The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Evidence for a conserved system for iron metabolism in the mitochondria of Saccharomyces cerevisiae
Open accessAbstract
nifU of nitrogen-fixing bacteria is involved in the synthesis of the Fe–S cluster of nitrogenase. In a synthetic lethal screen with the mitochondrial heat shock protein (HSP)70, SSQ1 , we identified a gene of Saccharomyces cerevisiae , NFU1 , which encodes a protein with sequence identity to the C-terminal domain of NifU. Two other yeast genes were found to encode proteins related to the N-terminal domain of bacterial NifU. They have been designated ISU1 and ISU2 . Isu1, Isu2, and Nfu1 are located in the mitochondrial matrix. ISU genes of yeast carry out an essential function, because a Δisu1Δisu2 strain is inviable. Growth of Δnfu1Δ isu1 cells is significantly compromised, allowing assessment of the physiological roles of Nfu and Isu proteins. Mitochondria from Δnfu1Δisu1 cells have decreased activity of several respiratory enzymes that contain Fe–S clusters. As a result, Δnfu1Δisu1 cells grow poorly on carbon sources requiring respiration. Δnfu1Δisu1 cells also accumulate abnormally high levels of iron in their mitochondria, similar to Δssq1 cells, indicating a role for these proteins in iron metabolism. We suggest that NFU1 and ISU1 gene products play a role in iron homeostasis, perhaps in assembly, insertion, and/or repair of mitochondrial Fe–S clusters. The conservation of these protein domains in many organisms suggests that this role has been conserved throughout evolution.
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Grx5 Is a Mitochondrial Glutaredoxin Required for the Activity of Iron/Sulfur Enzymes
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Metadata
- Authors
- Schilke, B., Voisine, C., Beinert, H., & Craig, E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-08-31
- Volume
- 96
- Issue
- 18
- Pages
- 10206-10211
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2153371171
- Cited by
- 284
- References
- 33
- FWCI
- 23.60
- Type
- journal article
- Language
- en
- Open access
- green
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