The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
β-Catenin mutations in cell lines established from human colorectal cancers
Open accessAbstract
β-catenin has functions as both an adhesion and a signaling molecule. Disruption of these functions through mutations of the β-catenin gene ( CTNNB1 ) may be important in the development of colorectal tumors. We examined the entire coding sequence of β-catenin by reverse transcriptase–PCR (RT-PCR) and direct sequencing of 23 human colorectal cancer cell lines from 21 patients. In two cell lines, there was apparent instability of the β-catenin mRNA. Five different mutations (26%) were found in the remaining 21cell lines (from 19 patients). A three-base deletion (codon 45) was identified in the cell line HCT 116, whereas cell lines SW 48, HCA 46, CACO 2, and Colo 201 each contained single-base missense mutations (codons 33, 183, 245, and 287, respectively). All 23 cell lines had full-length β-catenin protein that was detectable by Western blotting and that coprecipitated with E-cadherin. In three of the cell lines with CTNNB1 mutations, complexes of β-catenin with α-catenin and APC were detectable. In SW48 and HCA 46, however, we did not detect complexes of β-catenin protein with α-catenin and APC, respectively. These results show that selection of CTNNB1 mutations occurs in up to 26% of colorectal cancers from which cell lines are derived. In these cases, mutation selection is probably for altered β-catenin function, which may significantly alter intracellular signaling and intercellular adhesion and may serve as a complement to APC mutations in the early stages of tumorigenesis.
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Cites
Activation of β-Catenin-Tcf Signaling in Colon Cancer by Mutations in β-Catenin or APC
Morin, P. J., Sparks, A. B., Korinek, V., Barker, N., Clevers, H., Vogelstein, B., & Kinzler, K. W. (1997) · Science · doi:10.1126/science.275.5307.1787 · Cited by 3,942
Functional interaction of β-catenin with the transcription factor LEF-1
Behrens, J., von Kries, J. P., Kühl, M., Bruhn, L., Wedlich, D., Grosschedl, R., & Birchmeier, W. (1996) · Nature · doi:10.1038/382638a0 · Cited by 2,912
XTcf-3 Transcription Factor Mediates β-Catenin-Induced Axis Formation in Xenopus Embryos
Molenaar, M., van de Wetering, M., Oosterwegel, M., Peterson-Maduro, J., Godsave, S., Korinek, V., Roose, J., Destrée, O., & Clevers, H. (1996) · Cell · doi:10.1016/s0092-8674(00)80112-9 · Cited by 1,824
Current Protocols in Molecular Biology
Teplitz, R. (1989) · JAMA: The Journal of the American Medical Association · doi:10.1001/jama.1989.03420090114049 · Cited by 1,803
Binding of GSK3β to the APC-β-Catenin Complex and Regulation of Complex Assembly
Rubinfeld, B., Albert, I., Porfiri, E., Fiol, C., Munemitsu, S., & Polakis, P. (1996) · Science · doi:10.1126/science.272.5264.1023 · Cited by 1,422
Ozawa, M., Baribault, H., & Kemler, R. (1989) · The EMBO Journal · doi:10.1002/j.1460-2075.1989.tb03563.x · Cited by 1,396
Yost, C., Torres, M., Miller, J. R., Huang, E., Kimelman, D., & Moon, R. T. (1996) · Genes & Development · doi:10.1101/gad.10.12.1443 · Cited by 1,165
Munemitsu, S., Albert, I., Souza, B., Rubinfeld, B., & Polakis, P. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.7.3046 · Cited by 1,021
A repeating amino acid motif shared by proteins with diverse cellular roles
Peifer, M., Berg, S., & Reynolds, A. B. (1994) · Cell · doi:10.1016/0092-8674(94)90353-0 · Cited by 648
A Homolog of the armadillo Protein in Drosophila (Plakoglobin) Associated with E-Cadherin
McCrea, P. D., Turck, C. W., & Gumbiner, B. (1991) · Science · doi:10.1126/science.1962194 · Cited by 623
Cited by
Caught up in a Wnt storm: Wnt signaling in cancer
Giles, R. H., van Es, J. H., & Clevers, H. (2003) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/s0304-419x(03)00005-2 · Cited by 1,482
Somatic mutations of the β-catenin gene are frequent in mouse and human hepatocellular carcinomas
Coste, A. D. L., Romagnolo, B., Billuart, P., Renard, C.-A., Buendia, M.-A., Soubrane, O., Fabre, M., Chelly, J., Beldjord, C., Kahn, A., et al. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.15.8847 · Cited by 1,121
Mann, B., Gelos, M., Siedow, A., Hanski, M. L., Gratchev, A., Ilyas, M., Bodmer, W. F., Moyer, M. P., Riecken, E. O., Buhr, H. J., et al. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.4.1603 · Cited by 663
Self-citationColorectal cancer and genetic alterations in the Wnt pathway
Segditsas, S., & Tomlinson, I. (2006) · Oncogene · doi:10.1038/sj.onc.1210059 · Cited by 591
Self-citationThe Wnt/Wg Signal Transducer β-Catenin Controls Fibronectin Expression
Gradl, D., Kühl, M., & Wedlich, D. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.8.5576 · Cited by 355
Metadata
- Authors
- Ilyas, M., Tomlinson, I. P. M., Rowan, A., Pignatelli, M., & Bodmer, W. F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-09-16
- Volume
- 94
- Issue
- 19
- Pages
- 10330-10334
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2053086049
- Cited by
- 494
- References
- 24
- FWCI
- 6.97
- Type
- journal article
- Language
- en
- Open access
- green
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- Ilyas, M., Tomlinson, I. P. M., Rowan, A., Pignatelli, M., & Bodmer, W. F. (1997). β-Catenin mutations in cell lines established from human colorectal cancers. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.19.10330
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