The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Atherosclerosis and sterol 27-hydroxylase: evidence for a role of this enzyme in elimination of cholesterol from human macrophages.
Open accessAbstract
27-Hydroxycholesterol was found in surprisingly high amounts in atherosclerotic human femoral arteries. When human macrophages were cultured in a medium containing serum, there was a significant transfer of 27-hydroxy-cholesterol and 3 beta-hydroxy-5-cholestenoic acid from the cells into the medium. Sterol 27-hydroxylase (EC 1.14.13.15) is likely to be responsible for formation of the two products as shown by use of immunoblotting, a specific inhibitor, and the 18O-labeling technique. Sterol 27-hydroxylase has the unusual ability to hydroxylate the same methyl group three times to give a carboxylic acid; thus, 3 beta-hydroxy-5-cholestenoic acid is likely to be a direct product of the enzyme. The production of these steroids increased after addition of cholesterol to the culture medium. By using deuterium-labeled cholesterol, it was ascertained that most of the oxidized products were formed from exogenous cholesterol taken up by the cells. 27-Hydroxycholesterol and 3 beta-hydroxy-5-cholestenoic acid are present in the circulation and are efficiently converted into bile acids in human liver. It is suggested that conversion of cholesterol into 27-hydroxycholesterol and 3 beta-hydroxy-5-cholestenoic acid represents a general defence mechanism for macrophages and possibly also other peripheral cells exposed to cholesterol. Absence of this defence mechanism may contribute to the premature atherosclerosis known to occur in patients with sterol 27-hydroxylase deficiency (cerebrotendinous xanthomatosis).
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Cites
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Self-citationMechanism of degradation of the steroid side chain in the formation of bile acids.
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Cited by
Lütjohann, D., Breuer, O., Ahlborg, G., Nennesmo, I., Sidén, A., Diczfalusy, U., & Björkhem, I. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.18.9799 · Cited by 682
Self-citationcDNA cloning of cholesterol 24-hydroxylase, a mediator of cholesterol homeostasis in the brain
Lund, E. G., Guileyardo, J. M., & Russell, D. W. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.13.7238 · Cited by 668
Self-citationRegulation of bile acid synthesis
Chiang, J. Y. L. (1998) · Frontiers in Bioscience · doi:10.2741/a273 · Cited by 393
Lacapère, J.-J., & Papadopoulos, V. (2003) · Steroids · doi:10.1016/s0039-128x(03)00101-6 · Cited by 342
Lund, E. G., Kerr, T. A., Sakai, J., Li, W.-P., & Russell, D. W. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.51.34316 · Cited by 335
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Metadata
- Authors
- Björkhem, I., Andersson, O., Diczfalusy, U., Sevastik, B., Xiu, R. J., Duan, C., & Lund, E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1994-08-30
- Volume
- 91
- Issue
- 18
- Pages
- 8592-8596
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2086831301
- Cited by
- 320
- References
- 25
- FWCI
- 18.11
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Atherosclerosis and sterol 27-hydroxylase: evidence for a role of this enzyme in elimination of cholesterol from human macrophages. https://app.cochat.ai/papers/10.1073/pnas.91.18.8592
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- Björkhem, I., Andersson, O., Diczfalusy, U., Sevastik, B., Xiu, R. J., Duan, C., & Lund, E. (1994). Atherosclerosis and sterol 27-hydroxylase: evidence for a role of this enzyme in elimination of cholesterol from human macrophages. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.91.18.8592
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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