The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
2-Methoxyestradiol, an endogenous mammalian metabolite, inhibits tubulin polymerization by interacting at the colchicine site.
Open accessAbstract
A metabolite of estradiol, 2-methoxyestradiol (2ME), inhibits angiogenesis in the chicken embryo chorioallantoic membrane assay. Since 2ME causes mitotic perturbations, we examined its interactions with tubulin. In our standard 1.0 M glutamate system (plus 1.0 mM MgCl2 at 37 degrees C), superstoichiometric concentrations (relative to tubulin) of 2ME inhibited the nucleation and propagation phases of tubulin assembly but did not affect the reaction extent. Although polymer formed in the presence of 2ME was more cold-stable than control polymer, morphology was little changed. Under suboptimal reaction conditions (0.8 M glutamate/no MgCl2 at 26 degrees C), substoichiometric 2ME totally inhibited polymerization. No other estrogenic compound was as effective as 2ME as an inhibitor of polymerization or of the binding of colchicine to tubulin. Inhibition of colchicine binding was competitive (Ki, 22 microM). Thus, a mammalian metabolite of estradiol binds to the colchicine site of tubulin and, depending on reaction conditions, either inhibits assembly or seems to be incorporated into a polymer with altered stability properties.
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Fotsis, T., Zhang, Y., Pepper, M. S., Adlercreutz, H., Montesano, R., Nawroth, P. P., & Schweigerer, L. (1994) · Nature · doi:10.1038/368237a0 · Cited by 736
A New Class of Steroids Inhibits Angiogenesis in the Presence of Heparin or a Heparin Fragment
Crum, R., Szabo, S., & Folkman, J. (1985) · Science · doi:10.1126/science.2416056 · Cited by 488
Self-citationLin, C. M., Singh, S. B., Chu, P. S., Dempcy, R. O., Schmidt, J. M., Pettit, G. R., & Hamel, E. (1988) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)09364-2 · Cited by 278
Self-citationHamel, E., & Lin, C. M. (1984) · Biochemistry · doi:10.1021/bi00313a026 · Cited by 226
Self-citationColchicine inhibition of microtubule assembly via copolymer formation.
Sternlicht, H., & Ringel, I. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)86741-8 · Cited by 146
Seegers, J. C., Aveling, M.-L., van Aswegen, C. H., et al. (1989) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(89)90455-x · Cited by 145
Fltzgerald, T. J. (1976) · Biochemical Pharmacology · doi:10.1016/0006-2952(76)90108-8 · Cited by 145
Berg, D., Sonsalla, R., & Kuss, E. (1983) · European Journal of Endocrinology · doi:10.1530/acta.0.1030282 · Cited by 114
Merriam, G. R., MacLusky, N. J., Picard, M. K., & Naftolin, F. N. (1980) · Steroids · doi:10.1016/0039-128x(80)90062-8 · Cited by 111
Lacey, E., & Watson, T. R. (1985) · Biochemical Pharmacology · doi:10.1016/0006-2952(85)90611-2 · Cited by 84
Cited by
Männistö, P. T., & Kaakkola, S. (1999) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01423-6 · Cited by 1,068
2ME2 inhibits tumor growth and angiogenesis by disrupting microtubules and dysregulating HIF
Mabjeesh, N. J., Escuin, D., LaVallee, T. M., Pribluda, V. S., Swartz, G. M., Johnson, M. S., Willard, M. T., Zhong, H., Simons, J. W., & Giannakakou, P. (2003) · Cancer Cell · doi:10.1016/s1535-6108(03)00077-1 · Cited by 730
Molecular mechanisms of novel therapeutic approaches for multiple myeloma
Hideshima, T., & Anderson, K. C. (2002) · Nature Reviews Cancer · doi:10.1038/nrc952 · Cited by 425
Deficiency in catechol-O-methyltransferase and 2-methoxyoestradiol is associated with pre-eclampsia
Kanasaki, K., Palmsten, K., Sugimoto, H., Ahmad, S., Hamano, Y., Xie, L., Parry, S., Augustin, H. G., Gattone, V. H., Folkman, J., et al. (2008) · Nature · doi:10.1038/nature06951 · Cited by 381
Hamel, E. (2003) · Cell Biochemistry and Biophysics · doi:10.1385/cbb:38:1:1 · Cited by 272
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Metadata
- Authors
- D'Amato, R. J., Lin, C. M., Flynn, E., Folkman, J., & Hamel, E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1994-04-26
- Volume
- 91
- Issue
- 9
- Pages
- 3964-3968
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2034426069
- Cited by
- 406
- References
- 17
- FWCI
- 2.01
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: 2-Methoxyestradiol, an endogenous mammalian metabolite, inhibits tubulin polymerization by interacting at the colchicine site. https://app.cochat.ai/papers/10.1073/pnas.91.9.3964
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- D'Amato, R. J., Lin, C. M., Flynn, E., Folkman, J., & Hamel, E. (1994). 2-Methoxyestradiol, an endogenous mammalian metabolite, inhibits tubulin polymerization by interacting at the colchicine site. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.91.9.3964
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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