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Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery
Open accessAbstract
Poly(ethylenimine) (PEI) is one of a number of polycations that has been used successfully to transfer genes into living cells. Although PEI shows promise in the field of gene therapy, to date no rigorous proof of mechanism has been published regarding the fate of PEI/DNA administered for transfection. Here we show, by using fluorescent labeling and confocal microscopy, the paths of PEI/DNA complexes from endocytosis to gene expression. We found that complexes attach to cell surfaces and migrate into clumps that are endocytosed. The endocytotic vesicles grow in number and size and are occasionally seen to lyse. Most interesting is the fact that endocytosed PEI, whether administered with or without DNA, undergoes nuclear localization in the form of ordered structures.
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Cited by
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Yin, H., Kanasty, R. L., Eltoukhy, A. A., Vegas, A. J., Dorkin, J. R., & Anderson, D. G. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3763 · Cited by 3,123
Nonviral vectors for gene delivery
Mintzer, M. A., & Simanek, E. E. (2009) · Chemical Reviews · doi:10.1021/cr800409e · Cited by 2,366
Synthetic DNA delivery systems
Luo, D., & Saltzman, W. M. (2000) · Nature Biotechnology · doi:10.1038/71889 · Cited by 1,626
Xia, T., Kovochich, M., Liong, M., Meng, H., Kabehie, S., George, S., Zink, J. I., & Nel, A. E. (2009) · ACS Nano · doi:10.1021/nn900918w · Cited by 933
Nanotechnology with Soft Materials
Hamley, I. W. (2003) · Angewandte Chemie International Edition · doi:10.1002/anie.200200546 · Cited by 914
Metadata
- Authors
- Godbey, W. T., Wu, K. K., & Mikos, A. G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-04-27
- Volume
- 96
- Issue
- 9
- Pages
- 5177-5181
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2092447252
- Cited by
- 822
- References
- 0
- FWCI
- 8.92
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. https://app.cochat.ai/papers/10.1073/pnas.96.9.5177
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- Godbey, W. T., Wu, K. K., & Mikos, A. G. (1999). Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.9.5177
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