Plasmodium falciparum domain mediating adhesion to chondroitin sulfate A: A receptor for human placental infection
Open accessAbstract
Malaria during the first pregnancy causes a high rate of fetal and neonatal death. The decreasing susceptibility during subsequent pregnancies correlates with acquisition of antibodies that block binding of infected red cells to chondroitin sulfate A (CSA), a receptor for parasites in the placenta. Here we identify a domain within a particular Plasmodium falciparum erythrocyte membrane protein 1 that binds CSA. We cloned a var gene expressed in CSA-binding parasitized red blood cells (PRBCs). The gene had eight receptor-like domains, each of which was expressed on the surface of Chinese hamster ovary cells and was tested for CSA binding. CSA linked to biotin used as a probe demonstrated that two Duffy-binding-like (DBL) domains (DBL3 and DBL7) bound CSA. DBL7, but not DBL3, also bound chondroitin sulfate C (CSC) linked to biotin, a negatively charged sugar that does not support PRBC adhesion. Furthermore, CSA, but not CSC, blocked the interaction with DBL3; both CSA and CSC blocked binding to DBL7. Thus, only the DBL3 domain displays the same binding specificity as PRBCs. Because protective antibodies present after pregnancy block binding to CSA of parasites from different parts of the world, DBL-3, although variant, may induce cross-reactive immunity that will protect pregnant women and their fetuses.
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Several groups have identified CSA-binding domains in PfEMP1 molecules.
“Several groups have identified CSA-binding domains in PfEMP1 molecules (19-21).”
From Evidence for the Involvement of VAR2CSA in Pregnancy-associated Malaria (2004) · cites this paper as [19]
Cites
Su, X.-Z., Heatwole, V. M., Wertheimer, S. P., Guinet, F., Herrfeldt, J. A., Peterson, D. S., Ravetch, J. A., & Wellems, T. E. (1995) · Cell · doi:10.1016/0092-8674(95)90055-1 · Cited by 1,219 · see citing works
Adherence of <i>Plasmodium falciparum</i> to Chondroitin Sulfate A in the Human Placenta
Fried, M., & Duffy, P. E. (1996) · Science · doi:10.1126/science.272.5267.1502 · Cited by 1,108 · see citing works
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Salanti, A., Staalsoe, T., Lavstsen, T., Jensen, A. T. R., Sowa, M. P. K., Arnot, D. E., Hviid, L., & Theander, T. G. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03570.x · Cited by 702 · see citing works
Malaria in pregnancy: pathogenesis and immunity
Rogerson, S. J., Hviid, L., Duffy, P. E., Leke, R. F., & Taylor, D. W. (2007) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(07)70022-1 · Cited by 628 · see citing works
Evidence for the Involvement of VAR2CSA in Pregnancy-associated Malaria
Salanti, A., Dahlbäck, M., Turner, L., Nielsen, M. A., Barfod, L., Magistrado, P., Jensen, A. T. R., Lavstsen, T., Ofori, M. F., Marsh, K., et al. (2004) · The Journal of Experimental Medicine · doi:10.1084/jem.20041579 · Cited by 604 · see citing works
Ricke, C. H., Staalsoe, T., Koram, K., Akanmori, B. D., Riley, E. M., Theander, T. G., & Hviid, L. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.165.6.3309 · Cited by 303 · see citing works
Staalsoe, T., Shulman, C. E., Bulmer, J. N., Kawuondo, K., Marsh, K., & Hviid, L. (2004) · The Lancet · doi:10.1016/s0140-6736(03)15386-x · Cited by 288 · see citing works
Viebig, N. K., Gamain, B., Scheidig, C., Lépolard, C., Przyborski, J., Lanzer, M., Gysin, J., & Scherf, A. (2005) · The EMBO Reports · doi:10.1038/sj.embor.7400466 · Cited by 201 · see citing works
Self-citationHigh Level of<i>var2csa</i>Transcription by<i>Plasmodium falciparum</i>Isolated from the Placenta
Tuikue Ndam, N. G., Salanti, A., Bertin, G., Dahlbäck, M., Fievet, N., Turner, L., Gaye, A., Theander, T., & Deloron, P. (2005) · The Journal of Infectious Diseases · doi:10.1086/430933 · Cited by 179 · see citing works
Staalsoe, T., Megnekou, R., Fievét, N., Ricke, C. H., Zornig, H. D., Leke, R., Taylor, D. W., Deloron, P., & Hviid, L. (2001) · The Journal of Infectious Diseases · doi:10.1086/322809 · Cited by 161 · see citing works
Gamain, B., Trimnell, A. R., Scheidig, C., Scherf, A., Miller, L. H., & Smith, J. D. (2005) · The Journal of Infectious Diseases · doi:10.1086/428137 · Cited by 145 · see citing works
Self-citationConroy, A., Serghides, L., Finney, C., Owino, S. O., Kumar, S., Gowda, D. C., Liles, W. C., Moore, J. M., & Kain, K. C. (2009) · PLoS ONE · doi:10.1371/journal.pone.0004953 · Cited by 81 · see citing works
Hviid, L. (2010) · Human Vaccines · doi:10.4161/hv.6.1.9602 · Cited by 75 · see citing works
Plasmodium falciparum adhesion in the placenta
Duffy, P. E., & Fried, M. (2003) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(03)00090-0 · Cited by 73 · see citing works
Francis, S. E., Malkov, V. A., Oleinikov, A. V., Rossnagle, E., Wendler, J. P., Mutabingwa, T. K., Fried, M., & Duffy, P. E. (2007) · Infection and Immunity · doi:10.1128/iai.00635-07 · Cited by 65 · see citing works
Characterization of anti-var2CSA-PfEMP1 cytoadhesion inhibitory mouse monoclonal antibodies
Avril, M., Gamain, B., Lépolard, C., Viaud, N., Scherf, A., & Gysin, J. (2006) · Microbes and Infection · doi:10.1016/j.micinf.2006.09.005 · Cited by 61 · see citing works
Self-citationBir, N., Yazdani, S. S., Avril, M., Layez, C., Gysin, J., & Chitnis, C. E. (2006) · Infection and Immunity · doi:10.1128/iai.00481-06 · Cited by 49 · see citing works
Self-citation
- Authors
- Buffet, P. A., Gamain, B., Scheidig, C., Baruch, D., Smith, J. D., Hernandez-Rivas, R., Pouvelle, B., Oishi, S., Fujii, N., Fusai, T., Parzy, D., Miller, L. H., Gysin, J., & Scherf, A.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Unité de Biologie des Interactions Hôte-Parasite, Centre National de la Recherche Scientifique/Unité de Recherche Associée 1960, Institut Pasteur, 75724 Paris, France; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0425; CINVESTAV-IPN, Departamento de Biomedicina Molecular, C.P. 07000 Mexico City, Mexico; Unité de Parasitologie Expérimentale, Université de la Méditerranée, Aix–Marseille II, 13385, Marseille,...
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1999-10-26
- Volume
- 96
- Issue
- 22
- Pages
- 12743-12748
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC23079
- OpenAlex
- W2134191208
- Cited by
- 239
- References
- 2
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 2 · 2017: 4 · 2016: 7 · 2015: 2 · 2014: 5 · 2013: 2 · 2012: 5
- FWCI
- 26.59
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Health Sciences › Medicine › Hematology › Blood groups and transfusion
- Health Sciences › Medicine › Genetics › Hemoglobinopathies and Related Disorders
- Keywords
- Plasmodium falciparum, chondroitin sulfate A, chondroitin sulfate C, erythrocyte membrane protein 1, var genes, Duffy binding-like domain, placental malaria, placental infection, parasite adhesion, malaria in pregnancy, protective antibodies, immunological cross-reactivity
- MeSH terms
- Animals, Chondroitin Sulfates, Cloning, Molecular, Erythrocyte Membrane, Female, Cricetinae, Humans, Membrane Proteins, Molecular Sequence Data, Phenotype, Placenta, Plasmodium falciparum, Pregnancy, Trypsin, CHO Cells
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