The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Protein-tyrosine-phosphatase SHPTP2 couples platelet-derived growth factor receptor beta to Ras.
Open accessAbstract
Protein-tyrosine-phosphatase SHPTP2 (Syp/PTP-1D/PTP2C) is the homologue of the Drosophila corkscrew (csw) gene product, which transmits positive signals from receptor tyrosine kinases. Likewise, SHPTP2 has been implicated in positive signaling from platelet-derived growth factor receptor beta (PDGFR). Upon PDGF stimulation, SHPTP2 binds to the PDGFR and becomes tyrosine-phosphorylated. We have identified tyrosine-542 (pY542TNI) as the major in vivo site of SHPTP2 tyrosine phosphorylation. The pY542TNI sequence conforms to the consensus binding site for the SH2 domain of Grb2, which, by association with Sos1, couples some growth factor receptors to Ras. Following PDGF stimulation, Grb2 binds tyrosine-phosphorylated SHPTP2. Moreover, a mutant PDGFR lacking its SHPTP2 binding site displays markedly reduced Grb2 binding. These data indicate that phosphorylation of SHPTP2 couples Grb2 to PDGFR in vivo, providing a mechanism for Ras activation by PDGFR and for positive signaling via SHPTP2 and Csw.
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“SHP2 is required for full activation of SOS1/2, whereas SOS1 inhibitors are inactive against SOS2.”
From The current state of the art and future trends in RAS-targeted cancer therapies (2022) · cites this paper as [142]
In the citing paper
“which is recruited to many receptor tyrosine kinases upon activation and is generally a positive regulator of Ras/MAPK signaling (Perkins et al., 1996; Bennett et al., 1994; Tang et al., 1995; Maroun et al., 2000; Oishi et al., 2006).”
From The Phosphatase SHP2 Regulates the Spacing Effect for Long-Term Memory Induction (2009) · cites this paper as Bennett et al., 1994
Citations
Cites
Signal transduction by receptors with tyrosine kinase activity
Ullrich, A., & Schlessinger, J. (1990) · Cell · doi:10.1016/0092-8674(90)90801-k · Cited by 5,435
Gene Splicing by Overlap Extension: Tailor-Made Genes Using the Polymerase Chain Reaction
Horton, R. M., Cai, Z., Ho, S. N., & Pease, L. R. (2013) · BioTechniques · doi:10.2144/000114017 · Cited by 1,292
Egan, S. E., Giddings, B. W., Brooks, M. W., Buday, L., Sizeland, A. M., & Weinberg, R. A. (1993) · Nature · doi:10.1038/363045a0 · Cited by 1,200
[42] Detection and quantification of phosphotyrosine in proteins
Cooper, J. A., Sefton, B. M., & Hunter, T. R. (1983) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(83)99075-4 · Cited by 1,109
Buday, L., & Downward, J. (1993) · Cell · doi:10.1016/0092-8674(93)90146-h · Cited by 1,064
The SH2 and SH3 domains of mammalian Grb2 couple the EGF receptor to the Ras activator mSos1
Rozakis-Adcock, M., Fernley, R. T., Wade, J. D., Pawson, T. J., & Bowtell, D. D. L. (1993) · Nature · doi:10.1038/363083a0 · Cited by 1,009
Phosphatidylinositol 3′‐kinase is activated by association with IRS‐1 during insulin stimulation.
Backer, J., Myers, M. G., Shoelson, S. E., Chin, D. J., Sun, X., Miralpeix, M., Hu, P. J., Margolis, B. L., Skolnik, E. Y., & Schlessinger, J. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05426.x · Cited by 1,000
Rozakis-Adcock, M., McGlade, J. C., Mbamalu, G., Pelicci, G., Daly, R. J., Li, W., Batzer, A., Thomas, S. Μ., Brugge, J. S., Pelicci, P. G., et al. (1992) · Nature · doi:10.1038/360689a0 · Cited by 973
SH2 and SH3 domains: From structure to function
Pawson, T. J., & Gish, G. D. (1992) · Cell · doi:10.1016/0092-8674(92)90504-6 · Cited by 940
Li, N., Batzer, A., Daly, R. J., Yajnik, V., Skolnik, E. Y., Chardin, P., Bar‐Sagi, D., Margolis, B. L., & Schlessinger, J. (1993) · Nature · doi:10.1038/363085a0 · Cited by 938
Skolnik, E. Y., Lee, C., Batzer, A., Vicentini, L. M., Zhou, M., Daly, R. J., Myers, M. G., Backer, J., Ullrich, A., & White, M. F. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb05842.x · Cited by 686
SH2-Containing Phosphotyrosine Phosphatase as a Target of Protein-Tyrosine Kinases
Feng, G., Hui, C., & Pawson, T. J. (1993) · Science · doi:10.1126/science.8096088 · Cited by 663
Valius, M., & Kazlauskas, A. (1993) · Cell · doi:10.1016/0092-8674(93)90232-f · Cited by 640
Association between the PDGF receptor and members of the src family of tyrosine kinases
Kypta, R. M., Goldberg, Y., Ulug, E. T., & Courtneidge, S. A. (1990) · Cell · doi:10.1016/0092-8674(90)90013-5 · Cited by 635
Increase of the Catalytic Activity of Phospholipase C-γ1 by Tyrosine Phosphorylation
Nishibe, S., Wahl, M. I., Hernández‐Sotomayor, S. M. T., Tonks, N. K., Rhee, S. G., & Carpenter, G. F. (1990) · Science · doi:10.1126/science.1700866 · Cited by 635
Kmiecik, T. E., & Shalloway, D. I. (1987) · Cell · doi:10.1016/0092-8674(87)90756-2 · Cited by 623
ras GTPase activating protein: Signal transmitter and signal terminator
McCormick, F. (1989) · Cell · doi:10.1016/0092-8674(89)90976-8 · Cited by 607
Grb2 mediates the EGF-dependent activation of guanine nucleotide exchange on Ras
Gale, N. W., Kaplan, S., Lowenstein, E. J., Schlessinger, J., & Bar‐Sagi, D. (1993) · Nature · doi:10.1038/363088a0 · Cited by 563
Activation of a Phosphotyrosine Phosphatase by Tyrosine Phosphorylation
Vogel, W. F., Lammers, R., Huang, J., & Ullrich, A. (1993) · Science · doi:10.1126/science.7681217 · Cited by 549
Olivier, J. P. (1993) · Cell · doi:10.1016/0092-8674(93)90170-u · Cited by 537
Tyrosine phosphorylation regulates the biochemical and biological properties of pp60c-src
Piwnica-Worms, H. M., Saunders, K. B., Roberts, T. M., Smith, A. E., & Cheng, S. H. (1987) · Cell · doi:10.1016/0092-8674(87)90757-4 · Cited by 520
A new function for a phosphotyrosine phosphatase: linking GRB2-Sos to a receptor tyrosine kinase
Li, W., Nishimura, R., Kashishian, A., Batzer, A., Kim, W. J. H., Cooper, J. A., & Schlessinger, J. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.1.509-517.1994 · Cited by 481
Pleiotropic Insulin Signals are Engaged by Multisite Phosphorylation of IRS-1
Sun, X., Crimmins, D. L., Myers, M. G., Miralpeix, M., & White, M. F. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.12.7418 · Cited by 465
Simon, M. (1993) · Cell · doi:10.1016/0092-8674(93)90169-q · Cited by 441
Perkins, L. A., Larsen, I., & Perrimon, N. (1992) · Cell · doi:10.1016/0092-8674(92)90098-w · Cited by 390
Cell transformation by pp60c-src mutated in the carboxy-terminal regulatory domain
Cartwright, C. A., Eckhart, W., Simon, S., & Kaplan, P. L. (1987) · Cell · doi:10.1016/0092-8674(87)90758-6 · Cited by 390
Carpenter, C. L., Auger, K. R., Chanudhuri, M., Yoakim, M., Schaffhausen, B. S., Shoelson, S. E., & Cantley, L. C. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)98375-4 · Cited by 384
The insulin receptor substrate 1 associates with the SH2-containing phosphotyrosine phosphatase Syp
Kuhné, M. R., Pawson, T. J., Lienhard, G. E., & Feng, G. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50220-4 · Cited by 380
Freeman, R. M., Plutzky, J., & Neel, B. G. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.23.11239 · Cited by 362
Self-citationLechleider, R. J., Sugimoto, S., Bennett, A. M., Kashishian, A., Cooper, J. A., Shoelson, S. E., Walsh, C. T., & Neel, B. G. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)80562-6 · Cited by 319
Self-citationKazlauskas, A., Kashishian, A., Cooper, J. A., & Valius, M. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.6.2534 · Cited by 228
A widely expressed human protein-tyrosine phosphatase containing src homology 2 domains.
Ahmad, S., Banville, D. L., Zhao, Z., Fischer, E. H., & Shen, S. H. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.6.2197 · Cited by 212
Valius, M., Bazenet, C. E., & Kazlauskas, A. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.1.133 · Cited by 209
Ralston, R., & Bishop, J. M. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.23.7845 · Cited by 203
Lechleider, R. J., Freeman, R. M., & Neel, B. G. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)38668-5 · Cited by 191
Self-citationShoelson, S. E., Sivaraja, M., Williams, K. P., Hu, P. J., Schlessinger, J., & Weiss, M. A. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb05714.x · Cited by 169
Lck-Dependent Tyrosyl Phosphorylation of the Phosphotyrosine Phosphatase SH-PTP1 in Murine T Cells
Lorenz, U. M., Ravichandran, K. S., Pei, D., Walsh, C. T., Burakoff, S. J., & Neel, B. G. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.3.1824 · Cited by 153
Self-citationLu, X., Chou, T.-B., Williams, N. G., Roberts, T. M., & Perrimon, N. (1993) · Genes & Development · doi:10.1101/gad.7.4.621 · Cited by 108
Sugimoto, S., Lechleider, R. J., Shoelson, S. E., Neel, B. G., & Walsh, C. T. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)41593-1 · Cited by 86
Self-citationKashishian, A., & Cooper, J. A. (1993) · Molecular Biology of the Cell · doi:10.1091/mbc.4.1.49 · Cited by 73
Frangioni, J. V., & Neel, B. G. (1993) · Journal of Cell Science · doi:10.1242/jcs.105.2.481 · Cited by 54
Self-citationKazlauskas, A., Kashishian, A., Cooper, J. A., & Valius, M. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.6.2534-2544.1992 · Cited by 36
Lck-Dependent Tyrosyl Phosphorylation of the Phosphotyrosine Phosphatase SH-PTP1 in Murine T Cells
Lorenz, U. M., Ravichran, K. S., Pei, D., et al. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.3.1824-1834.1994 · Cited by 34
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Metadata
- Authors
- Bennett, A. M., Tang, T. L., Sugimoto, S., Walsh, C. T., & Neel, B. G.
- Affiliations
- Beth Israel Deaconess Medical Center (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1994-07-19
- Volume
- 91
- Issue
- 15
- Pages
- 7335-7339
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC44394
- OpenAlex
- W2006147172
- Cited by
- 397
- References
- 43
- Citations by year
- 2026: 8 · 2025: 4 · 2024: 4 · 2023: 9 · 2022: 11 · 2021: 13 · 2020: 7 · 2019: 6 · 2018: 10 · 2017: 5 · 2016: 10 · 2015: 4 · 2014: 5 · 2013: 10 · 2012: 7
- FWCI
- 12.78
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Cancer Society
- National Institutes of Health (GM20011, CA49152)
- National Cancer Institute (CA-49152)
- National Institute of General Medical Sciences (GM 20011)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Keywords
- PDGFRβ, Ras activation, Grb2, SOS1, receptor tyrosine kinase signaling, tyrosine phosphorylation, SH2 domain binding
- MeSH terms
- Animals, Base Sequence, Binding Sites, Cell Line, DNA, Humans, Mice, Inbred BALB C, Molecular Sequence Data, Phosphorylation, Genes, ras, Signal Transduction, 3T3 Cells, Protein Tyrosine Phosphatases, Receptors, Platelet-Derived Growth Factor, Intracellular Signaling Peptides and Proteins, Mice, Protein Tyrosine Phosphatase, Non-Receptor Type 6, Protein Tyrosine Phosphatase, Non-Receptor Type 11, SH2 Domain-Containing Protein Tyrosine Phosphatases
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