The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
In vivo transposition of mariner -based elements in enteric bacteria and mycobacteria
Open accessAbstract
mariner family transposons are widespread among eukaryotic organisms. These transposons are apparently horizontally transmitted among diverse eukaryotes and can also transpose in vitro in the absence of added cofactors. Here we show that transposons derived from the mariner element Himar1 can efficiently transpose in bacteria in vivo . We have developed simple transposition systems by using minitransposons, made up of short inverted repeats flanking antibiotic resistance markers. These elements can efficiently transpose after expression of transposase from an appropriate bacterial promoter. We found that transposition of mariner -based elements in Escherichia coli produces diverse insertion mutations in either a targeted plasmid or a chromosomal gene. With Himar1 -derived transposons we were able to isolate phage-resistant mutants of both E. coli and Mycobacterium smegmatis . mariner- based transposons will provide valuable tools for mutagenesis and genetic manipulation of bacteria that currently lack well developed genetic systems.
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Genes required for mycobacterial growth defined by high density mutagenesis
Sassetti, C. M., Boyd, D. H., & Rubin, E. J. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03425.x · Cited by 2,544
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Self-citationComprehensive identification of conditionally essential genes in mycobacteria
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Metadata
- Authors
- Rubin, E. J., Akerley, B. J., Novik, V. N., Lampe, D. J., Husson, R. N., & Mekalanos, J. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-02-16
- Volume
- 96
- Issue
- 4
- Pages
- 1645-1650
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1996001643
- Cited by
- 358
- References
- 29
- FWCI
- 6.42
- Type
- journal article
- Language
- en
- Open access
- green
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- Rubin, E. J., Akerley, B. J., Novik, V. N., Lampe, D. J., Husson, R. N., & Mekalanos, J. J. (1999). In vivo transposition of mariner -based elements in enteric bacteria and mycobacteria. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.4.1645
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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