The DOI matches its Crossref record.
Stimulation of new bone formation by direct transfer of osteogenic plasmid genes.
Open accessAbstract
Degradable matrices containing expression plasmid DNA [gene-activated matrices (GAMs)] were implanted into segmental gaps created in the adult rat femur. Implantation of GAMs containing beta-galactosidase or luciferase plasmids led to DNA uptake and functional enzyme expression by repair cells (granulation tissue) growing into the gap. Implantation of a GAM containing either a bone morphogenetic protein-4 plasmid or a plasmid coding for a fragment of parathyroid hormone (amino acids 1-34) resulted in a biological response of new bone filling the gap. Finally, implantation of a two-plasmid GAM encoding bone morphogenetic protein-4 and the parathyroid hormone fragment, which act synergistically in vitro, caused new bone to form faster than with either factor alone. These studies demonstrate for the first time that repair cells (fibroblasts) in bone can be genetically manipulated in vivo. While serving as a useful tool to study the biology of repair fibroblasts and the wound healing response, the GAM technology may also have wide therapeutic utility.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
THE ROLE OF GROWTH FACTORS IN THE REPAIR OF BONE: BIOLOGY AND CLINICAL APPLICATIONS
LIEBERMAN, J. R., DALUISKI, A., & EINHORN, T. A. (2002) · The Journal of Bone and Joint Surgery-American Volume · doi:10.2106/00004623-200206000-00022 · Cited by 932
Peng, H., Wright, V., Usas, A., Gearhart, B., Shen, H.-C., Cummins, J., & Huard, J. (2002) · Journal of Clinical Investigation · doi:10.1172/jci15153 · Cited by 733
Strategies for controlled delivery of growth factors and cells for bone regeneration
Vo, T. N., Kasper, F. K., & Mikos, A. G. (2012) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.01.016 · Cited by 666
LIEBERMAN, J. R., DALUISKI, A., STEVENSON, S., JOLLA, L., WU, L., McALLISTER, P., LEE, Y. P., KABO, J. M., FINERMAN, G. A. M., BERK, A. J., et al. (1999) · The Journal of Bone & Joint Surgery · doi:10.2106/00004623-199907000-00002 · Cited by 527
Hubbell, J. A. (1999) · Current Opinion in Biotechnology · doi:10.1016/s0958-1669(99)80021-4 · Cited by 473
Metadata
- Authors
- Fang, J., Zhu, Y. Y., Smiley, E., Bonadio, J., Rouleau, J. P., Goldstein, S. A., McCauley, L. K., Davidson, B. L., & Roessler, B. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1996-06-11
- Volume
- 93
- Issue
- 12
- Pages
- 5753-5758
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2083164614
- Cited by
- 495
- References
- 0
- FWCI
- 15.84
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Stimulation of new bone formation by direct transfer of osteogenic plasmid genes. https://app.cochat.ai/papers/10.1073/pnas.93.12.5753
- The paper
- Fang, J., Zhu, Y. Y., Smiley, E., Bonadio, J., Rouleau, J. P., Goldstein, S. A., McCauley, L. K., Davidson, B. L., & Roessler, B. J. (1996). Stimulation of new bone formation by direct transfer of osteogenic plasmid genes. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.93.12.5753
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
