The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Cloning and characterization of human protease-activated receptor 4
Open accessAbstract
Protease-activated receptors 1–3 (PAR1, PAR2, and PAR3) are members of a unique G protein-coupled receptor family. They are characterized by a tethered peptide ligand at the extracellular amino terminus that is generated by minor proteolysis. A partial cDNA sequence of a fourth member of this family (PAR4) was identified in an expressed sequence tag database, and the full-length cDNA clone has been isolated from a lymphoma Daudi cell cDNA library. The ORF codes for a seven transmembrane domain protein of 385 amino acids with 33% amino acid sequence identity with PAR1, PAR2, and PAR3. A putative protease cleavage site (Arg-47/Gly-48) was identified within the extracellular amino terminus. COS cells transiently transfected with PAR4 resulted in the formation of intracellular inositol triphosphate when treated with either thrombin or trypsin. A PAR4 mutant in which the Arg-47 was replaced with Ala did not respond to thrombin or trypsin. A hexapeptide (GYPGQV) representing the newly exposed tethered ligand from the amino terminus of PAR4 after proteolysis by thrombin activated COS cells transfected with either wild-type or the mutant PAR4. Northern blot showed that PAR4 mRNA was expressed in a number of human tissues, with high levels being present in lung, pancreas, thyroid, testis, and small intestine. By fluorescence in situ hybridization, the human PAR4 gene was mapped to chromosome 19p12.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Basic local alignment search tool
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990) · Journal of Molecular Biology · doi:10.1006/jmbi.1990.9999 · Cited by 98,669
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Vu, T.-K. H., Hung, D. T., Wheaton, V. I., & Coughlin, S. R. (1991) · Cell · doi:10.1016/0092-8674(91)90261-v · Cited by 2,970
[5] Rapid and sensitive sequence comparison with FASTP and FASTA
Pearson, W. R. (1990) · Methods in Enzymology · doi:10.1016/0076-6879(90)83007-v · Cited by 1,946
The PROSITE database, its status in 1995
Bairoch, A. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.1.189 · Cited by 1,924
Molecular cloning of a potential proteinase activated receptor.
Nystedt, S., Emilsson, K., Wahlestedt, C., & Sundelin, J. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.20.9208 · Cited by 886
Protease-activated receptor 3 is a second thrombin receptor in humans
Ishihara, H., Connolly, A. J., Zeng, D., Kahn, M. L., Wu Zheng, Y., Timmons, C., Tram, T., & Coughlin, S. R. (1997) · Nature · doi:10.1038/386502a0 · Cited by 865
Sequence Alignment of the G-Protein Coupled Receptor Superfamily
PROBST, W. C., SNYDER, L. A., SCHUSTER, D. I., BROSIUS, J., & SEALFON, S. C. (1992) · DNA and Cell Biology · doi:10.1089/dna.1992.11.1 · Cited by 845
Role of the thrombin receptor in development and evidence for a second receptor
Connolly, A. J., Ishihara, H., Kahn, M. L., Farese, R. V., & Coughlin, S. R. (1996) · Nature · doi:10.1038/381516a0 · Cited by 505
Ishii, K., Chen, J., Ishii, M., Koch, W. J., Freedman, N. J., Lefkowitz, R. J., & Coughlin, S. R. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)42230-7 · Cited by 223
Cited by
Protease-Activated Receptors: Contribution to Physiology and Disease
OSSOVSKAYA, V. S., & BUNNETT, N. W. (2004) · Physiological Reviews · doi:10.1152/physrev.00028.2003 · Cited by 1,078
Activation of Platelet Function Through G Protein–Coupled Receptors
Offermanns, S. (2006) · Circulation Research · doi:10.1161/01.res.0000251742.71301.16 · Cited by 725
Proteinase-Activated Receptors
Macfarlane, S. R., Seatter, M. J., Kanke, T., Hunter, G. D., & Plevin, R. (2001) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01493-5 · Cited by 641
Structure, function and pathophysiology of protease activated receptors
Adams, M. N., Ramachandran, R., Yau, M.-K., Suen, J. Y., Fairlie, D. P., Hollenberg, M. D., & Hooper, J. D. (2011) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2011.01.003 · Cited by 370
Clinical Aspects of Platelet Inhibitors and Thrombus Formation
Meadows, T. A., & Bhatt, D. L. (2007) · Circulation Research · doi:10.1161/01.res.0000264509.36234.51 · Cited by 351
Metadata
- Authors
- Xu, W.-F., Andersen, H., Whitmore, T. E., Presnell, S. R., Yee, D. P., Ching, A., Gilbert, T., Davie, E. W., & Foster, D. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1998-06-09
- Volume
- 95
- Issue
- 12
- Pages
- 6642-6646
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1964499190
- Cited by
- 817
- References
- 24
- FWCI
- 34.14
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Cloning and characterization of human protease-activated receptor 4. https://app.cochat.ai/papers/10.1073/pnas.95.12.6642
- The paper
- Xu, W.-F., Andersen, H., Whitmore, T. E., Presnell, S. R., Yee, D. P., Ching, A., Gilbert, T., Davie, E. W., & Foster, D. C. (1998). Cloning and characterization of human protease-activated receptor 4. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.95.12.6642
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
