The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
A three-hybrid system to detect RNA-protein interactions in vivo.
Open accessAbstract
RNA-protein interactions are pivotal in fundamental cellular processes such as translation, mRNA processing, early development, and infection by RNA viruses. However, in spite of the central importance of these interactions, few approaches are available to analyze them rapidly in vivo. We describe a yeast genetic method to detect and analyze RNA-protein interactions in which the binding of a bifunctional RNA to each of two hybrid proteins activates transcription of a reporter gene in vivo. We demonstrate that this three-hybrid system enables the rapid, phenotypic detection of specific RNA-protein interactions. As examples, we use the binding of the iron regulatory protein 1 (IRP1) to the iron response element (IRE), and of HIV trans-activator protein (Tat) to the HIV trans-activation response element (TAR) RNA sequence. The three-hybrid assay we describe relies only on the physical properties of the RNA and protein, and not on their natural biological activities; as a result, it may have broad application in the identification of RNA-binding proteins and RNAs, as well as in the detailed analysis of their interactions.
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Cites
Regulating the fate of mRNA: the control of cellular iron metabolism
Klausner, R. D., Rouault, T. A., & Harford, J. B. (1993) · Cell · doi:10.1016/0092-8674(93)90046-s · Cited by 1,210
A protein linkage map of Escherichia coli bacteriophage T7
Bartel, P. L., Roecklein, J. A., SenGupta, D., & Fields, S. (1996) · Nature Genetics · doi:10.1038/ng0196-72 · Cited by 333
Self-citation
Cited by
A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae
Uetz, P., Giot, L., Cagney, G., Mansfield, T. A., Judson, R. S., Knight, J. R., Lockshon, D., Narayan, V., Srinivasan, M., Pochart, P., et al. (2000) · Nature · doi:10.1038/35001009 · Cited by 4,706
Self-citationRNA‐binding proteins and post‐transcriptional gene regulation
Glisovic, T., Bachorik, J. L., Yong, J., & Dreyfuss, G. (2008) · FEBS Letters · doi:10.1016/j.febslet.2008.03.004 · Cited by 1,398
Puig, S., Askeland, E., & Thiele, D. J. (2005) · Cell · doi:10.1016/j.cell.2004.11.032 · Cited by 382
Mazumder, B., Sampath, P., Seshadri, V., Maitra, R. K., DiCorleto, P. E., & Fox, P. L. (2003) · Cell · doi:10.1016/s0092-8674(03)00773-6 · Cited by 332
Glansdorff, N., Xu, Y., & Labedan, B. (2008) · Biology Direct · doi:10.1186/1745-6150-3-29 · Cited by 264
Metadata
- Authors
- SenGupta, D. J., Zhang, B., Kraemer, B., Pochart, P., Fields, S., & Wickens, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1996-08-06
- Volume
- 93
- Issue
- 16
- Pages
- 8496-8501
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2052108496
- Cited by
- 510
- References
- 2
- FWCI
- 10.27
- Type
- journal article
- Language
- en
- Open access
- green
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- SenGupta, D. J., Zhang, B., Kraemer, B., Pochart, P., Fields, S., & Wickens, M. (1996). A three-hybrid system to detect RNA-protein interactions in vivo. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.93.16.8496
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