The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Cloning and functional expression of a rat kidney extracellular calcium/polyvalent cation-sensing receptor.
Open accessAbstract
The maintenance of a stable extracellular concentration of ionized calcium depends on the integrated function of a number of specialized cells (e.g., parathyroid and certain kidney epithelial cells). We recently identified another G protein-coupled receptor (BoPCaRI) from bovine parathyroid that responds to changes in extracellular Ca2+ within the millimolar range and provides a key mechanism for regulating the secretion of parathyroid hormone. Using an homology-based strategy, we now report the isolation of a cDNA encoding an extracellular Ca2+/polyvalent cation-sensing receptor (RaKCaR) from rat kidney. The predicted RaKCaR protein shares 92% identity with BoPCaR1 receptor and features a seven membrane-spanning domain, characteristic of the G protein-coupled receptors, which is preceded by a large hydrophilic extracellular NH2 terminus believed to be involved in cation binding. RaKCaR cRNA-injected Xenopus oocytes responded to extracellular Ca2+, Mg2+, Gd3+, and neomycin with characteristic activation of inositol phospholipid-dependent, intracellular Ca(2+)-induced Cl- currents. In rat kidney, Northern analysis revealed RaKCaR transcripts of 4 and 7 kb, and in situ hybridization showed localization primarily in outer medulla and cortical medullary rays. Our results provide important insights into the molecular structure of an extracellular Ca2+/polyvalent cation-sensing receptor in rat kidney and provide another basis on which to understand the role of extracellular divalent cations in regulating kidney function in mineral metabolism.
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Metadata
- Authors
- Riccardi, D., Park, J., Lee, W. S., Gamba, G., Brown, E. M., & Hebert, S. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1995-01-03
- Volume
- 92
- Issue
- 1
- Pages
- 131-135
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2077973233
- Cited by
- 466
- References
- 23
- FWCI
- 36.55
- Type
- journal article
- Language
- en
- Open access
- green
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- Riccardi, D., Park, J., Lee, W. S., Gamba, G., Brown, E. M., & Hebert, S. C. (1995). Cloning and functional expression of a rat kidney extracellular calcium/polyvalent cation-sensing receptor. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.92.1.131
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