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DNA transport by a type II topoisomerase: direct evidence for a two-gate mechanism.
Open accessAbstract
Recent biochemical and crystallographic results suggest that a type II DNA topoisomerase acts as an ATP-modulated clamp with two sets of jaws at opposite ends: a DNA-bound enzyme can admit a second DNA through one set of jaws; upon binding ATP, this DNA is passed through an enzyme-mediated opening in the first DNA and expelled from the enzyme through the other set of jaws. Experiments based on the introduction of reversible disulfide links across one dimer interface of yeast DNA topoisomerase II have confirmed this mechanism. The second DNA is found to enter the enzyme through the gate formed by the N-terminal parts of the enzyme and leave it through the gate close to the C termini.
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Citations
Cited by
DNA Topoisomerases: Structure, Function, and Mechanism
Champoux, J. J. (2001) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.70.1.369 · Cited by 2,743
Mechanism of action of eukaryotic topoisomerase II and drugs targeted to the enzyme
Burden, D. A., & Osheroff, N. (1998) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/s0167-4781(98)00132-8 · Cited by 503
DNA topoisomerases: harnessing and constraining energy to govern chromosome topology
Schoeffler, A. J., & Berger, J. M. (2008) · Quarterly Reviews of Biophysics · doi:10.1017/s003358350800468x · Cited by 490
Self-citationTopoisomerases as anticancer targets
Delgado, J. L., Hsieh, C.-M., Chan, N.-L., & Hiasa, H. (2018) · Biochemical Journal · doi:10.1042/bcj20160583 · Cited by 481
New Mechanistic and Functional Insights into DNA Topoisomerases
Chen, S. H., Chan, N.-L., & Hsieh, T.-S. (2013) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-061809-100002 · Cited by 369
Metadata
- Authors
- Roca, J., Berger, J. M., Harrison, S. C., & Wang, J. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1996-04-30
- Volume
- 93
- Issue
- 9
- Pages
- 4057-4062
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2083921717
- Cited by
- 194
- References
- 0
- FWCI
- 3.34
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: DNA transport by a type II topoisomerase: direct evidence for a two-gate mechanism. https://app.cochat.ai/papers/10.1073/pnas.93.9.4057
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- Roca, J., Berger, J. M., Harrison, S. C., & Wang, J. C. (1996). DNA transport by a type II topoisomerase: direct evidence for a two-gate mechanism. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.93.9.4057
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
