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DNA strand annealing is promoted by the yeast Rad52 protein.
Open accessAbstract
The Saccharomyces cerevisiae RAD52 gene plays a pivotal role in genetic recombination. Here we demonstrate that yeast Rad52 is a DNA binding protein. To show that the interaction between Rad52 and DNA is direct and not mediated by other yeast proteins and to facilitate protein purification, a recombinant expression system was developed. The recombinant protein can bind both single- and double-stranded DNA and the addition of either Mg2+ or ATP does not enhance the binding of single-stranded DNA. Furthermore, a DNA binding domain was found in the evolutionary conserved N terminus of the protein. More importantly, we show that the protein stimulates DNA annealing even in the presence of a large excess of nonhomologous DNA. Rad52-promoted annealing follows second-order kinetics and the rate is 3500-fold faster than that of the spontaneous reaction. How this annealing activity relates to the genetic phenotype associated with rad52 mutant cells is discussed.
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Cited by
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
Pâques, F., & Haber, J. E. (1999) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.63.2.349-404.1999 · Cited by 2,120
Hastings, P. J., Ira, G., & Lupski, J. R. (2009) · PLoS Genetics · doi:10.1371/journal.pgen.1000327 · Cited by 818
DNA Repair Pathway Choices in CRISPR-Cas9-Mediated Genome Editing
Xue, C., & Greene, E. C. (2021) · Trends in Genetics · doi:10.1016/j.tig.2021.02.008 · Cited by 431
Regulation of Bacterial RecA Protein Function
Cox, M. M. (2007) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.1080/10409230701260258 · Cited by 417
Collaboration and competition between DNA double‐strand break repair pathways
Kass, E. M., & Jasin, M. (2010) · FEBS Letters · doi:10.1016/j.febslet.2010.07.057 · Cited by 326
Metadata
- Authors
- Mortensen, U. H., Bendixen, C., Sunjevaric, I., & Rothstein, R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1996-10
- Volume
- 93
- Issue
- 20
- Pages
- 10729-10734
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2035994249
- Cited by
- 472
- References
- 0
- FWCI
- 5.20
- Type
- journal article
- Language
- en
- Open access
- green
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- Mortensen, U. H., Bendixen, C., Sunjevaric, I., & Rothstein, R. (1996). DNA strand annealing is promoted by the yeast Rad52 protein. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.93.20.10729
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