The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
A “knockdown” mutation created by cis-element gene targeting reveals the dependence of erythroid cell maturation on the level of transcription factor GATA-1
Open accessAbstract
The hematopoietic-restricted transcription factor GATA-1 is required for both mammalian erythroid cell and megakaryocyte differentiation. To define the mechanisms governing its transcriptional regulation, we replaced upstream sequences including a DNase I hypersensitive (HS) region with a neomycin-resistance cassette by homologous recombination in mouse embryonic stem cells and generated mice either harboring this mutation (neoΔHS) or lacking the selection cassette (ΔneoΔHS). Studies of the consequences of these targeted mutations provide novel insights into GATA-1 function in erythroid cells. First, the neoΔHS mutation leads to a marked impairment in the rate or efficiency of erythroid cell maturation due to a modest (4- to 5-fold) decrease in GATA-1 expression. Hence, erythroid differentiation is dose-dependent with respect to GATA-1. Second, since expression of GATA-1 from the ΔneoΔHS allele in erythroid cells is largely restored, transcription interference imposed by the introduced cassette must account for the “knockdown” effect of the mutation. Finally, despite the potency of the upstream sequences in conferring high-level, developmentally appropriate expression of transgenes in mice, other cis-regulatory elements within the GATA-1 compensate for its absence in erythroid cells. Our work illustrates the usefulness of targeted mutations to create knockdown mutations that may uncover important quantitative contributions of gene function not revealed by conventional knockouts.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Pevny, L., Simon, M. C., Robertson, E., Klein, W. H., Tsai, S.-F., D'Agati, V., Orkin, S. H., & Costantini, F. (1991) · Nature · doi:10.1038/349257a0 · Cited by 1,308
Self-citationTsang, A. P., Visvader, J. E., Turner, C. A., Fujiwara, Y., Yu, C., Weiss, M. J., Crossley, M., & Orkin, S. H. (1997) · Cell · doi:10.1016/s0092-8674(00)80318-9 · Cited by 712
Self-citationWeiss, M. J., Keller, G., & Orkin, S. H. (1994) · Genes & Development · doi:10.1101/gad.8.10.1184 · Cited by 541
Self-citationMerika, M., & Orkin, S. H. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.5.2437 · Cited by 462
Self-citationKulessa, H., Frampton, J., & Graf, T. (1995) · Genes & Development · doi:10.1101/gad.9.10.1250 · Cited by 418
Transcriptional activation and DNA binding by the erythroid factor GF-1/NF-E1/Eryf 1.
Martin, D. I., & Orkin, S. H. (1990) · Genes & Development · doi:10.1101/gad.4.11.1886 · Cited by 409
Self-citationTsai, S. F., Strauss, E., & Orkin, S. H. (1991) · Genes & Development · doi:10.1101/gad.5.6.919 · Cited by 327
Self-citationCrossley, M., Whitelaw, E., Perkins, A., Williams, G., Fujiwara, Y., & Orkin, S. H. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.4.1695 · Cited by 222
Osada, H., Grutz, G., Axelson, H., Forster, A., & Rabbitts, T. H. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.21.9585 · Cited by 192
Visvader, J. E., Crossley, M., Hill, J., Orkin, S. H., & Adams, J. M. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.2.634 · Cited by 151
Self-citation
Cited by
Pten Dose Dictates Cancer Progression in the Prostate
Trotman, L. C., Niki, M., Dotan, Z. A., Koutcher, J. A., Di Cristofano, A., Xiao, A., Khoo, A. S., Roy-Burman, P., Greenberg, N. M., Dyke, T. V., et al. (2003) · PLoS Biology · doi:10.1371/journal.pbio.0000059 · Cited by 676
Shivdasani, R. A., Fujiwara, Y., McDevitt, M. A., & Orkin, S. H. (1997) · The EMBO Journal · doi:10.1093/emboj/16.13.3965 · Cited by 674
Self-citationSox2 deficiency causes neurodegeneration and impaired neurogenesis in the adult mouse brain
Ferri, A. L. M., Cavallaro, M., Braida, D., Di Cristofano, A., Canta, A., Vezzani, A., Ottolenghi, S., Pandolfi, P. P., Sala, M., DeBiasi, S., et al. (2004) · Development · doi:10.1242/dev.01204 · Cited by 665
Disruption of differentiation in human cancer: AML shows the way
Tenen, D. G. (2003) · Nature Reviews Cancer · doi:10.1038/nrc989 · Cited by 612
Subtle variations in Pten dose determine cancer susceptibility
Alimonti, A., Carracedo, A., Clohessy, J. G., Trotman, L. C., Nardella, C., Egia, A., Salmena, L., Sampieri, K., Haveman, W. J., Brogi, E., et al. (2010) · Nature Genetics · doi:10.1038/ng.556 · Cited by 580
Metadata
- Authors
- McDevitt, M. A., Shivdasani, R. A., Fujiwara, Y., Yang, H., & Orkin, S. H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-06-24
- Volume
- 94
- Issue
- 13
- Pages
- 6781-6785
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1983631422
- Cited by
- 227
- References
- 11
- FWCI
- 3.71
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A “knockdown” mutation created by cis-element gene targeting reveals the dependence of erythroid cell maturation on the level of transcription factor GATA-1. https://app.cochat.ai/papers/10.1073/pnas.94.13.6781
- The paper
- McDevitt, M. A., Shivdasani, R. A., Fujiwara, Y., Yang, H., & Orkin, S. H. (1997). A “knockdown” mutation created by cis-element gene targeting reveals the dependence of erythroid cell maturation on the level of transcription factor GATA-1. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.13.6781
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
