverse transcription of viral RNA could be completed entirely in the host cell nucleus. Our studies demonstrate structural and functional features of the nucleoprotein (preintegration) complex of HIV-1 which are pertinent to the understanding of early events in the lentiviral life cycle.
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The reverse transcription complex is actively transported to the chromosomal DNA, where integration may occur.
“This RTC is actively transported to the chromosomal DNA, where integration may occur [6–9].”
From Clinical use of lentiviral vectors (2018) · cites this paper as [6]
Cites
Active nuclear import of human immunodeficiency virus type 1 preintegration complexes.
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HIV-1 Integration in the Human Genome Favors Active Genes and Local Hotspots
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Clinical use of lentiviral vectors
Milone, M. C., & O’Doherty, U. (2018) · Leukemia · doi:10.1038/s41375-018-0106-0 · Cited by 930 · see citing works
“One plus-strand is initiated at U3, and one is initiated at the central PPT (cPPT) [6].”
BackgroundHIV-1 Gag proteins: diverse functions in the virus life cycle
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HIV-1 uncoats in the nucleus near sites of integration
Burdick, R. C., Li, C., Munshi, M., Rawson, J. M. O., Nagashima, K., Hu, W.-S., & Pathak, V. K. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1920631117 · Cited by 313 · see citing works
Capsid Is a Dominant Determinant of Retrovirus Infectivity in Nondividing Cells
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Complementary assays reveal a relationship between HIV-1 uncoating and reverse transcription
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Structural and sequencing analysis of local target DNA recognition by MLV integrase
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- Authors
- Bukrinsky, M. I., Sharova, N., McDonald, T. L., Pushkarskaya, T., Tarpley, W. G., & Stevenson, M.
- Affiliations
- University of Nebraska Medical Center (US)
- École Polytechnique Fédérale de Lausanne (CH)
- Pfizer (United States) (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1993-07
- Volume
- 90
- Issue
- 13
- Pages
- 6125-6129
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC46880
- OpenAlex
- W2076634735
- Cited by
- 451
- References
- 1
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 5 · 2022: 7 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 10 · 2017: 2 · 2016: 5 · 2015: 13 · 2014: 11 · 2013: 18 · 2012: 20
- FWCI
- 7.52
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ai030386-02)
- National Institute of Allergy and Infectious Diseases (AI-32890, AI-30386, R01AI032890)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Keywords
- HIV-1, preintegration complex, integrase, matrix protein, reverse transcriptase, viral DNA replication, nuclear import, reverse transcription, acute infection, CD4+ T cells, viral integration
- MeSH terms
- Base Sequence, Cell Nucleus, DNA Nucleotidyltransferases, DNA, Viral, Humans, Molecular Sequence Data, RNA-Directed DNA Polymerase, RNA, Viral, Transcription, Genetic, Viral Matrix Proteins, Virus Replication, CD4-Positive T-Lymphocytes, HIV-1, Virus Integration, Integrases, HIV Reverse Transcriptase
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