Cited by 410Plant Stress Responses and ToleranceHeavy metals in environmentHeavy Metal Exposure and Toxicity

The DOI matches its Crossref record; 33 of the 34 references it cites were found in a registry or an index.

AtPCS1, a phytochelatin synthase from Arabidopsis : Isolation and in vitro reconstitution

Open access

Vatamaniuk, Olena K., Mari, Stéphane, Lu, Yu-Ping, Rea, Philip A.·1999·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.96.12.7110PMC PMC22073

Open this paper in CoChat — free account

Abstract

Phytochelatins, a class of posttranslationally synthesized peptides, play a pivotal role in heavy metal, primarily Cd2+, tolerance in plants and fungi by chelating these substances and decreasing their free concentrations. Derived from glutathione and related thiols by the action of gamma-glutamylcysteine dipeptidyl transpeptidases (phytochelatin synthases; EC 2.3.2.15), phytochelatins consist of repeating units of gamma-glutamylcysteine followed by a C-terminal Gly, Ser, or beta-Ala residue [poly-(gamma-Glu-Cys)n-Xaa]. Here we report the suppression cloning of a cDNA (AtPCS1) from Arabidopsis thaliana encoding a 55-kDa soluble protein that enhances heavy-metal tolerance and elicits Cd2+-activated phytochelatin accumulation when expressed in Saccharomyces cerevisiae. On the basis of these properties and the sufficiency of immunoaffinity-purified epitope-tagged AtPCS1 polypeptide for high rates of Cd2+-activated phytochelatin synthesis from glutathione in vitro, AtPCS1 is concluded to encode the enzyme phytochelatin synthase.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1073/pnas.96.12.7110

Cites

33 of 33 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
An Algorithm for Least-Squares Estimation of Nonlinear Parameters
Marquardt, D. W. (1963) · Journal of the Society for Industrial and Applied Mathematics · doi:10.1137/0111030 · Cited by 30,728
Tissue sulfhydryl groups
Ellman, G. L. (1959) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(59)90090-6 · Cited by 26,824
CLUSTAL: a package for performing multiple sequence alignment on a microcomputer
Higgins, D. G., & Sharp, P. M. (1988) · Gene · doi:10.1016/0378-1119(88)90330-7 · Cited by 3,394
Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range
Udenfriend, S., Stein, S., Böhlen, P., Dairman, W. M., Leimgruber, W., & Weigele, M. (1972) · Science · doi:10.1126/science.178.4063.871 · Cited by 2,639
METALLOTHIONEIN
Hamer, D. H. (1986) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.55.070186.004405 · Cited by 1,655
Metal ion-catalyzed oxidation of proteins: Biochemical mechanism and biological consequences
Stadtman, E. R. (1990) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(90)90006-5 · Cited by 1,160
Heavy metal detoxification in higher plants - a review
Zenk, M. H. (1996) · Gene · doi:10.1016/s0378-1119(96)00422-2 · Cited by 1,003
Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific γ-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase)
Grill, E., Löffler, S., Winnacker, E., & Zenk, M. H. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.18.6838 · Cited by 916
Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast
Clemens, S., Kim, E. J., Neumann, D., & Schroeder, J. I. (1999) · The EMBO Journal · doi:10.1093/emboj/18.12.3325 · Cited by 660

Show all 34 references

Research works printed but not linked: 5

D Voet, J G Voet Biochemistry (Wiley, 2nd Ed., New York, 1995).
J Zhou, P B Goldsbrough Plant Cell 6, 875–884 (1994).
M Minet, M Dufour, F Lacronte Plant J 2, 417–422 (1992). · DOI
Ha S.-B Smith A. P. Howden R. Dietrich W. M. Bugg S. O’Connell M. J. Goldsbrough P. B. & Cobbett C. S. (1999) Plant Cell in press.
Clemens S. Kim E. J. Neumann D. & Schroeder J. I. (1999) EMBO J. in press.

Sorted by citations

Cellular mechanisms for heavy metal detoxification and tolerance
Hall, J. L. (2002) · Journal of Experimental Botany · doi:10.1093/jexbot/53.366.1 · Cited by 2,968
P HYTOCHELATINS AND M ETALLOTHIONEINS : Roles in Heavy Metal Detoxification and Homeostasis
Cobbett, C., & Goldsbrough, P. (2002) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.53.100301.135154 · Cited by 2,518
Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants
Clemens, S. (2006) · Biochimie · doi:10.1016/j.biochi.2006.07.003 · Cited by 2,098
Heavy metals toxicity in plants: An overview on the role of glutathione and phytochelatins in heavy metal stress tolerance of plants
Yadav, S. K. (2009) · South African Journal of Botany · doi:10.1016/j.sajb.2009.10.007 · Cited by 1,611
Phytochelatins and Their Roles in Heavy Metal Detoxification
Cobbett, C. S. (2000) · PLANT PHYSIOLOGY · doi:10.1104/pp.123.3.825 · Cited by 1,430
Molecular mechanisms of plant metal tolerance and homeostasis
Clemens, S. (2001) · Planta · doi:10.1007/s004250000458 · Cited by 1,417
The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress
Sharma, S. S., & Dietz, K. (2006) · Journal of Experimental Botany · doi:10.1093/jxb/erj073 · Cited by 1,380
A long way ahead: understanding and engineering plant metal accumulation
Clemens, S., Palmgren, M. G., & Krämer, U. (2002) · Trends in Plant Science · doi:10.1016/s1360-1385(02)02295-1 · Cited by 1,265
Phytoextraction of Toxic Metals
Lasat, M. M. (2002) · Journal of Environmental Quality · doi:10.2134/jeq2002.1090 · Cited by 1,224
Cadmium toxicity in plants
Benavides, M. P., Gallego, S. M., & Tomaro, M. L. (2005) · Brazilian Journal of Plant Physiology · doi:10.1590/s1677-04202005000100003 · Cited by 1,208
Phytoremediation of toxic elemental and organic pollutants
Meagher, R. B. (2000) · Current Opinion in Plant Biology · doi:10.1016/s1369-5266(99)00054-0 · Cited by 902
Comparison of mercury, lead and arsenic with respect to genotoxic effects on plant systems and the development of genetic tolerance
Patra, M., Bhowmik, N., Bandopadhyay, B., & Sharma, A. (2004) · Environmental and Experimental Botany · doi:10.1016/j.envexpbot.2004.02.009 · Cited by 693
Phytochelatin Synthase Genes from Arabidopsis and the Yeast Schizosaccharomyces pombe
Ha, S.-B., Smith, A. P., Howden, R., Dietrich, W. M., Bugg, S., O'Connell, M. J., Goldsbrough, P. B., & Cobbett, C. S. (1999) · The Plant Cell · doi:10.1105/tpc.11.6.1153 · Cited by 659
P ATHWAYS AND R EGULATION OF S ULFUR M ETABOLISM R EVEALED T HROUGH M OLECULAR AND G ENETIC S TUDIES
Leustek, T., Martin, M. N., Bick, J.-A., & Davies, J. P. (2000) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.51.1.141 · Cited by 653
Vacuolar transporters and their essential role in plant metabolism
Martinoia, E., Maeshima, M., & Neuhaus, H. E. (2006) · Journal of Experimental Botany · doi:10.1093/jxb/erl183 · Cited by 621
Cadmium Tolerance and Accumulation in Indian Mustard Is Enhanced by Overexpressing γ-Glutamylcysteine Synthetase
Zhu, Y. L., Pilon‐Smits, E. A. H., Tarun, A. S., Weber, S. U., Jouanin, L., & Terry, N. (1999) · PLANT PHYSIOLOGY · doi:10.1104/pp.121.4.1169 · Cited by 577
Detoxification of Arsenic by Phytochelatins in Plants
Schmöger, M. E. V., Oven, M., & Grill, E. (2000) · PLANT PHYSIOLOGY · doi:10.1104/pp.122.3.793 · Cited by 574
Molecular mechanisms of heavy metal hyperaccumulation and phytoremediation
Yang, X., Feng, Y., He, Z. L., & Stoffella, P. J. (2005) · Journal of Trace Elements in Medicine and Biology · doi:10.1016/j.jtemb.2005.02.007 · Cited by 547
Phytoremediation: novel approaches to cleaning up polluted soils
Krämer, U. (2005) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2005.02.006 · Cited by 512
Regulatory networks of cadmium stress in plants
DalCorso, G., Farinati, S., & Furini, A. (2010) · Plant Signaling & Behavior · doi:10.4161/psb.5.6.11425 · Cited by 470
Heavy metal detoxification in eukaryotic microalgae
Perales-Vela, H. V., Peña-Castro, J. M., & Cañizares–Villanueva, R. O. (2006) · Chemosphere · doi:10.1016/j.chemosphere.2005.11.024 · Cited by 479
Changes in Gene Expression in Arabidopsis Shoots during Phosphate Starvation and the Potential for Developing Smart Plants
Hammond, J. P., Bennett, M. J., Bowen, H. C., Broadley, M. R., Eastwood, D. C., May, S. T., Rahn, C. R., Swarup, R., Woolaway, K. E., & White, P. J. (2003) · PLANT PHYSIOLOGY · doi:10.1104/pp.103.020941 · Cited by 454
Comparative microarray analysis of Arabidopsis thaliana and Arabidopsis halleri roots identifies nicotianamine synthase, a ZIP transporter and other genes as potential metal hyperaccumulation factors
Weber, M., Harada, E., Vess, C., Roepenack‐Lahaye, E. V., & Clemens, S. (2004) · The Plant Journal · doi:10.1046/j.1365-313x.2003.01960.x · Cited by 440
Transgenic Plants in Phytoremediation: Recent Advances and New Possibilities
Cherian, S., & Oliveira, M. M. (2005) · Environmental Science & Technology · doi:10.1021/es051134l · Cited by 427
Engineering tolerance and accumulation of lead and cadmium in transgenic plants
Song, W., Sohn, E. J., Martinoia, E., Lee, Y.-J., Yang, Y., Jasiński, M., Forestier, C., Hwang, I., & Lee, Y. (2003) · Nature Biotechnology · doi:10.1038/nbt850 · Cited by 420
Phytochelatin biosynthesis and function in heavy-metal detoxification
Cobbett, C. S. (2000) · Current Opinion in Plant Biology · doi:10.1016/s1369-5266(00)80067-9 · Cited by 418
Cadmium-inducible expression of the ABC-type transporter AtABCC3 increases phytochelatin-mediated cadmium tolerance in Arabidopsis
Brunetti, P., Zanella, L., Paolis, A. D., Litta, D. D., Cecchetti, V., Falasca, G., Barbieri, M., Altamura, M. M., Costantino, P., & Cardarelli, M. (2015) · Journal of Experimental Botany · doi:10.1093/jxb/erv185 · Cited by 408
Implications of metal accumulation mechanisms to phytoremediation
Memon, A. R., & Schröder, P. (2008) · Environmental Science and Pollution Research · doi:10.1007/s11356-008-0079-z · Cited by 399
Mechanism of Heavy Metal Ion Activation of Phytochelatin (PC) Synthase
Vatamaniuk, O. K., Mari, S., Lu, Y.-P., & Rea, P. A. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m002997200 · Cited by 390
Self-citation
The Biological Functions of Glutathione Revisited in Arabidopsis Transgenic Plants with Altered Glutathione Levels
Xiang, C., Werner, B. L., Christensen, E. M., & Oliver, D. J. (2001) · PLANT PHYSIOLOGY · doi:10.1104/pp.126.2.564 · Cited by 381
The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses
Sarry, J., Kühn, L., Ducruix, C., Lafaye, A., Junot, C., Hugouvieux, V., Jourdain, A., Bastien, O., Fiévet, J., Vailhen, D., et al. (2006) · PROTEOMICS · doi:10.1002/pmic.200500543 · Cited by 371
Heavy Metal Phytoremediation from Aquatic Ecosystems with Special Reference to Macrophytes
Rai, P. K. (2009) · Critical Reviews in Environmental Science and Technology · doi:10.1080/10643380801910058 · Cited by 374
Role of Hyperaccumulators in Phytoextraction of Metals From Contaminated Mining Sites: A Review
Sheoran, V., Sheoran, A. S., & Poonia, P. (2010) · Critical Reviews in Environmental Science and Technology · doi:10.1080/10643380902718418 · Cited by 350
Transport and detoxification of manganese and copper in plants
Dučić, T., & Polle, A. (2005) · Brazilian Journal of Plant Physiology · doi:10.1590/s1677-04202005000100009 · Cited by 348
Overexpression of Arabidopsis Phytochelatin Synthase Paradoxically Leads to Hypersensitivity to Cadmium Stress
Lee, S., Moon, J. S., Ko, T.-S., Petros, D., Goldsbrough, P. B., & Korban, S. S. (2003) · PLANT PHYSIOLOGY · doi:10.1104/pp.014118 · Cited by 345
Identification of high levels of phytochelatins, glutathione and cadmium in the phloem sap of Brassica napus . A role for thiol‐peptides in the long‐distance transport of cadmium and the effect of cadmium on iron translocation
Mendoza‐Cózatl, D. G., Butko, E., Springer, F., Torpey, J. W., Komives, E. A., Kehr, J., & Schroeder, J. I. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03410.x · Cited by 344
Mechanisms of Cadmium Accumulation in Plants
Sterckeman, T., & Thomine, S. (2020) · Critical Reviews in Plant Sciences · doi:10.1080/07352689.2020.1792179 · Cited by 343
Long-distance root-to-shoot transport of phytochelatins and cadmium in Arabidopsis
Gong, J., Lee, D. A., & Schroeder, J. I. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1734072100 · Cited by 338
Evolution and function of phytochelatin synthases
Clemens, S. (2005) · Journal of Plant Physiology · doi:10.1016/j.jplph.2005.11.010 · Cited by 327
Phytochelatins Are Involved in Differential Arsenate Tolerance inHolcus lanatus
Whitaker, J., Ainsworth, G. B., Vooijs, R., Bookum, W. M. T., Schat, H., & Meharg, A. A. (2001) · PLANT PHYSIOLOGY · doi:10.1104/pp.126.1.299 · Cited by 319
Transporters in plant sulfur metabolism
Gigolashvili, T., & Kopřiva, S. (2014) · Frontiers in Plant Science · doi:10.3389/fpls.2014.00442 · Cited by 313
Overexpressing GSH1 and AsPCS1 simultaneously increases the tolerance and accumulation of cadmium and arsenic in Arabidopsis thaliana
Guo, J., Dai, X., Xu, W., & Ma, M. (2008) · Chemosphere · doi:10.1016/j.chemosphere.2008.04.018 · Cited by 312
Phytoremediation of Heavy Metals: Physiological and Molecular Mechanisms
Jabeen, R., Ahmad, A., & Iqbal, M. M. (2009) · The Botanical Review · doi:10.1007/s12229-009-9036-x · Cited by 305
Phytoremediation of Metals Using Transgenic Plants
Pilon‐Smits, E. A. H., & Pilon, M. (2002) · Critical Reviews in Plant Sciences · doi:10.1080/0735-260291044313 · Cited by 293
Overexpression of Phytochelatin Synthase in Arabidopsis Leads to Enhanced Arsenic Tolerance and Cadmium Hypersensitivity
Li, Y., Dhankher, O. P., Carreira, L. H., Lee, D., Chen, A. P., Schroeder, J. I., Balish, R. S., & Meagher, R. B. (2004) · Plant and Cell Physiology · doi:10.1093/pcp/pch202 · Cited by 291
Accumulation and detoxification of lead ions in legumes
Piechalak, A., Tomaszewska, B., Barałkiewicz, D., & Małecka, A. (2002) · Phytochemistry · doi:10.1016/s0031-9422(02)00067-5 · Cited by 278
Plant Responses to Heavy Metal Toxicity
Manara, A. (2012) · Springer briefs in molecular science · doi:10.1007/978-94-007-4441-7_2 · Cited by 275
Bioavailability of trace metals to aquatic microorganisms: importance of chemical, biological and physical processes on biouptake
Worms, I. A. M., Simon, D. F., Hassler, C. S., & Wilkinson, K. J. (2006) · Biochimie · doi:10.1016/j.biochi.2006.09.008 · Cited by 253
The Ascorbate-glutathione-α-tocopherol Triad in Abiotic Stress Response
Szarka, A., Tomasskovics, B., & Bánhegyi, G. (2012) · International Journal of Molecular Sciences · doi:10.3390/ijms13044458 · Cited by 248
Phytochelatins: Peptides Involved in Heavy Metal Detoxification
Pal, R., & N., J. P. (2009) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-009-8565-4 · Cited by 245
Phytochelatin Synthesis Is Essential for the Detoxification of Excess Zinc and Contributes Significantly to the Accumulation of Zinc
Tennstedt, P., Peisker, D., Böttcher, C., Trampczynska, A., & Clemens, S. (2008) · PLANT PHYSIOLOGY · doi:10.1104/pp.108.127472 · Cited by 241
The use of transgenic plants in the bioremediation of soils contaminated with trace elements
Krämer, U., & Chardonnens, A. N. (2001) · Applied Microbiology and Biotechnology · doi:10.1007/s002530100631 · Cited by 245
Metal hyperaccumulation and bioremediation
Shah, K., & Nongkynrih, J. M. (2007) · Biologia Plantarum · doi:10.1007/s10535-007-0134-5 · Cited by 223
A New Pathway for Heavy Metal Detoxification in Animals
Vatamaniuk, O. K., Bucher, E. A., Ward, J. T., & Rea, P. A. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.c100152200 · Cited by 227
Self-citation
Transgenic Indian mustard (Brassica juncea) plants expressing an Arabidopsis phytochelatin synthase (AtPCS1) exhibit enhanced As and Cd tolerance
Gašić, K., & Korban, S. S. (2007) · Plant Molecular Biology · doi:10.1007/s11103-007-9158-7 · Cited by 220
Impacts of fly-ash on soil and plant responses
Gupta, D. K., Rai, U., Tripathi, R. D., & Inouhe, M. (2002) · Journal of Plant Research · doi:10.1007/s10265-002-0057-3 · Cited by 215
Heavy Metal Accumulation and Detoxification Mechanisms in Plants
Memon, A. R., Aktoprakligil, D., Özdemir, A., & Vertii, A. (2001) · DergiPark (Istanbul University) · Cited by 213
Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins
Bae, W., Chen, W., Mulchandani, A., & Mehra, R. K. (2000) · Biotechnology and Bioengineering · doi:10.1002/1097-0290(20001205)70:5 · Cited by 210
Phytochelatin synthase (PCS) protein is induced in Brassica juncea leaves after prolonged Cd exposure
Heiss, S. (2003) · Journal of Experimental Botany · doi:10.1093/jxb/erg205 · Cited by 195
Subcellular localization of cadmium in roots and leaves of Arabidopsis thaliana
Belleghem, F. V., Cuypers, A., Semane, B., Smeets, K., Vangronsveld, J., D’Haen, J., & Valcke, R. (2006) · New Phytologist · doi:10.1111/j.1469-8137.2006.01940.x · Cited by 200
Plant responses to environmental stresses—from gene to biotechnology
Ahanger, M. A., Akram, N. A., Ashraf, M., Alyemeni, M. N., Wijaya, L., & Ahmad, P. (2017) · AoB Plants · doi:10.1093/aobpla/plx025 · Cited by 196
Increased Nicotianamine Biosynthesis Confers Enhanced Tolerance of High Levels of Metals, in Particular Nickel, to Plants
Kim, S., Takahashi, M., Higuchi, K., Tsunoda, K., Nakanishi, H., Yoshimura, E., Mori, S., & Nishizawa, N. K. (2005) · Plant and Cell Physiology · doi:10.1093/pcp/pci196 · Cited by 185
The mechanistic basis of interactions between mycorrhizal associations and toxic metal cations
Meharg, A. A. (2003) · Mycological Research · doi:10.1017/s0953756203008608 · Cited by 185
Redox homeostasis in plants under abiotic stress: role of electron carriers, energy metabolism mediators and proteinaceous thiols
Kapoor, D., Sharma, R., Handa, N., Kaur, H., Rattan, A., Yadav, P. T., Gautam, V., Kaur, R., & Bhardwaj, R. (2015) · Frontiers in Environmental Science · doi:10.3389/fenvs.2015.00013 · Cited by 181
Phytochelatin–metal(loid) transport into vacuoles shows different substrate preferences in barley and A rabidopsis
Song, W., Mendoza‐Cózatl, D. G., Lee, Y., Schroeder, J. I., Ahn, S.-N., Lee, H., Wicker, T., & Martinoia, E. (2013) · Plant Cell & Environment · doi:10.1111/pce.12227 · Cited by 180
Phytochelatin and cadmium accumulation in wheat
Stolt, J. P., Sneller, F. E. C., Bryngelsson, T., Lundborg, T., & Schat, H. (2002) · Environmental and Experimental Botany · doi:10.1016/s0098-8472(02)00045-x · Cited by 180
Microbe and plant assisted-remediation of organic xenobiotics and its enhancement by genetically modified organisms and recombinant technology: A review
Hussain, I., Aleti, G., Naidu, R., Puschenreiter, M., Mahmood, Q., Rahman‬, M. M., Wang, F., Shaheen, S., Syed, J. H., & Reichenauer, T. G. (2018) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2018.02.037 · Cited by 179
Heavy Metal Stress in Plants
Gupta, D. K., Corpas, F. J., & Palma, J. M. (2013) · Springer eBooks · doi:10.1007/978-3-642-38469-1 · Cited by 176
Biosynthetic regulation of phytochelatins, heavy metal-binding peptides
Hirata, K., Tsuji, N., & Miyamoto, K. (2005) · Journal of Bioscience and Bioengineering · doi:10.1263/jbb.100.593 · Cited by 164
Differential response of arsenic stress in two varieties of Brassica juncea L.
Gupta, M., Sharma, P., Sarin, N. B., & Sinha, A. K. (2008) · Chemosphere · doi:10.1016/j.chemosphere.2008.11.023 · Cited by 164
Phytochelatins: Sulfur-Containing Metal(loid)-Chelating Ligands in Plants
Seregin, I. V., & Kozhevnikova, A. D. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24032430 · Cited by 165
Enhanced Toxic Metal Accumulation in Engineered Bacterial Cells Expressing Arabidopsis thaliana Phytochelatin Synthase
Sauge‐Merle, S., Cuiné, S., Carrier, P., Lecomte-Pradines, C., Luu, D., & Peltier, G. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.1.490-494.2003 · Cited by 159
Fortified Foods and Phytoremediation. Two Sides of the Same Coin
Guerinot, M. L., & Salt, D. E. (2001) · PLANT PHYSIOLOGY · doi:10.1104/pp.125.1.164 · Cited by 154
Functions and homeostasis of zinc, copper, and nickel in plants
Krämer, U., & Clemens, S. (2005) · Topics in current genetics · doi:10.1007/4735_96 · Cited by 157
Phytoremediation of toxic metals from soil and waste water.
Hooda, V. (2007) · PubMed · Cited by 152
Cadmium tolerance and phytochelatin content of Arabidopsis seedlings over-expressing the phytochelatin synthase gene AtPCS1
Brunetti, P., Zanella, L., Proia, A., Paolis, A. D., Falasca, G., Altamura, M. M., di Toppi, L. S., Costantino, P., & Cardarelli, M. (2011) · Journal of Experimental Botany · doi:10.1093/jxb/err228 · Cited by 150
A Review on Mechanisms of Plant Tolerance and Role of Transgenic Plants in Environmental Clean-up
Seth, C. S. (2011) · The Botanical Review · doi:10.1007/s12229-011-9092-x · Cited by 149
Arabidopsis metallothioneins 2a and 3 enhance resistance to cadmium when expressed in Vicia faba guard cells
Lee, J., Donghwan, S., Song, W., Inhwan, H., & Youngsook, L. (2004) · Plant Molecular Biology · doi:10.1007/s11103-004-0190-6 · Cited by 147
An Improved Grafting Technique for Mature Arabidopsis Plants Demonstrates Long-Distance Shoot-to-Root Transport of Phytochelatins in Arabidopsis
Chen, A. P., Komives, E. A., & Schroeder, J. I. (2006) · PLANT PHYSIOLOGY · doi:10.1104/pp.105.072637 · Cited by 146
Function of phytochelatin synthase in catabolism of glutathione‐conjugates
Blum, R., Beck, A., Korte, A., Stengel, A., Letzel, T., Lendzian, K. J., & Grill, E. (2007) · The Plant Journal · doi:10.1111/j.1365-313x.2006.02993.x · Cited by 143
Caenorhabditis elegans expresses a functional phytochelatin synthase
Clemens, S., Schroeder, J. I., & Degenkolb, T. (2001) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.2001.02293.x · Cited by 143
miR395 is involved in detoxification of cadmium in Brassica napus
Zhang, L. W., Song, J. B., Shu, X., Zhang, Y., & Yang, Z. M. (2013) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2013.01.053 · Cited by 139
The Shoot-Specific Expression of γ-Glutamylcysteine Synthetase Directs the Long-Distance Transport of Thiol-Peptides to Roots Conferring Tolerance to Mercury and Arsenic
Li, Y., Dankher, O. P., Carreira, L. H., Smith, A. P., & Meagher, R. B. (2006) · PLANT PHYSIOLOGY · doi:10.1104/pp.105.074815 · Cited by 139
HEAVY METAL STRESS
Gašić, K., & Korban, S. S. (2006) · Springer eBooks · doi:10.1007/1-4020-4225-6_8 · Cited by 138
Phytochelatin Synthase, a Dipeptidyltransferase That Undergoes Multisite Acylation with γ-Glutamylcysteine during Catalysis
Vatamaniuk, O. K., Mari, S., Lang, A., Chalasani, S. H., Demkiv, L. O., & Rea, P. A. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m313142200 · Cited by 133
Self-citation
Overexpression of phytochelatin synthase in tobacco: distinctive effects of AtPCS1 and CePCS genes on plant response to cadmium
Wojas, S., Clemens, S., Hennig, J., Skłodowska, A., Kopera, E., Schat, H., Bal, W., & Antosiewicz, D. M. (2008) · Journal of Experimental Botany · doi:10.1093/jxb/ern092 · Cited by 133
Rice phytochelatin synthases Os PCS 1 and Os PCS 2 make different contributions to cadmium and arsenic tolerance
Yamazaki, S., Ueda, Y., Mukai, A., Ochiai, K., & Matoh, T. (2018) · Plant Direct · doi:10.1002/pld3.34 · Cited by 131
Critical Review of the Scienceand Options for Reducing Cadmium in Tobacco (Nicotiana Tabacum L. ) and Other Plants
Lugon‐Moulin, N., Zhang, M., Gadani, F., Rossi, L., Koller, D. C., Krauss, M. R., & Wagner, G. J. (2004) · Advances in agronomy · doi:10.1016/s0065-2113(04)83003-7 · Cited by 131
Reactive oxygen and nitrogen species as key indicators of plant responses to Cd stress
Romero‐Puertas, M. C., Terron-Camero, L. C., Peláez‐Vico, M. Á., Olmedilla, A., & Sandalio, L. M. (2018) · Environmental and Experimental Botany · doi:10.1016/j.envexpbot.2018.10.012 · Cited by 130
Modeling Cadmium Uptake and Accumulation in Plants
Tudoreanu, L., & Phillips, C. J. C. (2004) · Advances in agronomy · doi:10.1016/s0065-2113(04)84003-3 · Cited by 126
Phytochelatins: Key regulator against heavy metal toxicity in plants
Faizan, M., Alam, P., Hussain, A., Karabulut, F., Tonny, S. H., Cheng, S. H., Yusuf, M., Adil, M. F., Sehar, S., Alomrani, S. O., et al. (2024) · Plant Stress · doi:10.1016/j.stress.2024.100355 · Cited by 126
Evolution of Abscisic Acid Signaling for Stress Responses to Toxic Metals and Metalloids
Hu, B., Deng, F., Chen, G. J., Chen, X., Gao, W., Long, L., Xia, J., & Chen, Z. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00909 · Cited by 125
Weeds, Worms, and More. Papain's Long-Lost Cousin, Phytochelatin Synthase
Rea, P. A., Vatamaniuk, O. K., & Rigden, D. J. (2004) · PLANT PHYSIOLOGY · doi:10.1104/pp.104.048579 · Cited by 125
Self-citation
Expression of Arabidopsis phytochelatin synthase in Indian mustard (Brassica juncea) plants enhances tolerance for Cd and Zn
Gašić, K., & Korban, S. S. (2006) · Planta · doi:10.1007/s00425-006-0421-y · Cited by 126
Production of Inorganic Nanoparticles by Microorganisms
Krumov, N., Perner-Nochta, I., Oder, S., Gotcheva, V., Angelov, A., & Posten, C. H. (2009) · Chemical Engineering & Technology · doi:10.1002/ceat.200900046 · Cited by 122
Identification of alternatively spliced transcripts of rice phytochelatin synthase 2 gene OsPCS2 involved in mitigation of cadmium and arsenic stresses
Das, N., Bhattacharya, S., Bhattacharyya, S., & Maiti, M. K. (2017) · Plant Molecular Biology · doi:10.1007/s11103-017-0600-1 · Cited by 122
Transgenic tobacco plants expressing a rice cysteine synthase gene are tolerant to toxic levels of cadmium
Harada, E., Choi, Y.-E., Tsuchisaka, A., Obata, H., & Sano, H. (2001) · Journal of Plant Physiology · doi:10.1078/0176-1617-00314 · Cited by 122
Nicotianamine Over-accumulation Confers Resistance to Nickel in Arabidopsis thaliana
Pianelli, K., Mari, S., Marqués, L. P., Lebrun, M., & Czernic, P. (2005) · Transgenic Research · doi:10.1007/s11248-005-7159-3 · Cited by 125
Self-citation
Molecular Characterization of the Homo-phytochelatin Synthase of Soybean Glycine max
Oven, M., Page, J. E., Zenk, M. H., & Kutchan, T. M. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m108254200 · Cited by 119
Phytochelatins
Inouhe, M. (2005) · Brazilian Journal of Plant Physiology · doi:10.1590/s1677-04202005000100006 · Cited by 123

Authors: Vatamaniuk, O. K., Mari, S., Lu, Y.-P., & Rea, P. A.
Affiliations: University of Pennsylvania (US)
ORCID: Vatamaniuk, Olena K.
Mari, Stéphane
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1999-06-08
Volume: 96
Issue: 12
Pages: 7110-7115
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.96.12.7110
PMC: PMC22073
OpenAlex: W2096818569
Cited by: 410
References: 34
Citations by year: 2026: 6 · 2025: 4 · 2024: 6 · 2023: 8 · 2022: 13 · 2021: 9 · 2020: 11 · 2019: 11 · 2018: 11 · 2017: 8 · 2016: 10 · 2015: 22 · 2014: 8 · 2013: 12 · 2012: 19
FWCI: 29.02
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
Keywords: phytochelatin synthase, phytochelatins, cadmium tolerance, heavy metal tolerance, glutathione, Arabidopsis thaliana, Saccharomyces cerevisiae expression, metal chelation
MeSH terms: Amino Acid Sequence, Molecular Sequence Data, Plant Proteins, Saccharomyces cerevisiae, Sequence Alignment, Arabidopsis, Aminoacyltransferases

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

AtPCS1, a phytochelatin synthase from Arabidopsis : Isolation and in vitro reconstitution

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks