The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Molecular characterization of a slowly gating human hyperpolarization-activated channel predominantly expressed in thalamus, heart, and testis
Open accessAbstract
Rhythmic activity of neurons and heart cells is endowed by pacemaker channels that are activated by hyperpolarization and directly regulated by cyclic nucleotides (termed HCN channels). These channels constitute a multigene family, and it is assumed that the properties of each member are adjusted to fit its particular function in the cell in which it resides. Here we report the molecular and functional characterization of a human subtype hHCN4. hHCN4 transcripts are expressed in heart, brain, and testis. Within the brain, the thalamus is the predominant area of hHCN4 expression. Heterologous expression of hHCN4 produces channels of unusually slow kinetics of activation and inactivation. The mean potential of half-maximal activation (V 1/2 ) was −75.2 mV. cAMP shifted V 1/2 by 11 mV to more positive values. The hHCN4 gene was mapped to chromosome band 15q24–q25. The characteristic expression pattern and the sluggish gating suggest that hHCN4 controls the rhythmic activity in both thalamocortical neurons and pacemaker cells of the heart.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Thalamocortical oscillations in the sleeping and aroused brain
Steriade, M., McCormick, D. A., & Sejnowski, T. J. (1993) · Science · doi:10.1126/science.8235588 · Cited by 3,564
High-Resolution Mapping of Human Chromosome 11 by in Situ Hybridization with Cosmid Clones
Lichter, P., Tang, C.-J. C., Call, K., Hermanson, G., Evans, G. A., Housman, D., & Ward, D. C. (1990) · Science · doi:10.1126/science.2294592 · Cited by 1,331
Self-citationQueer Current and Pacemaker: The Hyperpolarization-Activated Cation Current in Neurons
Pape, H.-C. (1996) · Annual Review of Physiology · doi:10.1146/annurev.ph.58.030196.001503 · Cited by 1,151
McCormick, D. A., & Pape, H. C. (1990) · The Journal of Physiology · doi:10.1113/jphysiol.1990.sp018331 · Cited by 1,139
Voltage-clamp analysis of muscarinic excitation in hippocampal neurons
Halliwell, J. V., & Adams, P. R. (1982) · Brain Research · doi:10.1016/0006-8993(82)90954-4 · Cited by 1,041
A family of hyperpolarization-activated mammalian cation channels
Ludwig, A., Zong, X., Jeglitsch, M., Hofmann, F., & Biel, M. (1998) · Nature · doi:10.1038/31255 · Cited by 943
Pacemaker Mechanisms in Cardiac Tissue
DiFrancesco, D. (1993) · Annual Review of Physiology · doi:10.1146/annurev.ph.55.030193.002323 · Cited by 862
Direct activation of cardiac pacemaker channels by intracellular cyclic AMP
DiFrancesco, D., & Tortora, P. (1991) · Nature · doi:10.1038/351145a0 · Cited by 850
Identification of a Gene Encoding a Hyperpolarization-Activated Pacemaker Channel of Brain
Santoro, B., Liu, D. T., Yao, H., Bartsch, D., Kandel, E. R., Siegelbaum, S. A., & Tibbs, G. R. (1998) · Cell · doi:10.1016/s0092-8674(00)81434-8 · Cited by 704
Molecular identification of a hyperpolarization-activated channel in sea urchin sperm
Gauss, R., Seifert, R., & Kaupp, U. B. (1998) · Nature · doi:10.1038/31248 · Cited by 469
Self-citation
Cited by
Santoro, B., Chen, S., Lüthi, A., Pavlidis, P., Shumyatsky, G. P., Tibbs, G. R., & Siegelbaum, S. A. (2000) · The Journal of Neuroscience · doi:10.1523/jneurosci.20-14-05264.2000 · Cited by 600
Genesis and Regulation of the Heart Automaticity
Mangoni, M. E., & Nargeot, J. (2008) · Physiological Reviews · doi:10.1152/physrev.00018.2007 · Cited by 600
The Role of the Funny Current in Pacemaker Activity
DiFrancesco, D. (2010) · Circulation Research · doi:10.1161/circresaha.109.208041 · Cited by 587
Immunohistochemical localization of Ih channel subunits, HCN1–4, in the rat brain
Notomi, T., & Shigemoto, R. (2004) · Journal of Comparative Neurology · doi:10.1002/cne.11039 · Cited by 558
Polarized and compartment-dependent distribution of HCN1 in pyramidal cell dendrites
Lörincz, A., Notomi, T., Tamás, G., Shigemoto, R., & Nusser, Z. (2002) · Nature Neuroscience · doi:10.1038/nn962 · Cited by 441
Metadata
- Authors
- Seifert, R., Scholten, A., Gauss, R., Mincheva, A., Lichter, P., & Kaupp, U. B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-08-03
- Volume
- 96
- Issue
- 16
- Pages
- 9391-9396
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2018527368
- Cited by
- 277
- References
- 34
- FWCI
- 4.78
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Molecular characterization of a slowly gating human hyperpolarization-activated channel predominantly expressed in thalamus, heart, and testis. https://app.cochat.ai/papers/10.1073/pnas.96.16.9391
- The paper
- Seifert, R., Scholten, A., Gauss, R., Mincheva, A., Lichter, P., & Kaupp, U. B. (1999). Molecular characterization of a slowly gating human hyperpolarization-activated channel predominantly expressed in thalamus, heart, and testis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.16.9391
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
