Efficient recruitment of TFIIB and CBP-RNA polymerase II holoenzyme by an interferon-β enhanceosome in vitro
Open accessAbstract
The transcriptional activity of an in vitro assembled human interferon-β gene enhanceosome is highly synergistic. This synergy requires five distinct transcriptional activator proteins (ATF2/c-JUN, interferon regulatory factor 1, and p50/p65 of NF-κB), the high mobility group protein HMG I(Y), and the correct alignment of protein-binding sites on the face of the DNA double helix. Here, we investigate the mechanisms of enhanceosome-dependent transcriptional synergy during preinitiation complex assembly in vitro . We show that the stereospecific assembly of the enhanceosome is critical for the efficient recruitment of TFIIB into a template-committed TFIID-TFIIA-USA (upstream stimulatory activity complex) and for the subsequent recruitment of the RNA polymerase II holoenzyme complex. In addition, we provide evidence that recruitment of the holoenzyme by the enhanceosome is due, at least in part, to interactions between the enhanceosome and the transcriptional coactivator CREB, cAMP responsive element binding protein (CBP). These studies reveal a unique role of enhanceosomes in the cooperative assembly of the transcription machinery on the human interferon-β promoter.
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P300 and CBP serve as scaffolds that connect RNA polymerase II and RNAP-associated general transcription factors to chromatin.
“P300 and CBP also serve as scaffolds that connect RNA polymerase II (RNAP) and RNAP-associated “general transcription factors” to the chromatin [64, 65].”
From Transcriptional enhancers and their communication with gene promoters (2021) · cites this paper as [65]
The Results and Discussion (page unavailable) show that interactions between CBP and the IFN-β enhanceosome help recruit an RNA polymerase II holoenzyme containing general transcription factors to a promoter. The paper does not establish the same role for p300 or show that either protein acts as a scaffold connecting those factors to chromatin; its experiments used an immobilized DNA template in vitro. (LLM, conf=0.85)
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Lin, R., Heylbroeck, C., Pitha, P. M., & Hiscott, J. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.5.2986 · Cited by 915 · see citing works
Wathelet, M. G., Lin, C. H., Parekh, B. S., Ronco, L. V., Howley, P. M., & Maniatis, T. (1998) · Molecular Cell · doi:10.1016/s1097-2765(00)80051-9 · Cited by 730 · see citing works
Self-citationThe enhanceosome and transcriptional synergy.
Carey, M. (1998) · Cell · doi:10.1016/s0092-8674(00)80893-4 · Cited by 553 · see citing works
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Research works printed but not linked: 3
- [3]Maniatis, T., Falvo, J. V., Kim, T. H., Kim, T. K., Lin, C. H., Parekh, B. S. & Wathelet, M. G. (1998) Cold Spring Harbor Symp. Quant. Biol. 63, in press.
- [10]Kim, T. K. & Roeder, R. G. (1994) Proc. Natl. Acad. Sci. USA 91, 4170-4174.
- [21]Gerritsen, M. E., Williams, A. J., Neish, A. S., Moore, S., Shi, Y. & Collins, T. (1997) Proc. Natl. Acad. Sci. USA 94, 2927-2932.
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CBP/p300 in cell growth, transformation, and development
Goodman, R. H., & Smolik, S. (2000) · Genes & Development · doi:10.1101/gad.14.13.1553 · Cited by 1,778 · see citing works
Rosenfeld, M. G., Lunyak, V. V., & Glass, C. K. (2006) · Genes & Development · doi:10.1101/gad.1424806 · Cited by 952 · see citing works
Evidence for additive and synergistic action of mammalian enhancers during cell fate determination
Choi, J., Lysakovskaia, K., Stik, G., Demel, C., Söding, J., Tian, T. V., Graf, T., & Cramer, P. (2021) · eLife · doi:10.7554/elife.65381 · Cited by 117 · see citing works
“Additionally or alternatively, enhancer synergy may also stem from sequential cooperation if different enhancers target different steps of transcription such as initiation and elongation (Beagrie and Pombo, 2016; Haberle and Stark, 2018; Henriques et al., 2018; Kim et al., 1998; Sawado et al., 2003; Spicuglia et al.,…”
BackgroundTranscriptional enhancers and their communication with gene promoters
Ray-Jones, H., & Spivakov, M. (2021) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-021-03903-w · Cited by 81 · see citing works
- Authors
- Kim, T. K., Kim, T. H., & Maniatis, T.
- Affiliations
- Harvard University (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1998-10-13
- Volume
- 95
- Issue
- 21
- Pages
- 12191-12196
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC22807
- OpenAlex
- W2005725217
- Cited by
- 109
- References
- 3
- Citations by year
- 2026: 1 · 2022: 1 · 2021: 7 · 2020: 2 · 2019: 3 · 2018: 2 · 2017: 1 · 2015: 2 · 2013: 1 · 2012: 3
- FWCI
- 4.78
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Damon Runyon Cancer Research Foundation
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (R01AI020642, AI20642)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- interferon-beta, enhanceosome, transcriptional synergy, preinitiation complex assembly, RNA polymerase II holoenzyme, TFIID, CREB, CBP, transcriptional coactivator, HMGA1, IRF1, NF-κB
- MeSH terms
- Enhancer Elements, Genetic, HeLa Cells, Humans, Promoter Regions, Genetic, RNA Polymerase II, Transcription Factors, Interferon-beta, Cyclic AMP Response Element-Binding Protein, Holoenzymes, Transcription Factor TFIIB
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