Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells
Abstract
Drought is a major threat to agricultural production. Plants synthesize the hormone abscisic acid (ABA) in response to drought, triggering a signalling cascade in guard cells that results in stomatal closure, thus reducing water loss. ABA triggers an increase in cytosolic calcium in guard cells ([Ca2+]cyt) that has been proposed to include Ca2+ influx across the plasma membrane. However, direct recordings of Ca2+ currents have been limited and the upstream activation mechanisms of plasma membrane Ca2+ channels remain unknown. Here we report activation of Ca2+-permeable channels in the plasma membrane of Arabidopsis guard cells by hydrogen peroxide. The H2O2-activated Ca2+ channels mediate both influx of Ca2+ in protoplasts and increases in [Ca2+]cyt in intact guard cells. ABA induces the production of H2O2 in guard cells. If H2O2 production is blocked, ABA-induced closure of stomata is inhibited. Moreover, activation of Ca2+ channels by H2O2 and ABA- and H2O2-induced stomatal closing are disrupted in the recessive ABA-insensitive mutant gca2. These data indicate that ABA-induced H2O2 production and the H2O2-activated Ca2+ channels are important mechanisms for ABA-induced stomatal closing.
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Accumulating ROS activates Ca2+-channels, allowing Ca2+ to enter the cytosol and increasing cytosolic free Ca2+ concentration.
“The accumulating ROS also activates Ca2+-channels, allowing Ca2+ to enter the cytosol, and increase in cytosolic free Ca2+ concentration ([Ca2+]cyt)”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Pei et al., 2000
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“The accumulating ROS also activates Ca2+-channels, allowing Ca2+ to enter the cytosol, and increase in cytosolic free Ca2+ concentration ([Ca]cyt) (Pei et al., 2000).”
Background
- Authors
- Pei, Z.-M., Murata, Y., Benning, G., Thomine, S., Klüsener, B., Allen, G. J., Grill, E., & Schroeder, J. I.
- Affiliations
- University of California San Diego (US)
- Technical University of Munich (DE)
- Corresponding author
- Schroeder, Julian I.
- Venue
- Nature
- Published
- 2000-08-17
- Volume
- 406
- Issue
- 6797
- Pages
- 731-734
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1902612187
- Cited by
- 2,109
- References
- 34
- Citations by year
- 2026: 49 · 2025: 53 · 2024: 82 · 2023: 74 · 2022: 93 · 2021: 88 · 2020: 103 · 2019: 79 · 2018: 88 · 2017: 96 · 2016: 84 · 2015: 100 · 2014: 85 · 2013: 107 · 2012: 85
- FWCI
- 94.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant and Biological Electrophysiology Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Keywords
- abscisic acid signaling, guard cells, hydrogen peroxide, calcium channels, stomatal closure, drought stress, intracellular calcium, Arabidopsis thaliana, reactive oxygen species
- MeSH terms
- Abscisic Acid, Hydrogen Peroxide, Mutation, Calcium Channels, Signal Transduction, Ion Channel Gating, Arabidopsis, Reactive Oxygen Species, Patch-Clamp Techniques
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