The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Abscisic acid signal transduction in guard cells is mediated by phospholipase D activity
Open accessAbstract
In guard cells, the plant hormone abscisic acid (ABA) inhibits stomatal opening and induces stomatal closure through the coordinated regulation of ion transport. Despite this central role of ABA in regulating stomatal function, the signal transduction events leading to altered ion fluxes remain incompletely understood. We report that the activity of the enzyme phospholipase D (PLD) transiently increased in guard cell protoplasts at 2.5 and 25 min after ABA application. Treatment of guard cell protoplasts with phosphatidic acid (PtdOH), one of the products of PLD activity, led to an inhibition of the activity of the inward K + channel. PtdOH also induced stomatal closure and inhibited stomatal opening when added to epidermal peels. Application of 1-butanol (1-buOH), a selective inhibitor of PtdOH production by PLD, inhibited the increase in PtdOH production elicited by ABA. 1-BuOH treatment also partially prevented ABA-induced stomatal closure and ABA-induced inhibition of stomatal opening. This inhibitory effect of buOH was enhanced by simultaneous application of nicotinamide, an inhibitor of cADP ribose action. These results suggest that in the guard cell, ABA activates the enzyme PLD, which leads to the production of PtdOH. This PtdOH is then involved in triggering subsequent ABA responses of the cell via a pathway operating in parallel to cADP ribose-mediated events.
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Cites
Arabidopsis ABA Response Gene ABI1 : Features of a Calcium-Modulated Protein Phosphatase
Leung, J., Bouvier-Durand, M., Morris, P.-C., Guerrier, D., Chefdor, F., & Giraudat, J. (1994) · Science · doi:10.1126/science.7910981 · Cited by 767
Bleasdale, J. E., Thakur, N. R., Gremban, R. S., Bundy, G. L., Fitzpatrick, F. A., Smith, R. J., & Bunting, S. (1990) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)22967-6 · Cited by 627
Abscisic acid-induced elevation of guard cell cytosolic Ca2+ precedes stomatal closure
McAinsh, M. R., Brownlee, C., & Hetherington, A. M. (1990) · Nature · doi:10.1038/343186a0 · Cited by 535
Pei, Z. M., Kuchitsu, K., Ward, J. M., Schwarz, M., & Schroeder, J. I. (1997) · The Plant Cell · doi:10.1105/tpc.9.3.409 · Cited by 450
Phospholipid signalling in plants
Munnik, T., Irvine, R. F., & Musgrave, A. (1998) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/s0005-2760(97)00158-6 · Cited by 421
Signal Transduction in Guard Cells
Assmann, S. M. (1993) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.09.110193.002021 · Cited by 411
Self-citationRole of Farnesyltransferase in ABA Regulation of Guard Cell Anion Channels and Plant Water Loss
Pei, Z.-M., Ghassemian, M., Kwak, C. M., McCourt, P., & Schroeder, J. I. (1998) · Science · doi:10.1126/science.282.5387.287 · Cited by 390
Staxén, I., Pical, C., Montgomery, L. T., Gray, J. E., Hetherington, A. M., & McAinsh, M. R. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.4.1779 · Cited by 363
Schroeder, J. I., & Hagiwara, S. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.23.9305 · Cited by 359
Role of Calcium in Signal Transduction of Commelina Guard Cells.
Gilroy, S., Fricker, M. D., Read, N. D., & Trewavas, A. J. (1991) · The Plant Cell · doi:10.1105/tpc.3.4.333 · Cited by 342
Self-citation
Cited by
Cell Signaling during Cold, Drought, and Salt Stress
Xiong, L., Schumaker, K. S., & Zhu, J.-K. (2002) · The Plant Cell · doi:10.1105/tpc.000596 · Cited by 2,372
Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells
Pei, Z.-M., Murata, Y., Benning, G., Thomine, S., Klüsener, B., Allen, G. J., Grill, E., & Schroeder, J. I. (2000) · Nature · doi:10.1038/35021067 · Cited by 2,109
Signaling functions of phosphatidic acid
WANG, X., DEVAIAH, S., ZHANG, W., & WELTI, R. (2006) · Progress in Lipid Research · doi:10.1016/j.plipres.2006.01.005 · Cited by 757
Hydrogen Peroxide Is Involved in Abscisic Acid-Induced Stomatal Closure in Vicia faba
Zhang, X., Zhang, L., Dong, F., Gao, J., Galbraith, D. W., & Song, C.-P. (2001) · Plant Physiology · doi:10.1104/pp.126.4.1438 · Cited by 659
Phospholipid-Based Signaling in Plants
Meijer, H. J. G., & Munnik, T. (2003) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.54.031902.134748 · Cited by 587
Metadata
- Authors
- Jacob, T., Ritchie, S., Assmann, S. M., & Gilroy, S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-10-12
- Volume
- 96
- Issue
- 21
- Pages
- 12192-12197
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2017267000
- Cited by
- 288
- References
- 35
- FWCI
- 31.31
- Type
- journal article
- Language
- en
- Open access
- green
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