The DOI matches its Crossref record.
Magnesium-protoporphyrin chelatase of Rhodobacter sphaeroides: reconstitution of activity by combining the products of the bchH, -I, and -D genes expressed in Escherichia coli.
Open accessAbstract
Magnesium-protoporphyrin chelatase lies at the branch point of the heme and (bacterio)chlorophyll biosynthetic pathways. In this work, the photosynthetic bacterium Rhodobacter sphaeroides has been used as a model system for the study of this reaction. The bchH and the bchI and -D genes from R. sphaeroides were expressed in Escherichia coli. When cell-free extracts from strains expressing BchH, BchI, and BchD were combined, the mixture was able to catalyze the insertion of Mg into protoporphyrin IX in an ATP-dependent manner. This was possible only when all three genes were expressed. The bchH, -I, and -D gene products are therefore assigned to the Mg chelatase step in bacteriochlorophyll biosynthesis. The mechanism of the Mg chelation reaction and the implications for chlorophyll biosynthesis in plants are discussed.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Von Wettstein, D., Gough, S., & Kannangara, C. G. (1995) · The Plant Cell · doi:10.1105/tpc.7.7.1039 · Cited by 339
Self-citationTetrapyrrole Metabolism in Arabidopsis thaliana
Tanaka, R., Kobayashi, K., & Masuda, T. (2011) · The Arabidopsis Book · doi:10.1199/tab.0145 · Cited by 281
Tsuzuki, T., Takahashi, K., Inoue, S.-I., Okigaki, Y., Tomiyama, M., Hossain, M. A., Shimazaki, K.-I., Murata, Y., & Kinoshita, T. (2011) · Journal of Plant Research · doi:10.1007/s10265-011-0426-x · Cited by 83
“Mg-chelatase complex is involved in the biosynthetic pathway of chlorophyll, catalyzing the insertion of Mg 2? into protoporphyrin IX to form Mg-protoporphyrin IX (Gibson et al. 1995; Willows et al. 1996; Huang and Li 2009).”
InfluentialCampbell, B. W., Mani, D., Curtin, S. J., Slattery, R. A., Michno, J.-M., Ort, D. R., Schaus, P. J., Palmer, R. G., Orf, J. H., & Stupar, R. M. (2015) · G3 Genes|Genomes|Genetics · doi:10.1534/g3.114.015255 · Cited by 69
Evolution of enzymes and pathways for the biosynthesis of cofactors
Holliday, G. L., Thornton, J. M., Marquet, A., Smith, A. G., Rébeillé, F., Mendel, R., Schubert, H. L., Lawrence, A. D., & Warren, M. J. (2007) · Natural Product Reports · doi:10.1039/b703107f · Cited by 68
Metadata
- Authors
- Gibson, L. C., Willows, R. D., Kannangara, C. G., von Wettstein, D., & Hunter, C. N.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1995-03-14
- Volume
- 92
- Issue
- 6
- Pages
- 1941-1944
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2045304091
- Cited by
- 184
- References
- 0
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Magnesium-protoporphyrin chelatase of Rhodobacter sphaeroides: reconstitution of activity by combining the products of the bchH, -I, and -D genes expressed in Escherichia coli. https://app.cochat.ai/papers/10.1073/pnas.92.6.1941
- The paper
- Gibson, L. C., Willows, R. D., Kannangara, C. G., von Wettstein, D., & Hunter, C. N. (1995). Magnesium-protoporphyrin chelatase of Rhodobacter sphaeroides: reconstitution of activity by combining the products of the bchH, -I, and -D genes expressed in Escherichia coli. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.92.6.1941
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
