The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Building zinc fingers by selection: toward a therapeutic application.
Open accessAbstract
A phage display approach was utilized to modify the specificity of each of the three fingers of the murine transcription factor Zif268. Selections were performed by using the consensus binding sequence of the natural protein and a conserved sequence in the genome of the type 1 human immunodeficiency virus. By using an extensive randomization strategy, the entire 3-bp specificity of a finger has been changed. Rapid analysis of selected zinc fingers was facilitated by the development of an immunoscreening assay for DNA binding and specificity. To investigate the mechanism of binding and specificity, the binding kinetics of Zif268 and 10 selected variants were determined in real time with an assay based on surface plasmon resonance. Differential mechanisms for sequence-specific recognition were observed. No evidence in support of a single general coding relationship between zinc finger and target DNA sequence was observed. The prospects for the development of this class of proteins in human therapy are considered.
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Cites
Crystal Structure of a Five-Finger GLI-DNA Complex: New Perspectives on Zinc Fingers
Pavletich, N. P., & Pabo, C. O. (1993) · Science · doi:10.1126/science.8378770 · Cited by 669
Cited by
Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain.
Kim, Y. G., Cha, J., & Chandrasegaran, S. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.3.1156 · Cited by 1,889
DNA Recognition by Cys2His2 Zinc Finger Proteins
Wolfe, S. A., Nekludova, L., & Pabo, C. O. (2000) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.29.1.183 · Cited by 1,053
Genome-editing Technologies for Gene and Cell Therapy
Maeder, M. L., & Gersbach, C. A. (2016) · Molecular Therapy · doi:10.1038/mt.2016.10 · Cited by 719
Protein Design: Toward Functional Metalloenzymes
Yu, F., Cangelosi, V. M., Zastrow, M. L., Tegoni, M., Plegaria, J. S., Tebo, A. G., Mocny, C. S., Ruckthong, L., Qayyum, H., & Pecoraro, V. L. (2014) · Chemical Reviews · doi:10.1021/cr400458x · Cited by 441
Elrod-Erickson, M., Rould, M. A., Nekludova, L., & Pabo, C. O. (1996) · Structure · doi:10.1016/s0969-2126(96)00125-6 · Cited by 421
Metadata
- Authors
- Wu, H., Yang, W. P., & Barbas, C. F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1995-01-17
- Volume
- 92
- Issue
- 2
- Pages
- 344-348
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2031675476
- Cited by
- 195
- References
- 1
- FWCI
- 4.62
- Type
- journal article
- Language
- en
- Open access
- green
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- Wu, H., Yang, W. P., & Barbas, C. F. (1995). Building zinc fingers by selection: toward a therapeutic application. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.92.2.344
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
