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The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins.
Open accessAbstract
Although transcription and pre-mRNA processing are colocalized in eukaryotic nuclei, molecules linking these processes have not previously been described. We have identified four novel rat proteins by their ability to interact with the repetitive C-terminal domain (CTD) of RNA polymerase II in a yeast two-hybrid assay. A yeast homolog of one of the rat proteins has also been shown to interact with the CTD. These CTD-binding proteins are all similar to the SR (serine/arginine-rich) family of proteins that have been shown to be involved in constitutive and regulated splicing. In addition to alternating Ser-Arg domains, these proteins each contain discrete N-terminal or C-terminal CTD-binding domains. We have identified SR-related proteins in a complex that can be immunoprecipitated from nuclear extracts with antibodies directed against RNA polymerase II. In addition, in vitro splicing is inhibited either by an antibody directed against the CTD or by wild-type but not mutant CTD peptides. Thus, these results suggest that the CTD and a set of CTD-binding proteins may act to physically and functionally link transcription and pre-mRNA processing.
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Cited by
A Cold-Inducible Coactivator of Nuclear Receptors Linked to Adaptive Thermogenesis
Puigserver, P., Wu, Z., Park, C. W., Graves, R., Wright, M., & Spiegelman, B. M. (1998) · Cell · doi:10.1016/s0092-8674(00)81410-5 · Cited by 3,864
Puigserver, P., & Spiegelman, B. M. (2003) · Endocrine Reviews · doi:10.1210/er.2002-0012 · Cited by 2,076
Vega, R. B., Huss, J. M., & Kelly, D. P. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.5.1868-1876.2000 · Cited by 1,195
Functional association between promoter structure and transcript alternative splicing
Cramer, P., Pesce, C. G., Baralle, F. E., & Kornblihtt, A. R. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.21.11456 · Cited by 344
RNA Polymerase II Targets Pre-mRNA Splicing Factors to Transcription Sites In Vivo
Misteli, T., & Spector, D. L. (1999) · Molecular Cell · doi:10.1016/s1097-2765(01)80002-2 · Cited by 326
Metadata
- Authors
- Yuryev, A., Patturajan, M., Litingtung, Y., Joshi, R. V., Gentile, C., Gebara, M., & Corden, J. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1996-07-09
- Volume
- 93
- Issue
- 14
- Pages
- 6975-6980
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2030932054
- Cited by
- 358
- References
- 0
- FWCI
- 11.01
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins. https://app.cochat.ai/papers/10.1073/pnas.93.14.6975
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- Yuryev, A., Patturajan, M., Litingtung, Y., Joshi, R. V., Gentile, C., Gebara, M., & Corden, J. L. (1996). The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.93.14.6975
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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