The DOI matches its Crossref record.
Construction and characterization of a normalized cDNA library.
Open accessAbstract
We have developed a simple procedure based on reassociation kinetics that can reduce effectively the high variation in abundance among the clones of a cDNA library that represent individual mRNA species. For this normalization, we used as a model system a library of human infant brain cDNAs that were cloned directionally into a phagemid vector and, thus, could be easily converted into single-stranded circles. After controlled primer extension to synthesize a short complementary strand on each circular template, melting and reannealing of the partial duplexes at relatively low C0t, and hydroxyapatite column chromatography, unreassociated circles were recovered from the flow through fraction and electroporated into bacteria, to propagate a normalized library without a requirement for subcloning steps. An evaluation of the extent of normalization has indicated that, from an extreme range of abundance of 4 orders of magnitude in the original library, the frequency of occurrence of any clone examined in the normalized library was brought within the narrow range of only 1 order of magnitude.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Identification of the Tuberous Sclerosis Gene TSC1 on Chromosome 9q34
Slegtenhorst, M. V., Hoogt, R. D., Hermans, C., Nellist, M., Janssen, B., Verhoef, S., Lindhout, D., Ouweland, A. V. D., Halley, D., Young, J., et al. (1997) · Science · doi:10.1126/science.277.5327.805 · Cited by 1,648
Isolation of a novel gene underlying batten disease, CLN3
Lerner, T. J., Boustany, R.-M. N., Anderson, J. W., D'Arigo, K. L., Schlumpf, K., Buckler, A. J., Gusella, J. F., & Haines, J. L. (1995) · Cell · doi:10.1016/0092-8674(95)90274-0 · Cited by 601
Normalization and subtraction: two approaches to facilitate gene discovery.
Bonaldo, M. F., Lennon, G., & Soares, M. B. (1996) · Genome Research · doi:10.1101/gr.6.9.791 · Cited by 522
Self-citationParchman, T. L., Geist, K. S., Grahnen, J. A., Benkman, C. W., & Buerkle, C. A. (2010) · BMC Genomics · doi:10.1186/1471-2164-11-180 · Cited by 446
Insights into corn genes derived from large-scale cDNA sequencing
Alexandrov, N. N., Brover, V. V., Freidin, S., Troukhan, M. E., Tatarinova, T. V., Zhang, H., Swaller, T. J., Lu, Y.-P., Bouck, J., Flavell, R. B., et al. (2009) · Plant Molecular Biology · doi:10.1007/s11103-008-9415-4 · Cited by 248
Metadata
- Authors
- Soares, M. B., Bonaldo, M. F., Jelene, P., Su, L., Lawton, L., & Efstratiadis, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1994-09-27
- Volume
- 91
- Issue
- 20
- Pages
- 9228-9232
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2074242397
- Cited by
- 410
- References
- 0
- FWCI
- 7.97
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Construction and characterization of a normalized cDNA library. https://app.cochat.ai/papers/10.1073/pnas.91.20.9228
- The paper
- Soares, M. B., Bonaldo, M. F., Jelene, P., Su, L., Lawton, L., & Efstratiadis, A. (1994). Construction and characterization of a normalized cDNA library. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.91.20.9228
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
