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In vivo transfer and expression of a human epidermal growth factor gene accelerates wound repair.
Open accessAbstract
This report details the transfer of a human epidermal growth factor (hEGF) expression plasmid to porcine partial-thickness wound keratinocytes by particle-mediated DNA transfer (Accell). After gene transfer an external sealed fluid-filled wound chamber was used to protect the wound, provide containment of the exogenous DNA and expressed peptide, and permit sampling of the wound fluid. Analysis of wound fluid for hEGF and total protein, an indicator of reformation of the epithelial barrier, showed that wounds bombarded with the hEGF plasmid exhibited a 190-fold increase in EGF concentration and healed 20% (2.1 days) earlier than the controls. EGF concentrations in wound fluid persisted over the entire 10-day monitored period, decreasing from 200 pg/ml to 25 pg/ml over the first 5 days. Polymerase chain reaction results showed that plasmid DNA was present in the wound for at least 30 days. These findings demonstrate the possible utility of in vivo gene transfer to enhance epidermal repair.
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Cited by
Broughton, G., Janis, J. E., & Attinger, C. E. (2006) · Plastic and Reconstructive Surgery · doi:10.1097/01.prs.0000222562.60260.f9 · Cited by 917
Wound healing: Biologic features and approaches to maximize healing trajectories
Robson, M. C., Steed, D. L., & Franz, M. G. (2001) · Current Problems in Surgery · doi:10.1067/msg.2001.111167 · Cited by 503
HoxD3 Accelerates Wound Healing in Diabetic Mice
Hansen, S. L., Myers, C. A., Charboneau, A., Young, D. M., & Boudreau, N. (2003) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)63597-3 · Cited by 82
Cytokine gene expression in a murine wound healing model
Bryan, D., Walker, K. B., Ferguson, M., & Thorpe, R. (2005) · Cytokine · doi:10.1016/j.cyto.2005.06.015 · Cited by 80
Metadata
- Authors
- Andree, C., Swain, W. F., Page, C. P., Macklin, M. D., Slama, J., Hatzis, D., & Eriksson, E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1994-12-06
- Volume
- 91
- Issue
- 25
- Pages
- 12188-12192
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2001297140
- Cited by
- 211
- References
- 0
- FWCI
- 11.27
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: In vivo transfer and expression of a human epidermal growth factor gene accelerates wound repair. https://app.cochat.ai/papers/10.1073/pnas.91.25.12188
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- Andree, C., Swain, W. F., Page, C. P., Macklin, M. D., Slama, J., Hatzis, D., & Eriksson, E. (1994). In vivo transfer and expression of a human epidermal growth factor gene accelerates wound repair. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.91.25.12188
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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