The DOI matches its Crossref record; 33 of the 34 references it cites were found in a registry or an index.
AtPCS1, a phytochelatin synthase from Arabidopsis : Isolation and in vitro reconstitution
Open accessAbstract
Phytochelatins, a class of posttranslationally synthesized peptides, play a pivotal role in heavy metal, primarily Cd2+, tolerance in plants and fungi by chelating these substances and decreasing their free concentrations. Derived from glutathione and related thiols by the action of gamma-glutamylcysteine dipeptidyl transpeptidases (phytochelatin synthases; EC 2.3.2.15), phytochelatins consist of repeating units of gamma-glutamylcysteine followed by a C-terminal Gly, Ser, or beta-Ala residue [poly-(gamma-Glu-Cys)n-Xaa]. Here we report the suppression cloning of a cDNA (AtPCS1) from Arabidopsis thaliana encoding a 55-kDa soluble protein that enhances heavy-metal tolerance and elicits Cd2+-activated phytochelatin accumulation when expressed in Saccharomyces cerevisiae. On the basis of these properties and the sufficiency of immunoaffinity-purified epitope-tagged AtPCS1 polypeptide for high rates of Cd2+-activated phytochelatin synthesis from glutathione in vitro, AtPCS1 is concluded to encode the enzyme phytochelatin synthase.
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“each of these gene products is not only necessary but also sufficient for the elaboration of PCs.”
From Mechanism of Heavy Metal Ion Activation of Phytochelatin (PC) Synthase (2000) · cites this paper as [13]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
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“Isolated from Arabidopsis,S. pombe, and wheat, these genes, designatedAtPCS1, SpPCS, and TaPCS1, respectively, encode 40–50% sequence-similar 50–55-kDa polypeptides active in the synthesis of PCs from GSH (11-13”
From Mechanism of Heavy Metal Ion Activation of Phytochelatin (PC) Synthase (2000) · cites this paper as [13]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
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“heterologously expressed AtPCS1-FLAG was 30- to 50-fold immunopurified from the soluble fraction of pYES3-AtPCS1::FLAG-transformed S. cerevisiae strain DTY167 to yield a single anti-FLAG antibody-reactive, M r = 58,000 polypeptide species”
From Mechanism of Heavy Metal Ion Activation of Phytochelatin (PC) Synthase (2000) · cites this paper as [13]
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“heterologous expression ofAtPCS1 in Saccharomyces cerevisiae, an organism that lacks PCS homologs and does not otherwise synthesize appreciable amounts of PCs, confers increased heavy metal tolerance and elicits Cd2+-dependent intracellular PC accumulation (13”
From Mechanism of Heavy Metal Ion Activation of Phytochelatin (PC) Synthase (2000) · cites this paper as [13]
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Metadata
- Authors
- Vatamaniuk, O. K., Mari, S., Lu, Y.-P., & Rea, P. A.
- Affiliations
- University of Pennsylvania (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1999-06-08
- Volume
- 96
- Issue
- 12
- Pages
- 7110-7115
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC22073
- OpenAlex
- W2096818569
- Cited by
- 410
- References
- 34
- Citations by year
- 2026: 6 · 2025: 4 · 2024: 6 · 2023: 8 · 2022: 13 · 2021: 9 · 2020: 11 · 2019: 11 · 2018: 11 · 2017: 8 · 2016: 10 · 2015: 22 · 2014: 8 · 2013: 12 · 2012: 19
- FWCI
- 29.02
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
- Keywords
- phytochelatin synthase, phytochelatins, cadmium tolerance, heavy metal tolerance, glutathione, Arabidopsis thaliana, Saccharomyces cerevisiae expression, metal chelation
- MeSH terms
- Amino Acid Sequence, Molecular Sequence Data, Plant Proteins, Saccharomyces cerevisiae, Sequence Alignment, Arabidopsis, Aminoacyltransferases
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