The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants
Open accessAbstract
Rapid production of protein-based tumor-specific vaccines for the treatment of malignancies is possible with the plant-based transient expression system described here. We created a modified tobamoviral vector that encodes the idiotype-specific single-chain Fv fragment (scFv) of the immunoglobulin from the 38C13 mouse B cell lymphoma. Infected Nicotiana benthamiana plants contain high levels of secreted scFv protein in the extracellular compartment. This material reacts with an anti-idiotype antibody by Western blotting, ELISA, and affinity chromatography, suggesting that the plant-produced 38C13 scFv protein is properly folded in solution. Mice vaccinated with the affinity-purified 38C13 scFv generate >10 μg/ml anti-idiotype immunoglobulins. These mice were protected from challenge by a lethal dose of the syngeneic 38C13 tumor, similar to mice immunized with the native 38C13 IgM-keyhole limpet hemocyanin conjugate vaccine. This rapid production system for generating tumor-specific protein vaccines may provide a viable strategy for the treatment of non-Hodgkin’s lymphoma.
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Cited by
Protein Therapeutics: A Summary and Pharmacological Classification
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The production of recombinant pharmaceutical proteins in plants
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Magnifection?a new platform for expressing recombinant vaccines in plants
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Metadata
- Authors
- McCormick, A. A., Kumagai, M. H., Hanley, K., Turpen, T. H., Hakim, I., Grill, L. K., Tusé, D., Levy, S., & Levy, R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-01-19
- Volume
- 96
- Issue
- 2
- Pages
- 703-708
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1985374701
- Cited by
- 275
- References
- 29
- FWCI
- 25.26
- Type
- journal article
- Language
- en
- Open access
- green
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- McCormick, A. A., Kumagai, M. H., Hanley, K., Turpen, T. H., Hakim, I., Grill, L. K., Tusé, D., Levy, S., & Levy, R. (1999). Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.2.703
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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