The DOI matches its Crossref record.
Expression cloning of a high-affinity melatonin receptor from Xenopus dermal melanophores.
Open accessAbstract
Using an expression cloning strategy, a high-affinity melatonin receptor cDNA has been isolated from Xenopus laevis dermal melanophores. Transient expression of the cDNA in COS-7 cells resulted in high-affinity 2-[125I]-iodomelatonin binding (Kd = 6.3 +/- 0.3 x 10(-11) M). In addition, six ligands exhibited a rank order of inhibition of specific 2-[125I]iodomelatonin binding that was identical to that reported for endogenous high-affinity receptors. Functional studies of CHO cells stably expressing the receptor cDNA showed that melatonin acting through the cloned receptor inhibited forskolin-stimulated cAMP accumulation in a dose-dependent manner. Northern blot analysis showed that melatonin receptor transcripts are moderately expressed in Xenopus dermal melanophores. The cDNA encodes a protein of 420 amino acids, which contains seven hydrophobic segments. Structural analysis revealed that the receptor protein is a newly discovered member of the guanine nucleotide binding protein-coupled receptor family.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Brzezinski, A. (1997) · New England Journal of Medicine · doi:10.1056/nejm199701163360306 · Cited by 1,549
Reppert, S. M., Weaver, D. R., & Ebisawa, T. (1994) · Neuron · doi:10.1016/0896-6273(94)90055-8 · Cited by 1,096
Self-citationMolecular pharmacology regulation and function of mammalian melatonin receptors
Dubocovich, M. L. (2003) · Frontiers in Bioscience · doi:10.2741/1089 · Cited by 451
Rapid color change in fish and amphibians – function, regulation, and emerging applications
Nilsson Sköld, H., Aspengren, S., & Wallin, M. (2013) · Pigment Cell & Melanoma Research · doi:10.1111/pcmr.12040 · Cited by 329
Melatonin receptors, heterodimerization, signal transduction and binding sites: what's new?
Jockers, R., Maurice, P., Boutin, J. A., & Delagrange, P. (2008) · British Journal of Pharmacology · doi:10.1038/bjp.2008.184 · Cited by 293
Metadata
- Authors
- Ebisawa, T., Karne, S., Lerner, M. R., & Reppert, S. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1994-06-21
- Volume
- 91
- Issue
- 13
- Pages
- 6133-6137
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2002226670
- Cited by
- 432
- References
- 0
- FWCI
- 17.66
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Expression cloning of a high-affinity melatonin receptor from Xenopus dermal melanophores. https://app.cochat.ai/papers/10.1073/pnas.91.13.6133
- The paper
- Ebisawa, T., Karne, S., Lerner, M. R., & Reppert, S. M. (1994). Expression cloning of a high-affinity melatonin receptor from Xenopus dermal melanophores. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.91.13.6133
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
