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Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activity
Open accessAbstract
Because hematopoietic stem cells are rich in aldehyde dehydrogenase (ALDH) activity, we developed a fluorescent substrate for ALDH, termed BODIPY aminoacetaldehyde (BAAA), and tested its potential for isolating primitive human hematopoietic cells. A population of cells with low orthogonal light scattering and bright fluorescence intensity (SSC lo ALDH br cells) could be readily fractionated from human umbilical cord blood cells costained with BAAA and the multidrug-resistance inhibitor verapamil. The SSC lo ALDH br population was depleted of lineage-committed cells, 40–90% pure for CD34+CD38 lo/−cells, and enriched 50- to 100-fold for primitive hematopoietic progenitors detected in short- and long-term culture analyses. Together, these observations indicate that fractionating human hematopoietic stem cells on the basis of ALDH activity using BAAA is an effective method for isolating primitive human hematopoietic progenitors. This technique may be useful for isolating stem cells from other tissues as well.
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Cited by
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van den Hoogen, C., van der Horst, G., Cheung, H., Buijs, J. T., Lippitt, J. M., Guzmán-Ramírez, N., Hamdy, F. C., Eaton, C. L., Thalmann, G. N., Cecchini, M. G., et al. (2010) · Cancer Research · doi:10.1158/0008-5472.can-09-3806 · Cited by 398
Kanakry, C. G., Ganguly, S., Zahurak, M., Bolaños-Meade, J., Thoburn, C., Perkins, B., Fuchs, E. J., Jones, R. J., Hess, A. D., & Luznik, L. (2013) · Science Translational Medicine · doi:10.1126/scitranslmed.3006960 · Cited by 357
Metadata
- Authors
- Storms, R. W., Trujillo, A. P., Springer, J. B., Shah, L., Colvin, O. M., Ludeman, S. M., & Smith, C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-08-03
- Volume
- 96
- Issue
- 16
- Pages
- 9118-9123
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2014406829
- Cited by
- 551
- References
- 0
- FWCI
- 1.66
- Type
- journal article
- Language
- en
- Open access
- green
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- Storms, R. W., Trujillo, A. P., Springer, J. B., Shah, L., Colvin, O. M., Ludeman, S. M., & Smith, C. (1999). Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activity. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.16.9118
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